Alkalilimnicola ehrlichii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Alkalilimnicola

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3993 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3E0X1A7|A0A3E0X1A7_9GAMM Phage_base_V domain-containing protein OS=Alkalilimnicola ehrlichii OX=351052 GN=CAL65_00885 PE=4 SV=1
MM1 pKa = 7.42SEE3 pKa = 3.78AAVEE7 pKa = 4.01QFTNVIFTDD16 pKa = 3.58SAANKK21 pKa = 8.86VRR23 pKa = 11.84SLIEE27 pKa = 3.77EE28 pKa = 4.2EE29 pKa = 4.34QNDD32 pKa = 3.97DD33 pKa = 3.43LKK35 pKa = 11.4LRR37 pKa = 11.84VYY39 pKa = 9.22ITGGGCSGFQYY50 pKa = 11.01GFTFDD55 pKa = 4.47EE56 pKa = 4.37NVEE59 pKa = 4.43DD60 pKa = 4.95GDD62 pKa = 4.69TIVEE66 pKa = 4.37KK67 pKa = 11.24NGVTLLIDD75 pKa = 3.91PMSGQYY81 pKa = 11.45LMGAEE86 pKa = 3.8IDD88 pKa = 3.87YY89 pKa = 11.31VEE91 pKa = 4.45GVEE94 pKa = 4.72GEE96 pKa = 4.13QFVIRR101 pKa = 11.84NPNASTTCGCGSSFSVV117 pKa = 3.54

Molecular weight: 12.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3E0X258|A0A3E0X258_9GAMM Methyl-accepting transducer domain-containing protein OS=Alkalilimnicola ehrlichii OX=351052 GN=CAL65_05990 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.52RR12 pKa = 11.84KK13 pKa = 8.26RR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.45NGRR28 pKa = 11.84KK29 pKa = 8.88VLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.29GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3993

0

3993

1197236

22

5406

299.8

33.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.246 ± 0.047

0.921 ± 0.014

5.542 ± 0.031

6.676 ± 0.039

3.532 ± 0.026

7.933 ± 0.042

2.279 ± 0.018

4.705 ± 0.029

2.651 ± 0.035

11.047 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.137 ± 0.018

2.838 ± 0.025

4.914 ± 0.033

4.025 ± 0.026

7.721 ± 0.035

5.457 ± 0.03

4.894 ± 0.028

7.34 ± 0.038

1.429 ± 0.019

2.715 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski