Amino acid dipepetide frequency for Cuculus canorus (common cuckoo)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.845AlaAla: 5.845 ± 0.052	1.383AlaCys: 1.383 ± 0.021	3.013AlaAsp: 3.013 ± 0.026	4.618AlaGlu: 4.618 ± 0.03	2.647AlaPhe: 2.647 ± 0.026	4.003AlaGly: 4.003 ± 0.031	1.415AlaHis: 1.415 ± 0.017	3.088AlaIle: 3.088 ± 0.026	3.676AlaLys: 3.676 ± 0.029	6.509AlaLeu: 6.509 ± 0.045	1.523AlaMet: 1.523 ± 0.016	2.252AlaAsn: 2.252 ± 0.022	2.969AlaPro: 2.969 ± 0.026	2.772AlaGln: 2.772 ± 0.028	3.043AlaArg: 3.043 ± 0.029	5.189AlaSer: 5.189 ± 0.036	3.382AlaThr: 3.382 ± 0.026	4.985AlaVal: 4.985 ± 0.035	0.725AlaTrp: 0.725 ± 0.012	1.645AlaTyr: 1.645 ± 0.018	0.0AlaXaa: 0.0 ± 0.0
Cys	1.22CysAla: 1.22 ± 0.02	0.658CysCys: 0.658 ± 0.013	1.097CysAsp: 1.097 ± 0.023	1.341CysGlu: 1.341 ± 0.022	0.939CysPhe: 0.939 ± 0.013	1.516CysGly: 1.516 ± 0.025	0.645CysHis: 0.645 ± 0.011	1.163CysIle: 1.163 ± 0.021	1.34CysLys: 1.34 ± 0.017	2.255CysLeu: 2.255 ± 0.027	0.477CysMet: 0.477 ± 0.01	0.928CysAsn: 0.928 ± 0.016	1.325CysPro: 1.325 ± 0.026	1.084CysGln: 1.084 ± 0.018	1.259CysArg: 1.259 ± 0.018	2.054CysSer: 2.054 ± 0.025	1.253CysThr: 1.253 ± 0.021	1.458CysVal: 1.458 ± 0.027	0.299CysTrp: 0.299 ± 0.008	0.679CysTyr: 0.679 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	2.945AspAla: 2.945 ± 0.024	1.138AspCys: 1.138 ± 0.018	2.861AspAsp: 2.861 ± 0.031	3.683AspGlu: 3.683 ± 0.031	2.303AspPhe: 2.303 ± 0.022	3.36AspGly: 3.36 ± 0.034	1.171AspHis: 1.171 ± 0.014	2.973AspIle: 2.973 ± 0.027	2.761AspLys: 2.761 ± 0.024	5.006AspLeu: 5.006 ± 0.029	1.164AspMet: 1.164 ± 0.012	1.931AspAsn: 1.931 ± 0.022	2.684AspPro: 2.684 ± 0.021	1.823AspGln: 1.823 ± 0.02	2.393AspArg: 2.393 ± 0.026	4.072AspSer: 4.072 ± 0.03	2.511AspThr: 2.511 ± 0.023	3.259AspVal: 3.259 ± 0.026	0.659AspTrp: 0.659 ± 0.012	1.674AspTyr: 1.674 ± 0.017	0.0AspXaa: 0.0 ± 0.0
Glu	4.694GluAla: 4.694 ± 0.035	1.355GluCys: 1.355 ± 0.026	4.506GluAsp: 4.506 ± 0.037	7.858GluGlu: 7.858 ± 0.077	2.216GluPhe: 2.216 ± 0.024	3.931GluGly: 3.931 ± 0.032	1.548GluHis: 1.548 ± 0.018	3.511GluIle: 3.511 ± 0.025	5.654GluLys: 5.654 ± 0.05	6.386GluLeu: 6.386 ± 0.044	1.79GluMet: 1.79 ± 0.017	3.421GluAsn: 3.421 ± 0.027	2.577GluPro: 2.577 ± 0.024	3.204GluGln: 3.204 ± 0.032	3.946GluArg: 3.946 ± 0.045	4.452GluSer: 4.452 ± 0.029	3.514GluThr: 3.514 ± 0.024	4.342GluVal: 4.342 ± 0.03	0.745GluTrp: 0.745 ± 0.012	1.831GluTyr: 1.831 ± 0.017	0.0GluXaa: 0.0 ± 0.0
Phe	2.21PheAla: 2.21 ± 0.022	1.007PheCys: 1.007 ± 0.015	1.84PheAsp: 1.84 ± 0.021	2.156PheGlu: 2.156 ± 0.024	1.838PhePhe: 1.838 ± 0.022	2.339PheGly: 2.339 ± 0.024	1.114PheHis: 1.114 ± 0.013	2.123PheIle: 2.123 ± 0.022	2.035PheLys: 2.035 ± 0.021	4.208PheLeu: 4.208 ± 0.034	0.801PheMet: 0.801 ± 0.013	1.535PheAsn: 1.535 ± 0.018	1.957PhePro: 1.957 ± 0.021	1.841PheGln: 1.841 ± 0.016	1.954PheArg: 1.954 ± 0.022	3.453PheSer: 3.453 ± 0.024	2.253PheThr: 2.253 ± 0.02	2.424PheVal: 2.424 ± 0.026	0.524PheTrp: 0.524 ± 0.011	1.347PheTyr: 1.347 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	3.621GlyAla: 3.621 ± 0.033	1.281GlyCys: 1.281 ± 0.017	2.996GlyAsp: 2.996 ± 0.026	3.791GlyGlu: 3.791 ± 0.038	2.506GlyPhe: 2.506 ± 0.025	4.01GlyGly: 4.01 ± 0.041	1.541GlyHis: 1.541 ± 0.02	3.106GlyIle: 3.106 ± 0.027	4.022GlyLys: 4.022 ± 0.028	5.233GlyLeu: 5.233 ± 0.034	1.402GlyMet: 1.402 ± 0.019	2.607GlyAsn: 2.607 ± 0.024	2.743GlyPro: 2.743 ± 0.05	2.529GlyGln: 2.529 ± 0.026	3.232GlyArg: 3.232 ± 0.033	5.135GlySer: 5.135 ± 0.039	3.491GlyThr: 3.491 ± 0.031	3.527GlyVal: 3.527 ± 0.029	0.774GlyTrp: 0.774 ± 0.013	1.939GlyTyr: 1.939 ± 0.025	0.0GlyXaa: 0.0 ± 0.0
His	1.362HisAla: 1.362 ± 0.018	0.725HisCys: 0.725 ± 0.013	0.942HisAsp: 0.942 ± 0.013	1.356HisGlu: 1.356 ± 0.016	1.084HisPhe: 1.084 ± 0.013	1.532HisGly: 1.532 ± 0.02	0.901HisHis: 0.901 ± 0.016	1.367HisIle: 1.367 ± 0.016	1.378HisLys: 1.378 ± 0.016	2.789HisLeu: 2.789 ± 0.024	0.603HisMet: 0.603 ± 0.011	0.98HisAsn: 0.98 ± 0.014	1.529HisPro: 1.529 ± 0.018	1.199HisGln: 1.199 ± 0.015	1.502HisArg: 1.502 ± 0.015	2.193HisSer: 2.193 ± 0.022	1.36HisThr: 1.36 ± 0.021	1.548HisVal: 1.548 ± 0.019	0.372HisTrp: 0.372 ± 0.008	0.887HisTyr: 0.887 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	3.039IleAla: 3.039 ± 0.023	1.243IleCys: 1.243 ± 0.017	2.36IleAsp: 2.36 ± 0.024	2.914IleGlu: 2.914 ± 0.025	2.177IlePhe: 2.177 ± 0.027	2.505IleGly: 2.505 ± 0.024	1.405IleHis: 1.405 ± 0.015	2.753IleIle: 2.753 ± 0.027	2.997IleLys: 2.997 ± 0.025	5.019IleLeu: 5.019 ± 0.036	1.065IleMet: 1.065 ± 0.015	2.166IleAsn: 2.166 ± 0.02	2.853IlePro: 2.853 ± 0.023	2.473IleGln: 2.473 ± 0.023	2.593IleArg: 2.593 ± 0.022	4.089IleSer: 4.089 ± 0.025	2.889IleThr: 2.889 ± 0.025	2.949IleVal: 2.949 ± 0.023	0.586IleTrp: 0.586 ± 0.011	1.64IleTyr: 1.64 ± 0.021	0.0IleXaa: 0.0 ± 0.0
Lys	4.134LysAla: 4.134 ± 0.031	1.246LysCys: 1.246 ± 0.018	3.44LysAsp: 3.44 ± 0.03	5.601LysGlu: 5.601 ± 0.043	1.893LysPhe: 1.893 ± 0.02	3.463LysGly: 3.463 ± 0.031	1.561LysHis: 1.561 ± 0.02	3.16LysIle: 3.16 ± 0.027	5.294LysLys: 5.294 ± 0.051	5.642LysLeu: 5.642 ± 0.033	1.546LysMet: 1.546 ± 0.015	2.735LysAsn: 2.735 ± 0.022	3.062LysPro: 3.062 ± 0.028	2.924LysGln: 2.924 ± 0.027	3.557LysArg: 3.557 ± 0.029	4.216LysSer: 4.216 ± 0.041	3.35LysThr: 3.35 ± 0.025	3.722LysVal: 3.722 ± 0.028	0.694LysTrp: 0.694 ± 0.013	1.873LysTyr: 1.873 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	6.312LeuAla: 6.312 ± 0.039	2.261LeuCys: 2.261 ± 0.025	4.856LeuAsp: 4.856 ± 0.035	7.06LeuGlu: 7.06 ± 0.056	3.602LeuPhe: 3.602 ± 0.035	5.27LeuGly: 5.27 ± 0.035	2.692LeuHis: 2.692 ± 0.028	4.274LeuIle: 4.274 ± 0.034	6.338LeuLys: 6.338 ± 0.041	10.115LeuLeu: 10.115 ± 0.071	2.044LeuMet: 2.044 ± 0.018	3.872LeuAsn: 3.872 ± 0.025	5.428LeuPro: 5.428 ± 0.036	5.6LeuGln: 5.6 ± 0.037	5.232LeuArg: 5.232 ± 0.034	7.843LeuSer: 7.843 ± 0.037	4.984LeuThr: 4.984 ± 0.031	5.529LeuVal: 5.529 ± 0.04	1.101LeuTrp: 1.101 ± 0.015	2.824LeuTyr: 2.824 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	1.663MetAla: 1.663 ± 0.02	0.454MetCys: 0.454 ± 0.01	1.304MetAsp: 1.304 ± 0.016	1.896MetGlu: 1.896 ± 0.021	0.83MetPhe: 0.83 ± 0.012	1.267MetGly: 1.267 ± 0.017	0.526MetHis: 0.526 ± 0.009	0.972MetIle: 0.972 ± 0.014	1.596MetLys: 1.596 ± 0.017	2.076MetLeu: 2.076 ± 0.019	0.598MetMet: 0.598 ± 0.011	1.0MetAsn: 1.0 ± 0.013	1.051MetPro: 1.051 ± 0.013	1.027MetGln: 1.027 ± 0.017	1.081MetArg: 1.081 ± 0.013	1.572MetSer: 1.572 ± 0.019	1.146MetThr: 1.146 ± 0.014	1.457MetVal: 1.457 ± 0.015	0.251MetTrp: 0.251 ± 0.006	0.667MetTyr: 0.667 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.351AsnAla: 2.351 ± 0.018	1.002AsnCys: 1.002 ± 0.017	1.736AsnAsp: 1.736 ± 0.019	2.567AsnGlu: 2.567 ± 0.025	1.629AsnPhe: 1.629 ± 0.017	2.778AsnGly: 2.778 ± 0.029	0.998AsnHis: 0.998 ± 0.014	2.493AsnIle: 2.493 ± 0.022	2.554AsnLys: 2.554 ± 0.021	4.026AsnLeu: 4.026 ± 0.031	1.001AsnMet: 1.001 ± 0.013	1.831AsnAsn: 1.831 ± 0.019	2.273AsnPro: 2.273 ± 0.022	1.708AsnGln: 1.708 ± 0.02	2.01AsnArg: 2.01 ± 0.016	3.414AsnSer: 3.414 ± 0.032	2.238AsnThr: 2.238 ± 0.021	2.501AsnVal: 2.501 ± 0.021	0.501AsnTrp: 0.501 ± 0.009	1.316AsnTyr: 1.316 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	3.747ProAla: 3.747 ± 0.034	1.124ProCys: 1.124 ± 0.02	2.636ProAsp: 2.636 ± 0.02	3.866ProGlu: 3.866 ± 0.029	1.937ProPhe: 1.937 ± 0.02	3.673ProGly: 3.673 ± 0.068	1.269ProHis: 1.269 ± 0.017	1.998ProIle: 1.998 ± 0.019	2.734ProLys: 2.734 ± 0.025	4.683ProLeu: 4.683 ± 0.037	0.944ProMet: 0.944 ± 0.013	1.898ProAsn: 1.898 ± 0.021	4.301ProPro: 4.301 ± 0.061	2.335ProGln: 2.335 ± 0.028	2.537ProArg: 2.537 ± 0.024	4.881ProSer: 4.881 ± 0.047	2.692ProThr: 2.692 ± 0.027	3.736ProVal: 3.736 ± 0.03	0.59ProTrp: 0.59 ± 0.012	1.487ProTyr: 1.487 ± 0.017	0.001ProXaa: 0.001 ± 0.0
Gln	3.037GlnAla: 3.037 ± 0.031	0.996GlnCys: 0.996 ± 0.018	2.233GlnAsp: 2.233 ± 0.018	3.652GlnGlu: 3.652 ± 0.032	1.49GlnPhe: 1.49 ± 0.016	2.496GlnGly: 2.496 ± 0.025	1.3GlnHis: 1.3 ± 0.016	2.218GlnIle: 2.218 ± 0.02	3.105GlnLys: 3.105 ± 0.028	4.617GlnLeu: 4.617 ± 0.041	1.118GlnMet: 1.118 ± 0.015	2.025GlnAsn: 2.025 ± 0.02	2.379GlnPro: 2.379 ± 0.033	3.009GlnGln: 3.009 ± 0.05	2.702GlnArg: 2.702 ± 0.027	3.168GlnSer: 3.168 ± 0.028	2.352GlnThr: 2.352 ± 0.024	2.767GlnVal: 2.767 ± 0.026	0.539GlnTrp: 0.539 ± 0.01	1.289GlnTyr: 1.289 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	3.134ArgAla: 3.134 ± 0.025	1.181ArgCys: 1.181 ± 0.021	2.627ArgAsp: 2.627 ± 0.027	3.744ArgGlu: 3.744 ± 0.038	1.934ArgPhe: 1.934 ± 0.018	2.944ArgGly: 2.944 ± 0.032	1.479ArgHis: 1.479 ± 0.016	2.646ArgIle: 2.646 ± 0.025	3.91ArgLys: 3.91 ± 0.035	4.942ArgLeu: 4.942 ± 0.034	1.191ArgMet: 1.191 ± 0.017	2.266ArgAsn: 2.266 ± 0.021	2.426ArgPro: 2.426 ± 0.023	2.508ArgGln: 2.508 ± 0.027	3.793ArgArg: 3.793 ± 0.039	3.978ArgSer: 3.978 ± 0.037	2.713ArgThr: 2.713 ± 0.023	2.988ArgVal: 2.988 ± 0.026	0.637ArgTrp: 0.637 ± 0.011	1.645ArgTyr: 1.645 ± 0.018	0.0ArgXaa: 0.0 ± 0.0
Ser	5.099SerAla: 5.099 ± 0.035	1.891SerCys: 1.891 ± 0.023	3.942SerAsp: 3.942 ± 0.031	5.065SerGlu: 5.065 ± 0.036	3.155SerPhe: 3.155 ± 0.026	4.994SerGly: 4.994 ± 0.038	2.031SerHis: 2.031 ± 0.022	3.62SerIle: 3.62 ± 0.028	4.412SerLys: 4.412 ± 0.033	7.997SerLeu: 7.997 ± 0.047	1.625SerMet: 1.625 ± 0.017	3.012SerAsn: 3.012 ± 0.026	5.107SerPro: 5.107 ± 0.049	3.606SerGln: 3.606 ± 0.032	4.108SerArg: 4.108 ± 0.039	9.038SerSer: 9.038 ± 0.074	4.514SerThr: 4.514 ± 0.035	5.14SerVal: 5.14 ± 0.038	0.99SerTrp: 0.99 ± 0.014	2.264SerTyr: 2.264 ± 0.024	0.001SerXaa: 0.001 ± 0.0
Thr	3.832ThrAla: 3.832 ± 0.026	1.357ThrCys: 1.357 ± 0.025	2.724ThrAsp: 2.724 ± 0.023	3.675ThrGlu: 3.675 ± 0.028	2.196ThrPhe: 2.196 ± 0.022	3.492ThrGly: 3.492 ± 0.026	1.235ThrHis: 1.235 ± 0.02	2.637ThrIle: 2.637 ± 0.022	2.858ThrLys: 2.858 ± 0.021	5.157ThrLeu: 5.157 ± 0.023	1.142ThrMet: 1.142 ± 0.014	1.963ThrAsn: 1.963 ± 0.019	3.077ThrPro: 3.077 ± 0.031	2.143ThrGln: 2.143 ± 0.022	2.355ThrArg: 2.355 ± 0.02	4.579ThrSer: 4.579 ± 0.033	3.015ThrThr: 3.015 ± 0.029	4.133ThrVal: 4.133 ± 0.029	0.685ThrTrp: 0.685 ± 0.011	1.584ThrTyr: 1.584 ± 0.017	0.0ThrXaa: 0.0 ± 0.0
Val	4.162ValAla: 4.162 ± 0.032	1.654ValCys: 1.654 ± 0.026	3.084ValAsp: 3.084 ± 0.026	3.998ValGlu: 3.998 ± 0.028	2.702ValPhe: 2.702 ± 0.025	3.406ValGly: 3.406 ± 0.031	1.626ValHis: 1.626 ± 0.018	3.317ValIle: 3.317 ± 0.025	3.82ValLys: 3.82 ± 0.029	6.421ValLeu: 6.421 ± 0.036	1.393ValMet: 1.393 ± 0.015	2.57ValAsn: 2.57 ± 0.026	3.515ValPro: 3.515 ± 0.03	2.82ValGln: 2.82 ± 0.022	3.001ValArg: 3.001 ± 0.025	4.956ValSer: 4.956 ± 0.036	3.857ValThr: 3.857 ± 0.03	4.314ValVal: 4.314 ± 0.031	0.745ValTrp: 0.745 ± 0.013	1.894ValTyr: 1.894 ± 0.022	0.0ValXaa: 0.0 ± 0.0
Trp	0.686TrpAla: 0.686 ± 0.012	0.258TrpCys: 0.258 ± 0.007	0.684TrpAsp: 0.684 ± 0.014	0.798TrpGlu: 0.798 ± 0.014	0.476TrpPhe: 0.476 ± 0.01	0.652TrpGly: 0.652 ± 0.014	0.34TrpHis: 0.34 ± 0.008	0.636TrpIle: 0.636 ± 0.011	0.9TrpLys: 0.9 ± 0.01	1.19TrpLeu: 1.19 ± 0.016	0.32TrpMet: 0.32 ± 0.008	0.623TrpAsn: 0.623 ± 0.012	0.435TrpPro: 0.435 ± 0.008	0.559TrpGln: 0.559 ± 0.009	0.661TrpArg: 0.661 ± 0.011	0.898TrpSer: 0.898 ± 0.015	0.66TrpThr: 0.66 ± 0.014	0.682TrpVal: 0.682 ± 0.012	0.2TrpTrp: 0.2 ± 0.006	0.379TrpTyr: 0.379 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.588TyrAla: 1.588 ± 0.02	0.775TyrCys: 0.775 ± 0.012	1.493TyrAsp: 1.493 ± 0.018	1.842TyrGlu: 1.842 ± 0.019	1.407TyrPhe: 1.407 ± 0.016	1.854TyrGly: 1.854 ± 0.021	0.811TyrHis: 0.811 ± 0.012	1.659TyrIle: 1.659 ± 0.019	1.727TyrLys: 1.727 ± 0.018	2.967TyrLeu: 2.967 ± 0.029	0.684TyrMet: 0.684 ± 0.011	1.31TyrAsn: 1.31 ± 0.017	1.377TyrPro: 1.377 ± 0.017	1.327TyrGln: 1.327 ± 0.016	1.73TyrArg: 1.73 ± 0.018	2.427TyrSer: 2.427 ± 0.024	1.668TyrThr: 1.668 ± 0.019	1.796TyrVal: 1.796 ± 0.017	0.42TyrTrp: 0.42 ± 0.011	1.124TyrTyr: 1.124 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.02XaaXaa: 0.02 ± 0.004

Statistics based on 13582 proteins (6119957 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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