Streptomyces sp. C

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7528 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9VNP3|D9VNP3_9ACTN DUF202 domain-containing protein OS=Streptomyces sp. C OX=253839 GN=SSNG_01523 PE=4 SV=1
MM1 pKa = 6.63QQEE4 pKa = 4.42APTGGSDD11 pKa = 3.8GAGEE15 pKa = 4.07PLEE18 pKa = 4.18VWIDD22 pKa = 3.44QDD24 pKa = 3.99LCTGDD29 pKa = 4.65GICVQYY35 pKa = 10.96APEE38 pKa = 4.18VFEE41 pKa = 5.9LDD43 pKa = 3.05IDD45 pKa = 3.63GLAYY49 pKa = 10.42VKK51 pKa = 10.8SADD54 pKa = 4.67DD55 pKa = 3.99EE56 pKa = 4.47LLQEE60 pKa = 4.88AGATTPVPLVLLQDD74 pKa = 3.91VVDD77 pKa = 4.33SAKK80 pKa = 10.25EE81 pKa = 3.92CPGDD85 pKa = 4.18CIHH88 pKa = 6.36VRR90 pKa = 11.84RR91 pKa = 11.84VSDD94 pKa = 3.3RR95 pKa = 11.84VEE97 pKa = 4.16VYY99 pKa = 10.89GPDD102 pKa = 3.45AEE104 pKa = 4.31

Molecular weight: 11.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9W321|D9W321_9ACTN Uncharacterized protein (Fragment) OS=Streptomyces sp. C OX=253839 GN=SSNG_05547 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.86GRR40 pKa = 11.84SALSAA45 pKa = 3.8

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7528

0

7528

2294570

29

4488

304.8

32.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.087 ± 0.047

0.846 ± 0.008

5.652 ± 0.02

5.697 ± 0.028

2.708 ± 0.017

9.798 ± 0.034

2.311 ± 0.014

2.947 ± 0.022

2.142 ± 0.021

10.246 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.756 ± 0.012

1.715 ± 0.014

6.485 ± 0.031

2.67 ± 0.016

8.344 ± 0.038

4.889 ± 0.021

5.902 ± 0.024

8.232 ± 0.027

1.539 ± 0.012

2.033 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski