Saccharomonospora sp. CUA-673

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Saccharomonospora; unclassified Saccharomonospora

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4089 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q9V6B6|A0A1Q9V6B6_9PSEU Nucleotide exchange factor GrpE OS=Saccharomonospora sp. CUA-673 OX=1904969 GN=BJF85_18665 PE=4 SV=1
MM1 pKa = 7.62AGLVLTACGTGTEE14 pKa = 4.87GEE16 pKa = 4.38ATPDD20 pKa = 4.04DD21 pKa = 4.47DD22 pKa = 5.37TPATPAAEE30 pKa = 4.07SSPAGGNGAGEE41 pKa = 4.65VPRR44 pKa = 11.84VPNPVDD50 pKa = 3.59VAPLEE55 pKa = 4.07QDD57 pKa = 2.87PCAALDD63 pKa = 3.79DD64 pKa = 4.11QQIDD68 pKa = 3.99EE69 pKa = 5.16LNLEE73 pKa = 4.38AGQPEE78 pKa = 4.89EE79 pKa = 4.34LTDD82 pKa = 4.09GEE84 pKa = 4.81WICKK88 pKa = 9.0YY89 pKa = 10.43RR90 pKa = 11.84YY91 pKa = 9.51AADD94 pKa = 3.67TNTVVSFVQSPTLTNGLSDD113 pKa = 3.54VYY115 pKa = 10.88DD116 pKa = 3.82RR117 pKa = 11.84KK118 pKa = 10.74DD119 pKa = 3.11AVVVFEE125 pKa = 4.23PTDD128 pKa = 3.73VAGHH132 pKa = 6.3PAVVTQTHH140 pKa = 6.73RR141 pKa = 11.84DD142 pKa = 3.41SRR144 pKa = 11.84DD145 pKa = 2.97QGYY148 pKa = 10.94CDD150 pKa = 4.11LQVGLTDD157 pKa = 3.58SDD159 pKa = 4.28VVTVVAQIAEE169 pKa = 4.4SNDD172 pKa = 3.95DD173 pKa = 3.89YY174 pKa = 11.67PQGCAVAEE182 pKa = 4.33VFAEE186 pKa = 3.94EE187 pKa = 4.25MIEE190 pKa = 3.92NLRR193 pKa = 11.84GGAA196 pKa = 3.58

Molecular weight: 20.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q9VE16|A0A1Q9VE16_9PSEU Assimilatory sulfite reductase (ferredoxin) OS=Saccharomonospora sp. CUA-673 OX=1904969 GN=BJF85_13155 PE=4 SV=1
MM1 pKa = 6.63TTSSPTTRR9 pKa = 11.84RR10 pKa = 11.84ATGTGRR16 pKa = 11.84SSWPRR21 pKa = 11.84CSTSAPEE28 pKa = 4.01ASAMSARR35 pKa = 11.84SFTASSAPRR44 pKa = 11.84AAQTSARR51 pKa = 11.84RR52 pKa = 11.84SSAASSSRR60 pKa = 11.84ASSAGFSRR68 pKa = 11.84SWTMSTPPASAASTNSTRR86 pKa = 11.84SGRR89 pKa = 11.84IGVHH93 pKa = 5.99RR94 pKa = 11.84YY95 pKa = 8.69NRR97 pKa = 11.84ASARR101 pKa = 11.84RR102 pKa = 11.84VRR104 pKa = 11.84II105 pKa = 3.6

Molecular weight: 10.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4089

0

4089

1144911

37

1544

280.0

29.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.085 ± 0.052

0.788 ± 0.011

6.604 ± 0.038

5.963 ± 0.04

2.69 ± 0.022

9.343 ± 0.04

2.271 ± 0.018

3.438 ± 0.028

1.865 ± 0.026

9.9 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.878 ± 0.015

1.866 ± 0.02

5.808 ± 0.036

2.86 ± 0.026

7.835 ± 0.045

5.224 ± 0.028

6.245 ± 0.031

8.923 ± 0.039

1.479 ± 0.017

1.934 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski