Rhodopirellula sp. SM50

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Rhodopirellula; unclassified Rhodopirellula

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6414 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A2W7E3|A0A2A2W7E3_9BACT Peptidase_A24 domain-containing protein OS=Rhodopirellula sp. SM50 OX=595453 GN=CKO51_23740 PE=3 SV=1
MM1 pKa = 7.1NVSRR5 pKa = 11.84QCLFVLFAAGSLCSMAGAEE24 pKa = 4.11TVTFEE29 pKa = 4.66NLLTTPEE36 pKa = 4.05SFYY39 pKa = 11.23KK40 pKa = 10.84GDD42 pKa = 3.66LSQINSDD49 pKa = 3.95PLTVDD54 pKa = 3.02GFEE57 pKa = 4.94FSNQTTYY64 pKa = 11.08VDD66 pKa = 3.94PVTLDD71 pKa = 4.24GYY73 pKa = 9.38WSGWAYY79 pKa = 11.51SNTTDD84 pKa = 2.97TTTPGFANEE93 pKa = 3.91QSAITGGGSNGAGGSVSGEE112 pKa = 4.04TYY114 pKa = 11.22ALAFGSGATINLPAGALIEE133 pKa = 4.5SVDD136 pKa = 3.92WTNGTYY142 pKa = 9.96PYY144 pKa = 11.22LSMRR148 pKa = 11.84DD149 pKa = 2.74GDD151 pKa = 4.36AFAKK155 pKa = 10.28QFGGAGGSEE164 pKa = 3.69PDD166 pKa = 3.73FFRR169 pKa = 11.84VILTGYY175 pKa = 10.31SDD177 pKa = 4.96LNATGTATGSVTLDD191 pKa = 3.19LADD194 pKa = 3.63YY195 pKa = 8.39TFSDD199 pKa = 4.26DD200 pKa = 3.7NQDD203 pKa = 4.03YY204 pKa = 10.84IVDD207 pKa = 3.68AWQVNEE213 pKa = 5.05DD214 pKa = 3.57LTALGNARR222 pKa = 11.84SIDD225 pKa = 3.89LVFQSSDD232 pKa = 2.94SGQFGINTPTYY243 pKa = 10.94LFIDD247 pKa = 3.56NLRR250 pKa = 11.84YY251 pKa = 9.81SVTAIPEE258 pKa = 3.79PTAFGFLALVGGVFTLRR275 pKa = 11.84RR276 pKa = 11.84RR277 pKa = 11.84RR278 pKa = 3.51

Molecular weight: 29.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A2WIU8|A0A2A2WIU8_9BACT Stage V sporulation protein G OS=Rhodopirellula sp. SM50 OX=595453 GN=CKO51_02225 PE=4 SV=1
MM1 pKa = 7.5RR2 pKa = 11.84CEE4 pKa = 3.64KK5 pKa = 10.38RR6 pKa = 11.84SRR8 pKa = 11.84KK9 pKa = 10.18LGDD12 pKa = 3.31TVGVISRR19 pKa = 11.84WRR21 pKa = 11.84FIRR24 pKa = 11.84TAVKK28 pKa = 10.29APRR31 pKa = 11.84SEE33 pKa = 4.13PDD35 pKa = 3.39TIVFGSQDD43 pKa = 2.85RR44 pKa = 11.84TALMRR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84CLRR54 pKa = 11.84DD55 pKa = 3.39LEE57 pKa = 4.39YY58 pKa = 10.49PPLYY62 pKa = 10.16RR63 pKa = 11.84GEE65 pKa = 4.29VVDD68 pKa = 4.56

Molecular weight: 8.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6414

0

6414

2788521

35

13982

434.8

47.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.856 ± 0.042

1.031 ± 0.021

6.9 ± 0.053

5.816 ± 0.031

3.664 ± 0.02

7.995 ± 0.056

2.15 ± 0.021

5.031 ± 0.017

3.145 ± 0.039

9.529 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.048 ± 0.025

3.147 ± 0.033

5.214 ± 0.025

4.092 ± 0.022

6.755 ± 0.051

6.752 ± 0.037

5.855 ± 0.056

7.268 ± 0.03

1.431 ± 0.015

2.319 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski