Glycomyces buryatensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Glycomycetales; Glycomycetaceae; Glycomyces

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5187 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S8QEG2|A0A4S8QEG2_9ACTN Uncharacterized protein OS=Glycomyces buryatensis OX=2570927 GN=FAB82_03245 PE=4 SV=1
MM1 pKa = 6.89QVHH4 pKa = 6.52RR5 pKa = 11.84SRR7 pKa = 11.84RR8 pKa = 11.84KK9 pKa = 9.51LIVGVAVASLAASAVTLASANSAFAEE35 pKa = 4.53EE36 pKa = 5.27GCQVDD41 pKa = 4.02YY42 pKa = 11.46NITSSWNGGFQANVVISADD61 pKa = 3.53EE62 pKa = 5.08AINGWDD68 pKa = 3.75VSWDD72 pKa = 3.94FPSGTSVASAWNVDD86 pKa = 3.08WSQSGTTFTGSDD98 pKa = 3.62VGWNASISSGQSRR111 pKa = 11.84EE112 pKa = 3.73VFGFIGSGSSTAPAEE127 pKa = 3.99FTLNGDD133 pKa = 3.61VCDD136 pKa = 4.89GPGTGPTEE144 pKa = 4.49PPTSDD149 pKa = 3.64EE150 pKa = 4.32PTDD153 pKa = 4.04EE154 pKa = 4.49PTDD157 pKa = 4.05PPDD160 pKa = 3.64GSRR163 pKa = 11.84VDD165 pKa = 3.56NPYY168 pKa = 10.68VGAQVYY174 pKa = 8.76VNPTWAANAEE184 pKa = 4.2ASGGEE189 pKa = 4.06AVADD193 pKa = 3.68QPTGVWMDD201 pKa = 4.62RR202 pKa = 11.84ISAIEE207 pKa = 4.44GNDD210 pKa = 3.47SPTTGSMGMRR220 pKa = 11.84DD221 pKa = 4.1HH222 pKa = 7.43LDD224 pKa = 3.28AALTQGADD232 pKa = 4.09LIQFVIYY239 pKa = 10.0NLPGRR244 pKa = 11.84DD245 pKa = 3.5CAALASNGEE254 pKa = 4.21LGAGEE259 pKa = 3.65IDD261 pKa = 3.55RR262 pKa = 11.84YY263 pKa = 9.4KK264 pKa = 11.01NEE266 pKa = 4.36YY267 pKa = 9.03IDD269 pKa = 5.2PIVEE273 pKa = 4.15IQSDD277 pKa = 3.71PAYY280 pKa = 10.81ADD282 pKa = 3.04IKK284 pKa = 9.73IVNVIEE290 pKa = 4.5IDD292 pKa = 3.68SLPNLVTNVSPRR304 pKa = 11.84EE305 pKa = 3.97TATTNCDD312 pKa = 2.82TMLANGNYY320 pKa = 9.54EE321 pKa = 4.46DD322 pKa = 4.12GVSYY326 pKa = 10.74AVSEE330 pKa = 5.36LGDD333 pKa = 3.5QPNSYY338 pKa = 10.55QYY340 pKa = 11.47LDD342 pKa = 4.11AGHH345 pKa = 6.97HH346 pKa = 5.78GWIGWGDD353 pKa = 3.68TNAMYY358 pKa = 11.1DD359 pKa = 3.58NFFASADD366 pKa = 3.78LFGSLIGQNGMTANDD381 pKa = 3.23VSGFITNTANYY392 pKa = 9.28SALEE396 pKa = 3.94EE397 pKa = 4.81PYY399 pKa = 10.61WNVDD403 pKa = 3.66DD404 pKa = 5.05NVGGQAINQNPDD416 pKa = 3.45VKK418 pKa = 10.42WVDD421 pKa = 3.06WNDD424 pKa = 3.45FNGEE428 pKa = 3.98LDD430 pKa = 3.58FAQAFRR436 pKa = 11.84TEE438 pKa = 4.35LVSAGYY444 pKa = 10.63NDD446 pKa = 5.63DD447 pKa = 3.51IGMLIDD453 pKa = 3.64TSRR456 pKa = 11.84NGWGGDD462 pKa = 3.42YY463 pKa = 10.62RR464 pKa = 11.84PTGTSTSTDD473 pKa = 2.82PSTYY477 pKa = 9.87VDD479 pKa = 3.84EE480 pKa = 4.64SRR482 pKa = 11.84IDD484 pKa = 3.27QRR486 pKa = 11.84YY487 pKa = 8.56QKK489 pKa = 11.16GNWCNQAGAGLGEE502 pKa = 4.45RR503 pKa = 11.84PVAAPEE509 pKa = 4.15PGIDD513 pKa = 3.05AYY515 pKa = 11.48VWIKK519 pKa = 10.65PPGEE523 pKa = 3.84SDD525 pKa = 3.57GASEE529 pKa = 6.26LIDD532 pKa = 3.66NDD534 pKa = 3.32EE535 pKa = 4.37GKK537 pKa = 10.97GFDD540 pKa = 3.97EE541 pKa = 4.69MCC543 pKa = 4.8

Molecular weight: 57.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V4HQV1|A0A4V4HQV1_9ACTN Amidohydrolase OS=Glycomyces buryatensis OX=2570927 GN=FAB82_23520 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.7LLRR22 pKa = 11.84KK23 pKa = 7.95TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5187

0

5187

1676623

29

1801

323.2

34.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.87 ± 0.048

0.783 ± 0.01

6.464 ± 0.032

6.546 ± 0.038

3.103 ± 0.018

8.926 ± 0.032

2.111 ± 0.016

4.259 ± 0.024

2.311 ± 0.023

9.757 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.956 ± 0.015

2.279 ± 0.021

5.461 ± 0.032

2.881 ± 0.018

7.083 ± 0.041

5.508 ± 0.023

5.905 ± 0.025

7.816 ± 0.029

1.707 ± 0.016

2.274 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski