Neptunomonas concharum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Neptunomonas

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3270 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P1RE62|A0A5P1RE62_9GAMM DUF3306 domain-containing protein OS=Neptunomonas concharum OX=1031538 GN=F0U83_15000 PE=4 SV=1
MM1 pKa = 7.39TEE3 pKa = 4.22TVASSAPMVFTDD15 pKa = 4.07SAAAKK20 pKa = 9.21VKK22 pKa = 10.76SLIEE26 pKa = 3.94EE27 pKa = 4.17EE28 pKa = 4.31QNDD31 pKa = 3.89NLKK34 pKa = 10.76LRR36 pKa = 11.84VFITGGGCAGFSYY49 pKa = 10.74GFTFDD54 pKa = 4.12EE55 pKa = 4.96EE56 pKa = 4.41VAEE59 pKa = 5.65DD60 pKa = 3.55DD61 pKa = 4.27TQIEE65 pKa = 4.35NQGVLMVVDD74 pKa = 4.41PMSFQYY80 pKa = 11.17LEE82 pKa = 4.26GSEE85 pKa = 3.94VDD87 pKa = 3.64YY88 pKa = 11.67TEE90 pKa = 4.91GLQGSQFKK98 pKa = 10.67INNPNATTTCGCGSSFSII116 pKa = 5.02

Molecular weight: 12.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P1RFG5|A0A5P1RFG5_9GAMM AAA family ATPase OS=Neptunomonas concharum OX=1031538 GN=F0U83_08215 PE=4 SV=1
MM1 pKa = 7.43GFCSASKK8 pKa = 10.22RR9 pKa = 11.84SASKK13 pKa = 9.99RR14 pKa = 11.84SASRR18 pKa = 11.84RR19 pKa = 11.84SASRR23 pKa = 11.84RR24 pKa = 11.84SASRR28 pKa = 11.84RR29 pKa = 11.84SASRR33 pKa = 11.84RR34 pKa = 11.84SASRR38 pKa = 11.84RR39 pKa = 11.84SASRR43 pKa = 11.84RR44 pKa = 11.84SASRR48 pKa = 11.84RR49 pKa = 11.84SASKK53 pKa = 9.97RR54 pKa = 11.84SASKK58 pKa = 10.19RR59 pKa = 11.84SASKK63 pKa = 10.19RR64 pKa = 11.84SASKK68 pKa = 10.19RR69 pKa = 11.84SASKK73 pKa = 10.19RR74 pKa = 11.84SASKK78 pKa = 10.19RR79 pKa = 11.84SASKK83 pKa = 10.19RR84 pKa = 11.84SASKK88 pKa = 10.19RR89 pKa = 11.84SASKK93 pKa = 10.19RR94 pKa = 11.84SASKK98 pKa = 10.19RR99 pKa = 11.84SASKK103 pKa = 10.19RR104 pKa = 11.84SASKK108 pKa = 10.19RR109 pKa = 11.84SASKK113 pKa = 10.19RR114 pKa = 11.84SASKK118 pKa = 10.19RR119 pKa = 11.84SASKK123 pKa = 10.19RR124 pKa = 11.84SASKK128 pKa = 10.19RR129 pKa = 11.84SASKK133 pKa = 9.99RR134 pKa = 11.84SASRR138 pKa = 11.84RR139 pKa = 11.84SASKK143 pKa = 9.97RR144 pKa = 11.84SASKK148 pKa = 9.99RR149 pKa = 11.84SASRR153 pKa = 11.84RR154 pKa = 11.84SASKK158 pKa = 10.38RR159 pKa = 11.84SASACSARR167 pKa = 3.92

Molecular weight: 17.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3270

0

3270

1078637

23

8049

329.9

36.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.935 ± 0.037

1.036 ± 0.016

5.513 ± 0.053

6.472 ± 0.039

3.893 ± 0.031

7.011 ± 0.04

2.324 ± 0.026

6.215 ± 0.032

4.719 ± 0.054

10.835 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.583 ± 0.03

3.66 ± 0.026

4.231 ± 0.027

4.511 ± 0.036

5.141 ± 0.051

6.534 ± 0.037

5.327 ± 0.108

6.954 ± 0.057

1.238 ± 0.02

2.867 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski