Hibiscus green spot virus (isolate Citrus volkameriana /USA/WAI 1-1/2009)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Kitaviridae; Higrevirus; unclassified Higrevirus; Hibiscus green spot virus

Average proteome isoelectric point is 7.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8DPK0|G8DPK0_HGSV2 p9 OS=Hibiscus green spot virus (isolate Citrus volkameriana /USA/WAI 1-1/2009) OX=1554497 PE=4 SV=1
MM1 pKa = 7.72EE2 pKa = 4.75SFNYY6 pKa = 7.68VTSADD11 pKa = 4.16LVCSLSRR18 pKa = 11.84FRR20 pKa = 11.84DD21 pKa = 4.52FYY23 pKa = 11.2KK24 pKa = 10.59EE25 pKa = 3.83RR26 pKa = 11.84SASLSDD32 pKa = 3.55DD33 pKa = 3.88DD34 pKa = 5.82VYY36 pKa = 11.95ADD38 pKa = 3.51TTRR41 pKa = 11.84EE42 pKa = 3.56YY43 pKa = 11.2SAALDD48 pKa = 3.38STEE51 pKa = 3.72FVDD54 pKa = 4.67YY55 pKa = 11.04NFQIFLLNGVPGGGKK70 pKa = 8.17TKK72 pKa = 10.26FVYY75 pKa = 10.63EE76 pKa = 4.03NVEE79 pKa = 3.88VRR81 pKa = 11.84NSCVVVPFKK90 pKa = 10.85ALKK93 pKa = 10.3DD94 pKa = 3.66EE95 pKa = 4.04YY96 pKa = 10.49RR97 pKa = 11.84KK98 pKa = 10.11RR99 pKa = 11.84GYY101 pKa = 10.61RR102 pKa = 11.84SFTQFRR108 pKa = 11.84ALPSVRR114 pKa = 11.84SADD117 pKa = 3.0ILVIDD122 pKa = 5.54EE123 pKa = 4.48YY124 pKa = 11.44TCVCYY129 pKa = 10.43SVLVSLVYY137 pKa = 10.45KK138 pKa = 10.52LRR140 pKa = 11.84PATVALIGDD149 pKa = 5.15FNQCWIRR156 pKa = 11.84DD157 pKa = 3.79GEE159 pKa = 4.47GFSMEE164 pKa = 4.5PFVSTLVVNKK174 pKa = 9.77YY175 pKa = 10.67LSVCYY180 pKa = 9.82RR181 pKa = 11.84CPCPDD186 pKa = 3.01VEE188 pKa = 4.94FVRR191 pKa = 11.84NYY193 pKa = 10.8LDD195 pKa = 5.44LDD197 pKa = 3.57ITPGKK202 pKa = 10.18CGSTCSCTGFNVEE215 pKa = 4.41TLVEE219 pKa = 4.77GMTFDD224 pKa = 3.7YY225 pKa = 10.42RR226 pKa = 11.84GEE228 pKa = 4.07VALVFSGASKK238 pKa = 10.8SYY240 pKa = 10.55LEE242 pKa = 4.67SIGVSAVTVRR252 pKa = 11.84SFMGKK257 pKa = 9.23QADD260 pKa = 4.01TVALFILKK268 pKa = 10.28DD269 pKa = 3.24HH270 pKa = 7.77DD271 pKa = 3.67IRR273 pKa = 11.84LLGVSSLKK281 pKa = 10.5LVAFTRR287 pKa = 11.84HH288 pKa = 3.91VSKK291 pKa = 10.96LVVYY295 pKa = 11.01SDD297 pKa = 3.82MSHH300 pKa = 7.29DD301 pKa = 4.1DD302 pKa = 3.53VVALIEE308 pKa = 4.38GATEE312 pKa = 3.78SGEE315 pKa = 4.62VVDD318 pKa = 5.58HH319 pKa = 7.98DD320 pKa = 4.27NFQPAYY326 pKa = 9.29EE327 pKa = 5.14DD328 pKa = 3.58SLEE331 pKa = 4.11QYY333 pKa = 10.78FDD335 pKa = 3.36RR336 pKa = 11.84LLRR339 pKa = 11.84GDD341 pKa = 4.45FYY343 pKa = 11.95VLL345 pKa = 3.41

Molecular weight: 38.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8DPK4|G8DPK4_HGSV2 p23 OS=Hibiscus green spot virus (isolate Citrus volkameriana /USA/WAI 1-1/2009) OX=1554497 PE=4 SV=1
MM1 pKa = 7.59LGLPEE6 pKa = 4.04LRR8 pKa = 11.84LRR10 pKa = 11.84LRR12 pKa = 11.84ARR14 pKa = 11.84GKK16 pKa = 10.89GLDD19 pKa = 3.44ALIYY23 pKa = 10.56DD24 pKa = 4.52VFNICLKK31 pKa = 10.88FIMKK35 pKa = 8.68PHH37 pKa = 6.52ILIMYY42 pKa = 8.08ACLLVVVYY50 pKa = 10.65NKK52 pKa = 10.92GEE54 pKa = 4.16AGDD57 pKa = 4.18VIEE60 pKa = 5.21KK61 pKa = 10.4ALKK64 pKa = 9.9AYY66 pKa = 8.41PRR68 pKa = 11.84NPILKK73 pKa = 8.56WAKK76 pKa = 7.64TDD78 pKa = 3.36YY79 pKa = 11.03VRR81 pKa = 11.84FIGILVGLPVIIDD94 pKa = 3.74APRR97 pKa = 11.84SFQLLITGAVFVSTYY112 pKa = 10.31ILPPQTLSTYY122 pKa = 10.92LLGFVSLHH130 pKa = 6.17VYY132 pKa = 10.5SKK134 pKa = 11.12AKK136 pKa = 10.07SVQLRR141 pKa = 11.84IILLASFATYY151 pKa = 10.49LVLSGIVDD159 pKa = 3.71TEE161 pKa = 4.13KK162 pKa = 10.23FTGSGTVLGGAHH174 pKa = 7.3GAPKK178 pKa = 10.21LSEE181 pKa = 4.15VPIPTVLNDD190 pKa = 3.84PGPRR194 pKa = 11.84IRR196 pKa = 11.84SVEE199 pKa = 3.64EE200 pKa = 3.41RR201 pKa = 11.84LPVIKK206 pKa = 10.32RR207 pKa = 11.84GGSGGVV213 pKa = 3.02

Molecular weight: 23.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

4327

47

2645

540.9

60.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.078 ± 0.571

2.843 ± 0.148

6.61 ± 0.989

4.923 ± 0.47

5.778 ± 0.907

6.517 ± 0.426

2.242 ± 0.47

4.761 ± 0.467

5.177 ± 0.945

9.591 ± 0.955

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.75 ± 0.248

3.028 ± 0.302

3.975 ± 0.369

1.849 ± 0.267

6.24 ± 0.592

7.788 ± 0.441

4.368 ± 0.222

10.192 ± 0.447

0.878 ± 0.295

4.414 ± 0.357
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski