Chlamydiales bacterium SCGC AG-110-P3

Taxonomy: cellular organisms; Bacteria; PVC group; Chlamydiae; Chlamydiia; Chlamydiales; unclassified Chalmydiales

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1225 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A212KVQ9|A0A212KVQ9_9CHLA Uncharacterized protein OS=Chlamydiales bacterium SCGC AG-110-P3 OX=1871323 GN=SCG7086_BH_00090 PE=4 SV=1
MM1 pKa = 7.16YY2 pKa = 10.46LLRR5 pKa = 11.84VLILFIIGFSGSLLSVEE22 pKa = 4.36AAADD26 pKa = 3.59VGGQPASGFLLNKK39 pKa = 7.92PTASGQGVVHH49 pKa = 6.79GNMFPYY55 pKa = 9.92TIYY58 pKa = 10.75YY59 pKa = 10.31DD60 pKa = 3.62PAKK63 pKa = 10.33WEE65 pKa = 4.12MFAGNPGDD73 pKa = 3.48EE74 pKa = 4.22RR75 pKa = 11.84EE76 pKa = 4.37YY77 pKa = 10.87TFSPVDD83 pKa = 3.61GSQAQVWVEE92 pKa = 3.74FRR94 pKa = 11.84SEE96 pKa = 3.88RR97 pKa = 11.84VHH99 pKa = 6.15YY100 pKa = 10.5QEE102 pKa = 6.04LPQNLIDD109 pKa = 4.56EE110 pKa = 4.61VTADD114 pKa = 3.99GLSDD118 pKa = 3.55VQIEE122 pKa = 4.37LFEE125 pKa = 4.04MLEE128 pKa = 4.37VNGKK132 pKa = 9.45YY133 pKa = 10.63YY134 pKa = 10.35IFIHH138 pKa = 5.26MTADD142 pKa = 3.04TGDD145 pKa = 3.72DD146 pKa = 4.1DD147 pKa = 6.08KK148 pKa = 12.12LMGYY152 pKa = 9.22IFHH155 pKa = 7.04YY156 pKa = 9.76LTDD159 pKa = 3.69AGMLRR164 pKa = 11.84LAFVTLEE171 pKa = 4.24SEE173 pKa = 4.8GDD175 pKa = 3.48VHH177 pKa = 7.62DD178 pKa = 5.93QDD180 pKa = 4.78FQDD183 pKa = 4.55LLFSTEE189 pKa = 4.03LQDD192 pKa = 4.12

Molecular weight:
21.6 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A212KWQ3|A0A212KWQ3_9CHLA Uncharacterized protein (Fragment) OS=Chlamydiales bacterium SCGC AG-110-P3 OX=1871323 GN=SCG7086_AK_00010 PE=4 SV=1
MM1 pKa = 7.7CEE3 pKa = 3.83LVEE6 pKa = 4.2LRR8 pKa = 11.84VVRR11 pKa = 11.84LKK13 pKa = 10.53RR14 pKa = 11.84VRR16 pKa = 11.84VGGAHH21 pKa = 7.06LGEE24 pKa = 4.41LPEE27 pKa = 4.11RR28 pKa = 11.84HH29 pKa = 5.46WRR31 pKa = 11.84HH32 pKa = 5.6LSKK35 pKa = 10.73EE36 pKa = 4.01ATNCGTFRR44 pKa = 11.84RR45 pKa = 11.84GLKK48 pKa = 9.81IGNCKK53 pKa = 10.17NYY55 pKa = 10.0RR56 pKa = 11.84NRR58 pKa = 11.84RR59 pKa = 11.84GSFQGNTSGGRR70 pKa = 11.84EE71 pKa = 3.98HH72 pKa = 7.99SKK74 pKa = 8.12MTLLCEE80 pKa = 3.94GHH82 pKa = 6.55RR83 pKa = 11.84LVFGVRR89 pKa = 11.84KK90 pKa = 9.14LAFAFFSSRR99 pKa = 11.84LTGVPCGYY107 pKa = 10.55LRR109 pKa = 11.84ILKK112 pKa = 9.75RR113 pKa = 11.84DD114 pKa = 3.31SNAFFLPQVRR124 pKa = 11.84SLDD127 pKa = 3.82GLEE130 pKa = 4.16SSHH133 pKa = 7.23LFFYY137 pKa = 11.04LEE139 pKa = 3.98QLTGPKK145 pKa = 10.14GRR147 pKa = 11.84QQAGYY152 pKa = 10.21

Molecular weight:
17.34 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1225

0

1225

363987

30

3963

297.1

33.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.135 ± 0.074

1.327 ± 0.031

5.636 ± 0.053

6.663 ± 0.073

3.859 ± 0.047

6.824 ± 0.068

2.31 ± 0.038

6.255 ± 0.064

5.184 ± 0.09

10.275 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.339 ± 0.034

3.667 ± 0.053

4.194 ± 0.044

3.969 ± 0.06

5.808 ± 0.06

6.958 ± 0.073

5.805 ± 0.055

6.754 ± 0.065

1.174 ± 0.026

2.858 ± 0.043

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski