Black robin associated gemykibivirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus blaro1

Average proteome isoelectric point is 7.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T1YTB1|T1YTB1_9VIRU Capsid protein OS=Black robin associated gemykibivirus 1 OX=1391037 PE=4 SV=1
MM1 pKa = 7.58SFRR4 pKa = 11.84FAAKK8 pKa = 9.17WGLLTFPQSGDD19 pKa = 3.37LDD21 pKa = 3.46PWEE24 pKa = 4.67VNDD27 pKa = 3.89HH28 pKa = 6.64LGRR31 pKa = 11.84LGAEE35 pKa = 4.19CIIGRR40 pKa = 11.84EE41 pKa = 3.94EE42 pKa = 3.89HH43 pKa = 7.07ADD45 pKa = 3.59GGLHH49 pKa = 5.95LHH51 pKa = 6.48AFFMFEE57 pKa = 4.26RR58 pKa = 11.84KK59 pKa = 9.22FEE61 pKa = 4.24SRR63 pKa = 11.84NVRR66 pKa = 11.84VFDD69 pKa = 3.45VDD71 pKa = 3.28GRR73 pKa = 11.84HH74 pKa = 5.89PNVVRR79 pKa = 11.84GYY81 pKa = 7.82STPEE85 pKa = 3.33KK86 pKa = 10.04GATYY90 pKa = 10.03AIKK93 pKa = 10.89DD94 pKa = 3.57GDD96 pKa = 4.14VVAGGLDD103 pKa = 3.73PASLGGDD110 pKa = 2.96RR111 pKa = 11.84VDD113 pKa = 4.75RR114 pKa = 11.84VADD117 pKa = 3.95PWPAIILSTTRR128 pKa = 11.84DD129 pKa = 3.6EE130 pKa = 4.86FFEE133 pKa = 4.4ACARR137 pKa = 11.84MAPKK141 pKa = 10.52SLVCSFNSLKK151 pKa = 10.72CYY153 pKa = 10.53ADD155 pKa = 2.72WRR157 pKa = 11.84YY158 pKa = 9.95RR159 pKa = 11.84PEE161 pKa = 4.12PVPYY165 pKa = 8.89EE166 pKa = 4.25HH167 pKa = 7.51PSGVQICTDD176 pKa = 4.15RR177 pKa = 11.84FPDD180 pKa = 3.66LSSWVLQNLEE190 pKa = 4.03RR191 pKa = 11.84RR192 pKa = 11.84TSGRR196 pKa = 11.84RR197 pKa = 11.84RR198 pKa = 11.84SLVLYY203 pKa = 10.58GPTRR207 pKa = 11.84LGKK210 pKa = 7.5TLWARR215 pKa = 11.84SLGNHH220 pKa = 7.12AYY222 pKa = 10.41FGGLFSLEE230 pKa = 4.18EE231 pKa = 3.98EE232 pKa = 4.7LEE234 pKa = 4.33GVDD237 pKa = 4.11YY238 pKa = 11.31AIFDD242 pKa = 4.11DD243 pKa = 4.11MQGGLKK249 pKa = 10.09FFHH252 pKa = 7.11SYY254 pKa = 10.7KK255 pKa = 10.25FWLGAQSQFWATDD268 pKa = 2.95KK269 pKa = 11.37YY270 pKa = 10.54RR271 pKa = 11.84GKK273 pKa = 10.83KK274 pKa = 8.9LIHH277 pKa = 6.62WGRR280 pKa = 11.84PSIYY284 pKa = 9.68ICNQNPLCDD293 pKa = 3.74EE294 pKa = 4.6GVDD297 pKa = 3.91HH298 pKa = 7.77DD299 pKa = 4.55WLIGNCDD306 pKa = 3.26FVEE309 pKa = 4.24ITEE312 pKa = 4.27SLLVPEE318 pKa = 5.05EE319 pKa = 4.32GG320 pKa = 3.94

Molecular weight: 36.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T1YTB1|T1YTB1_9VIRU Capsid protein OS=Black robin associated gemykibivirus 1 OX=1391037 PE=4 SV=1
MM1 pKa = 7.72AYY3 pKa = 10.17ARR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84FTSSRR12 pKa = 11.84ARR14 pKa = 11.84KK15 pKa = 8.46PIRR18 pKa = 11.84RR19 pKa = 11.84SRR21 pKa = 11.84RR22 pKa = 11.84PAGKK26 pKa = 8.67RR27 pKa = 11.84RR28 pKa = 11.84SYY30 pKa = 10.65RR31 pKa = 11.84RR32 pKa = 11.84STRR35 pKa = 11.84KK36 pKa = 9.65RR37 pKa = 11.84GMSTRR42 pKa = 11.84TLLNKK47 pKa = 9.52TSQKK51 pKa = 10.41KK52 pKa = 9.12RR53 pKa = 11.84DD54 pKa = 3.61NMLSFSNTTAEE65 pKa = 4.83DD66 pKa = 4.11PFSTDD71 pKa = 3.1YY72 pKa = 11.17TIGPAVMRR80 pKa = 11.84RR81 pKa = 11.84PVGADD86 pKa = 3.39LPAEE90 pKa = 4.97FIYY93 pKa = 10.52VWNATGRR100 pKa = 11.84PHH102 pKa = 6.78EE103 pKa = 4.7LSTGFRR109 pKa = 11.84GSKK112 pKa = 9.67IDD114 pKa = 3.28TSLRR118 pKa = 11.84TNTTIFAVGLKK129 pKa = 10.01EE130 pKa = 4.46RR131 pKa = 11.84IKK133 pKa = 11.3LEE135 pKa = 4.44TNNSAPWRR143 pKa = 11.84WRR145 pKa = 11.84RR146 pKa = 11.84ICFTSKK152 pKa = 10.59NDD154 pKa = 3.53YY155 pKa = 11.27GEE157 pKa = 4.97ADD159 pKa = 4.21PDD161 pKa = 3.33EE162 pKa = 5.37SDD164 pKa = 3.53YY165 pKa = 11.01FRR167 pKa = 11.84RR168 pKa = 11.84TSNGMVRR175 pKa = 11.84LLRR178 pKa = 11.84AQSQAEE184 pKa = 4.46EE185 pKa = 4.27INDD188 pKa = 3.85DD189 pKa = 3.76VFDD192 pKa = 4.11GQRR195 pKa = 11.84NVDD198 pKa = 3.41WLSAITAPINRR209 pKa = 11.84RR210 pKa = 11.84HH211 pKa = 5.62VSVHH215 pKa = 5.13YY216 pKa = 10.69DD217 pKa = 3.19RR218 pKa = 11.84TRR220 pKa = 11.84TIQSGNQSGVTRR232 pKa = 11.84TYY234 pKa = 10.51RR235 pKa = 11.84QWHH238 pKa = 5.74PMRR241 pKa = 11.84KK242 pKa = 8.28NVVYY246 pKa = 10.61ADD248 pKa = 3.85EE249 pKa = 4.16QAGEE253 pKa = 4.32GMVDD257 pKa = 3.08SGVSVTGKK265 pKa = 10.38VGMGNYY271 pKa = 10.03YY272 pKa = 11.15VMDD275 pKa = 4.08IFTKK279 pKa = 10.52HH280 pKa = 5.92GLNDD284 pKa = 3.79DD285 pKa = 3.77QSTLSYY291 pKa = 10.27EE292 pKa = 4.2PEE294 pKa = 4.09STFFWHH300 pKa = 6.76EE301 pKa = 3.87KK302 pKa = 9.8

Molecular weight: 34.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

622

302

320

311.0

35.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.109 ± 0.098

1.447 ± 0.734

6.913 ± 0.409

5.627 ± 0.652

5.145 ± 0.771

8.199 ± 1.038

2.572 ± 0.385

3.859 ± 0.076

4.18 ± 0.3

6.913 ± 1.717

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.09 ± 0.586

4.019 ± 0.841

4.662 ± 0.453

2.572 ± 0.268

9.968 ± 1.721

7.556 ± 0.911

6.109 ± 1.863

5.788 ± 0.104

2.572 ± 0.385

3.698 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski