Hubei virga-like virus 23

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJZ1|A0A1L3KJZ1_9VIRU Uncharacterized protein OS=Hubei virga-like virus 23 OX=1923338 PE=4 SV=1
MM1 pKa = 7.27QLILTLTFSLTLIAMTTAQLRR22 pKa = 11.84EE23 pKa = 3.85IFPRR27 pKa = 11.84YY28 pKa = 9.61DD29 pKa = 3.0VARR32 pKa = 11.84GNFEE36 pKa = 5.13DD37 pKa = 5.13DD38 pKa = 3.75ALANLYY44 pKa = 10.61DD45 pKa = 4.74RR46 pKa = 11.84GALTNSADD54 pKa = 3.66INSTCDD60 pKa = 2.87SYY62 pKa = 12.1GCSTEE67 pKa = 4.14FSHH70 pKa = 6.73TFNFNAQRR78 pKa = 11.84ASGTKK83 pKa = 8.86LTLTNNGLQNSTIVISVAEE102 pKa = 3.96ARR104 pKa = 11.84YY105 pKa = 9.57SVSLQYY111 pKa = 11.13LYY113 pKa = 10.42TIYY116 pKa = 9.85PAYY119 pKa = 9.91MFAGWASYY127 pKa = 10.78NYY129 pKa = 10.12EE130 pKa = 4.07EE131 pKa = 5.31NNYY134 pKa = 8.39NQCKK138 pKa = 9.8VKK140 pKa = 10.76YY141 pKa = 9.57DD142 pKa = 3.02WRR144 pKa = 11.84DD145 pKa = 3.15QVNARR150 pKa = 11.84FEE152 pKa = 4.12KK153 pKa = 11.12LEE155 pKa = 4.65DD156 pKa = 3.5YY157 pKa = 10.91VDD159 pKa = 3.1NYY161 pKa = 10.85RR162 pKa = 11.84RR163 pKa = 11.84YY164 pKa = 10.35QIVGSDD170 pKa = 3.5WLRR173 pKa = 11.84VDD175 pKa = 3.38YY176 pKa = 10.74AAWGSVATKK185 pKa = 10.44VCNHH189 pKa = 5.33YY190 pKa = 9.6WATTFTLCQRR200 pKa = 11.84KK201 pKa = 7.06TLVADD206 pKa = 4.69FGEE209 pKa = 5.29GIAVFKK215 pKa = 10.52IAPEE219 pKa = 4.15TTLTMILNIAINDD232 pKa = 3.67DD233 pKa = 3.64VKK235 pKa = 11.23NIEE238 pKa = 4.26VQPSIDD244 pKa = 3.72VPTEE248 pKa = 3.42ITLGDD253 pKa = 3.84LTLSISPAGTIISPLANNYY272 pKa = 9.44YY273 pKa = 10.18LAHH276 pKa = 6.36FQRR279 pKa = 11.84KK280 pKa = 7.39EE281 pKa = 3.8MFDD284 pKa = 3.89TISGAKK290 pKa = 8.19WYY292 pKa = 9.37KK293 pKa = 10.33ANSVPTISSISEE305 pKa = 4.39TITKK309 pKa = 9.23YY310 pKa = 10.41HH311 pKa = 6.42SPNLFPMNAYY321 pKa = 9.65KK322 pKa = 10.14PDD324 pKa = 3.91SEE326 pKa = 4.62TLQQFYY332 pKa = 10.12FDD334 pKa = 4.87KK335 pKa = 10.82MLLQGVNVVKK345 pKa = 10.57CGSSFEE351 pKa = 4.25QEE353 pKa = 3.97ANAKK357 pKa = 10.39NSVQRR362 pKa = 11.84RR363 pKa = 11.84LSKK366 pKa = 10.67LRR368 pKa = 11.84AEE370 pKa = 4.59AAAEE374 pKa = 3.94PSTTKK379 pKa = 9.44MDD381 pKa = 4.5SIISYY386 pKa = 10.61LPTLSNTNPPQTKK399 pKa = 9.2TYY401 pKa = 10.5FNDD404 pKa = 4.23PYY406 pKa = 11.29DD407 pKa = 4.44PYY409 pKa = 11.4DD410 pKa = 3.47NTSISTRR417 pKa = 11.84RR418 pKa = 11.84DD419 pKa = 3.02SDD421 pKa = 3.73CCIPITTEE429 pKa = 3.37SRR431 pKa = 11.84SYY433 pKa = 9.65ITTLLKK439 pKa = 11.03SFDD442 pKa = 4.25LVSDD446 pKa = 3.34IDD448 pKa = 3.74GQYY451 pKa = 11.0EE452 pKa = 3.99VDD454 pKa = 3.69TPSSITQFPGYY465 pKa = 9.99AYY467 pKa = 9.65PNAITMIANNLMKK480 pKa = 10.61VSLSAVLSSQSMRR493 pKa = 11.84IPYY496 pKa = 9.6SAADD500 pKa = 3.72GLVADD505 pKa = 4.11VSIPIMQITTSSAGSYY521 pKa = 10.45FNYY524 pKa = 9.62EE525 pKa = 3.39INYY528 pKa = 9.89VGTANLIPVSARR540 pKa = 11.84HH541 pKa = 5.64INLLKK546 pKa = 9.95RR547 pKa = 11.84TISVTSTGTYY557 pKa = 9.37SGTIAFVSPPDD568 pKa = 3.7PSVDD572 pKa = 3.36LTVCFGSNSVCIKK585 pKa = 10.45PKK587 pKa = 9.02VTNFLTPPQGAVQDD601 pKa = 4.63PDD603 pKa = 3.41TTTCSGVPPSFFSIIITNSGLLAISIIMIIVDD635 pKa = 4.57IILGIVILILLIRR648 pKa = 11.84IVPKK652 pKa = 10.37TFMLFLLLFSTKK664 pKa = 9.46TNALSYY670 pKa = 9.29TAAPISNLTLLDD682 pKa = 4.23NPALDD687 pKa = 3.97NARR690 pKa = 11.84LIYY693 pKa = 10.52SSGISYY699 pKa = 7.65STKK702 pKa = 10.61CEE704 pKa = 3.96TQSCSEE710 pKa = 4.06YY711 pKa = 10.26KK712 pKa = 10.39YY713 pKa = 10.51QSSSPKK719 pKa = 9.21PVEE722 pKa = 3.92YY723 pKa = 10.41TPDD726 pKa = 3.06AKK728 pKa = 10.83MNVYY732 pKa = 10.41LNNYY736 pKa = 8.5AKK738 pKa = 10.82LFAICHH744 pKa = 5.63NFAHH748 pKa = 6.84DD749 pKa = 3.23TGQRR753 pKa = 11.84LPYY756 pKa = 9.28NTVPTPNIDD765 pKa = 3.73LPKK768 pKa = 9.48SWFYY772 pKa = 10.86TIYY775 pKa = 9.84DD776 pKa = 4.0TVDD779 pKa = 3.03SKK781 pKa = 11.56PFVLSLPPYY790 pKa = 10.06LPSTRR795 pKa = 11.84GNYY798 pKa = 7.48VTDD801 pKa = 3.95RR802 pKa = 11.84GLFSPIVSGYY812 pKa = 7.76ATRR815 pKa = 11.84TEE817 pKa = 4.42DD818 pKa = 3.08NTYY821 pKa = 11.63ANITRR826 pKa = 11.84IQPSFQSKK834 pKa = 9.32FLLYY838 pKa = 10.68DD839 pKa = 3.89FYY841 pKa = 11.32NTIYY845 pKa = 10.62GGFMQPYY852 pKa = 8.86SSLIVASQTDD862 pKa = 3.6TTKK865 pKa = 10.7CSNGEE870 pKa = 3.86FTRR873 pKa = 11.84LYY875 pKa = 10.43MMVPSTLISNFTTPIIISDD894 pKa = 3.57IDD896 pKa = 3.97LKK898 pKa = 11.23VAASTYY904 pKa = 10.29RR905 pKa = 11.84AIDD908 pKa = 4.63LISRR912 pKa = 11.84APITYY917 pKa = 10.16GSLALTLRR925 pKa = 11.84NIYY928 pKa = 10.51SGTIEE933 pKa = 4.73ANTFICNTPQSTLYY947 pKa = 10.22RR948 pKa = 11.84ATVVLSCNGVCQTSNTTRR966 pKa = 11.84MLSRR970 pKa = 11.84SVTRR974 pKa = 11.84ADD976 pKa = 3.45RR977 pKa = 11.84TIIDD981 pKa = 5.1DD982 pKa = 3.39IAFYY986 pKa = 7.73TTNAYY991 pKa = 7.49YY992 pKa = 9.89TRR994 pKa = 11.84QGLYY998 pKa = 9.96VPSLVGPIEE1007 pKa = 3.81ITPYY1011 pKa = 10.75SSGSCTVGYY1020 pKa = 10.42DD1021 pKa = 3.67SNGGLLMIDD1030 pKa = 3.75YY1031 pKa = 10.45EE1032 pKa = 4.57DD1033 pKa = 4.04VNVEE1037 pKa = 4.05HH1038 pKa = 6.78VDD1040 pKa = 3.62YY1041 pKa = 11.07RR1042 pKa = 11.84IDD1044 pKa = 3.58LQSFKK1049 pKa = 11.07VSYY1052 pKa = 10.52SPNKK1056 pKa = 9.89VFIKK1060 pKa = 9.91PSPSACTGRR1069 pKa = 11.84TFVSDD1074 pKa = 3.28TNALLTCDD1082 pKa = 4.38SDD1084 pKa = 4.32CCLTTFDD1091 pKa = 4.58NYY1093 pKa = 11.32KK1094 pKa = 10.29QFTIMNAGDD1103 pKa = 3.75VVKK1106 pKa = 10.39FPQSSDD1112 pKa = 2.94ALTLTQYY1119 pKa = 10.32FPSGNQRR1126 pKa = 11.84NVFCPASGCSYY1137 pKa = 10.72ATMLDD1142 pKa = 3.72VYY1144 pKa = 11.23NCALKK1149 pKa = 10.27HH1150 pKa = 5.62HH1151 pKa = 6.65AASTVFLFYY1160 pKa = 9.87FVPIFLGVLIVVYY1173 pKa = 9.95LCRR1176 pKa = 11.84QVIKK1180 pKa = 10.83GFLYY1184 pKa = 10.44GFRR1187 pKa = 11.84NLKK1190 pKa = 9.56FLNPAFYY1197 pKa = 10.66ADD1199 pKa = 4.26KK1200 pKa = 10.38IYY1202 pKa = 10.81NPSVAAVSQIRR1213 pKa = 11.84NSTLRR1218 pKa = 11.84RR1219 pKa = 11.84VKK1221 pKa = 10.48RR1222 pKa = 11.84ITSNKK1227 pKa = 9.64DD1228 pKa = 2.62AA1229 pKa = 4.99

Molecular weight: 136.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJS4|A0A1L3KJS4_9VIRU Putative glycoprotein OS=Hubei virga-like virus 23 OX=1923338 PE=4 SV=1
MM1 pKa = 7.03NRR3 pKa = 11.84SVSTQQLDD11 pKa = 3.47RR12 pKa = 11.84FRR14 pKa = 11.84PNARR18 pKa = 11.84RR19 pKa = 11.84GQTRR23 pKa = 11.84RR24 pKa = 11.84SSSLSRR30 pKa = 11.84NDD32 pKa = 2.79NPAYY36 pKa = 9.87YY37 pKa = 9.96KK38 pKa = 10.69PKK40 pKa = 9.71TPPRR44 pKa = 11.84NNVKK48 pKa = 9.7VVYY51 pKa = 10.33LSEE54 pKa = 3.8PTANIKK60 pKa = 9.18TKK62 pKa = 10.48FNGYY66 pKa = 9.27VNYY69 pKa = 9.76PDD71 pKa = 6.43LFIKK75 pKa = 9.49WQDD78 pKa = 3.28LVSIVLRR85 pKa = 11.84TAKK88 pKa = 10.11IFSSKK93 pKa = 8.96LTNKK97 pKa = 10.11DD98 pKa = 3.15FARR101 pKa = 11.84LFTLADD107 pKa = 3.94RR108 pKa = 11.84SIKK111 pKa = 10.67LHH113 pKa = 4.66TTCNYY118 pKa = 9.35VFEE121 pKa = 4.72IQGQSDD127 pKa = 3.2KK128 pKa = 10.75GAKK131 pKa = 9.7VMTIAMHH138 pKa = 6.65GPASANDD145 pKa = 3.67LFINDD150 pKa = 4.3SLNDD154 pKa = 3.72IIAAVEE160 pKa = 4.28LIGAQTIIPQTLSSGKK176 pKa = 10.2FFFTKK181 pKa = 10.48VKK183 pKa = 10.55LNSNSKK189 pKa = 10.11EE190 pKa = 3.9SSIVNDD196 pKa = 3.81KK197 pKa = 10.99NSPSLFSDD205 pKa = 4.23TIKK208 pKa = 10.88LGLSSGSSKK217 pKa = 11.03SS218 pKa = 3.32

Molecular weight: 24.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4680

136

2702

780.0

87.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.641 ± 0.463

2.521 ± 0.21

5.726 ± 0.477

4.081 ± 0.993

4.487 ± 0.342

4.252 ± 0.288

2.244 ± 0.564

7.692 ± 0.543

5.62 ± 0.949

8.611 ± 0.441

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.902 ± 0.268

5.897 ± 0.473

4.444 ± 0.521

3.12 ± 0.254

4.252 ± 0.213

8.974 ± 0.874

7.436 ± 0.944

7.415 ± 0.717

0.577 ± 0.164

5.064 ± 0.635
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski