Massilia sp. Leaf139

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Massilia; unclassified Massilia

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4544 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5HGW7|A0A0Q5HGW7_9BURK Diacylglycerol kinase OS=Massilia sp. Leaf139 OX=1736272 GN=ASF77_14020 PE=4 SV=1
MM1 pKa = 6.83TAVAEE6 pKa = 4.27AVDD9 pKa = 4.86FDD11 pKa = 5.17TIPSPIVFTDD21 pKa = 3.66SAAQKK26 pKa = 9.24VAQLIEE32 pKa = 4.37EE33 pKa = 4.51EE34 pKa = 4.63GNPDD38 pKa = 2.85LKK40 pKa = 11.13LRR42 pKa = 11.84VFVQGGGCSGFQYY55 pKa = 10.94GFTFDD60 pKa = 4.69EE61 pKa = 4.93IVNEE65 pKa = 4.96DD66 pKa = 3.85DD67 pKa = 3.19TTMEE71 pKa = 4.19KK72 pKa = 10.82NGVQLLIDD80 pKa = 3.77SMSYY84 pKa = 10.23QYY86 pKa = 11.55LVGAEE91 pKa = 4.01IDD93 pKa = 3.89YY94 pKa = 11.28KK95 pKa = 11.39DD96 pKa = 4.47DD97 pKa = 4.08LEE99 pKa = 4.56GAQFVIKK106 pKa = 10.53NPNATSTCGCGSSFSAA122 pKa = 4.79

Molecular weight: 13.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5HH95|A0A0Q5HH95_9BURK CoA-binding protein OS=Massilia sp. Leaf139 OX=1736272 GN=ASF77_11995 PE=4 SV=1
MM1 pKa = 7.22FFSGPPAVEE10 pKa = 4.16VEE12 pKa = 4.37DD13 pKa = 4.07GRR15 pKa = 11.84RR16 pKa = 11.84WYY18 pKa = 10.8QKK20 pKa = 6.54TCRR23 pKa = 11.84VRR25 pKa = 11.84VQPAAIKK32 pKa = 10.04RR33 pKa = 11.84VQLAQDD39 pKa = 3.44CQRR42 pKa = 11.84RR43 pKa = 11.84QTQASRR49 pKa = 11.84APVWLTVCFLVKK61 pKa = 10.51NMYY64 pKa = 9.92RR65 pKa = 3.42

Molecular weight: 7.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4544

0

4544

1539745

37

11403

338.9

36.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.121 ± 0.054

0.836 ± 0.012

5.338 ± 0.025

5.479 ± 0.033

3.565 ± 0.023

8.404 ± 0.039

2.117 ± 0.018

4.522 ± 0.026

3.459 ± 0.033

10.483 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.49 ± 0.02

2.945 ± 0.02

5.084 ± 0.028

3.688 ± 0.021

6.83 ± 0.035

5.287 ± 0.028

5.218 ± 0.033

7.446 ± 0.031

1.286 ± 0.015

2.404 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski