Enterococcus quebecensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2789 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E5GWT4|A0A1E5GWT4_9ENTE Isoleucine--tRNA ligase OS=Enterococcus quebecensis OX=903983 GN=ileS PE=3 SV=1
MM1 pKa = 7.16TLAKK5 pKa = 9.51IVYY8 pKa = 10.46ASMTGNTEE16 pKa = 3.89EE17 pKa = 4.35IADD20 pKa = 3.75IVAEE24 pKa = 4.03ALEE27 pKa = 4.18NLDD30 pKa = 3.74IEE32 pKa = 4.76VEE34 pKa = 4.24INEE37 pKa = 4.4CTQVDD42 pKa = 3.93PEE44 pKa = 4.54DD45 pKa = 5.36FEE47 pKa = 5.92DD48 pKa = 5.79ADD50 pKa = 3.52ICVIATYY57 pKa = 9.31TYY59 pKa = 11.45GDD61 pKa = 3.83GEE63 pKa = 4.56LPDD66 pKa = 4.96EE67 pKa = 4.39IVDD70 pKa = 4.56FYY72 pKa = 11.88EE73 pKa = 3.98EE74 pKa = 4.08LQEE77 pKa = 5.13IDD79 pKa = 4.83LSGKK83 pKa = 8.5TYY85 pKa = 10.27GVCGSGDD92 pKa = 3.25TFYY95 pKa = 11.56DD96 pKa = 3.99DD97 pKa = 3.53FCKK100 pKa = 10.86SVDD103 pKa = 3.82DD104 pKa = 4.37FDD106 pKa = 5.41AAFTKK111 pKa = 10.4IGASKK116 pKa = 10.57GADD119 pKa = 3.19SVKK122 pKa = 10.29VDD124 pKa = 4.19LAAEE128 pKa = 4.17EE129 pKa = 4.48EE130 pKa = 4.74DD131 pKa = 3.73IQNLEE136 pKa = 3.96AFAKK140 pKa = 10.22KK141 pKa = 9.96LAEE144 pKa = 4.06AVKK147 pKa = 10.65

Molecular weight: 16.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E5GWG6|A0A1E5GWG6_9ENTE Hemolysin OS=Enterococcus quebecensis OX=903983 GN=BCR23_03035 PE=3 SV=1
MM1 pKa = 7.92PKK3 pKa = 10.12QKK5 pKa = 7.54THH7 pKa = 6.73RR8 pKa = 11.84GSAKK12 pKa = 9.69RR13 pKa = 11.84FKK15 pKa = 10.09RR16 pKa = 11.84TGNGGLKK23 pKa = 10.07RR24 pKa = 11.84FRR26 pKa = 11.84AFTSHH31 pKa = 6.94RR32 pKa = 11.84FHH34 pKa = 7.48GKK36 pKa = 6.37TKK38 pKa = 9.57KK39 pKa = 9.43QRR41 pKa = 11.84RR42 pKa = 11.84QLRR45 pKa = 11.84KK46 pKa = 9.84AGMVSAGDD54 pKa = 3.83FKK56 pKa = 11.18RR57 pKa = 11.84IRR59 pKa = 11.84QQLARR64 pKa = 11.84MKK66 pKa = 10.67

Molecular weight: 7.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2789

0

2789

890319

26

3607

319.2

35.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.55 ± 0.048

0.593 ± 0.014

5.388 ± 0.033

7.408 ± 0.058

4.576 ± 0.04

6.535 ± 0.051

1.673 ± 0.019

7.837 ± 0.04

7.535 ± 0.035

9.584 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.576 ± 0.023

5.026 ± 0.038

3.346 ± 0.025

4.025 ± 0.035

3.502 ± 0.034

6.269 ± 0.037

6.1 ± 0.049

6.898 ± 0.04

0.886 ± 0.015

3.693 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski