Escherichia phage SRT8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tunavirinae; Sertoctavirus; Escherichia virus SRT8

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1GPC9|A0A2D1GPC9_9CAUD TtpM OS=Escherichia phage SRT8 OX=2496545 PE=4 SV=1
MM1 pKa = 7.15EE2 pKa = 5.43QDD4 pKa = 3.9NFWTRR9 pKa = 11.84YY10 pKa = 6.11FTALYY15 pKa = 10.43AGLNRR20 pKa = 11.84EE21 pKa = 4.09FCLKK25 pKa = 10.13VAYY28 pKa = 9.69KK29 pKa = 10.55EE30 pKa = 4.12MSLDD34 pKa = 3.56EE35 pKa = 4.91ALGDD39 pKa = 3.53MNMDD43 pKa = 4.43DD44 pKa = 4.7EE45 pKa = 6.21SEE47 pKa = 4.02FDD49 pKa = 3.85PNFVMTAEE57 pKa = 4.81DD58 pKa = 3.48NSIPGKK64 pKa = 10.45DD65 pKa = 3.65YY66 pKa = 11.19IPWW69 pKa = 4.04

Molecular weight: 8.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1GP95|A0A2D1GP95_9CAUD Uncharacterized protein OS=Escherichia phage SRT8 OX=2496545 PE=4 SV=1
MM1 pKa = 7.52TNQNVNEE8 pKa = 4.11WKK10 pKa = 9.72VRR12 pKa = 11.84GMISWQGAEE21 pKa = 3.99AAHH24 pKa = 6.1RR25 pKa = 11.84AYY27 pKa = 10.34RR28 pKa = 11.84RR29 pKa = 11.84QGCKK33 pKa = 10.01GYY35 pKa = 9.73RR36 pKa = 11.84ASWLRR41 pKa = 11.84FDD43 pKa = 5.43IKK45 pKa = 10.68FDD47 pKa = 4.41SNGKK51 pKa = 9.33CLEE54 pKa = 4.13KK55 pKa = 10.33RR56 pKa = 11.84CKK58 pKa = 9.4NRR60 pKa = 11.84KK61 pKa = 7.53FMRR64 pKa = 11.84VVRR67 pKa = 11.84KK68 pKa = 10.44DD69 pKa = 3.1EE70 pKa = 4.33TTQSTNSS77 pKa = 3.3

Molecular weight: 9.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

14871

38

1141

177.0

19.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.587 ± 0.439

1.278 ± 0.161

5.85 ± 0.195

7.047 ± 0.285

4.176 ± 0.186

7.78 ± 0.278

1.997 ± 0.23

6.254 ± 0.228

6.738 ± 0.292

6.772 ± 0.217

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.167 ± 0.164

4.835 ± 0.266

3.308 ± 0.212

3.806 ± 0.3

5.124 ± 0.199

5.696 ± 0.2

5.393 ± 0.2

7.108 ± 0.269

1.392 ± 0.119

3.685 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski