Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Quintoviricetes; Piccovirales; Parvoviridae; Parvovirinae; Dependoparvovirus; Adeno-associated dependoparvovirus A; Adeno-associated virus - 2

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q89268|REP78_AAV2S Protein Rep78 OS=Adeno-associated virus 2 (isolate Srivastava/1982) OX=648242 GN=Rep78 PE=1 SV=1
MM1 pKa = 7.47AADD4 pKa = 5.48GYY6 pKa = 11.26LPDD9 pKa = 3.96WLEE12 pKa = 4.24DD13 pKa = 3.65TLSEE17 pKa = 6.15GIRR20 pKa = 11.84QWWKK24 pKa = 10.59LKK26 pKa = 10.21PGPPPPKK33 pKa = 8.73PAEE36 pKa = 3.89RR37 pKa = 11.84HH38 pKa = 5.74KK39 pKa = 11.12DD40 pKa = 3.41DD41 pKa = 4.29SRR43 pKa = 11.84GLVLPGYY50 pKa = 10.19KK51 pKa = 10.22YY52 pKa = 10.62LGPFNGLDD60 pKa = 2.91KK61 pKa = 11.47GEE63 pKa = 4.47PVNEE67 pKa = 3.78ADD69 pKa = 4.62AAALEE74 pKa = 4.24HH75 pKa = 7.12DD76 pKa = 3.9KK77 pKa = 11.32AYY79 pKa = 10.75DD80 pKa = 3.58RR81 pKa = 11.84QLDD84 pKa = 3.91SGDD87 pKa = 3.77NPYY90 pKa = 11.12LKK92 pKa = 10.54YY93 pKa = 10.86NHH95 pKa = 7.21ADD97 pKa = 3.17AEE99 pKa = 4.31FQEE102 pKa = 4.46RR103 pKa = 11.84LKK105 pKa = 11.03EE106 pKa = 3.98DD107 pKa = 3.3TSFGGNLGRR116 pKa = 11.84AVFQAKK122 pKa = 9.74KK123 pKa = 10.24RR124 pKa = 11.84VLEE127 pKa = 3.81PLGLVEE133 pKa = 5.13EE134 pKa = 4.6PVKK137 pKa = 9.85TAPGKK142 pKa = 9.96KK143 pKa = 9.36RR144 pKa = 11.84PVEE147 pKa = 3.98HH148 pKa = 6.89SPVEE152 pKa = 4.15PDD154 pKa = 3.16SSSGTGKK161 pKa = 10.46AGQQPARR168 pKa = 11.84KK169 pKa = 8.94RR170 pKa = 11.84LNFGQTGDD178 pKa = 3.98ADD180 pKa = 4.25SVPDD184 pKa = 4.6PQPLGQPPAAPSGLGTNTMATGSGAPMADD213 pKa = 3.37NNEE216 pKa = 4.16GADD219 pKa = 3.78GVGNSSGNWHH229 pKa = 7.23CDD231 pKa = 3.35STWMGDD237 pKa = 3.49RR238 pKa = 11.84VITTSTRR245 pKa = 11.84TWALPTYY252 pKa = 10.3NNHH255 pKa = 6.8LYY257 pKa = 10.25KK258 pKa = 10.6QISSQSGASNDD269 pKa = 3.08NHH271 pKa = 6.16YY272 pKa = 10.71FGYY275 pKa = 7.95STPWGYY281 pKa = 11.2FDD283 pKa = 5.8FNRR286 pKa = 11.84FHH288 pKa = 6.91CHH290 pKa = 5.57FSPRR294 pKa = 11.84DD295 pKa = 3.43WQRR298 pKa = 11.84LINNNWGFRR307 pKa = 11.84PKK309 pKa = 10.42RR310 pKa = 11.84LNFKK314 pKa = 10.31LFNIQVKK321 pKa = 9.66EE322 pKa = 4.06VTQNDD327 pKa = 4.03GTTTIANNLTSTVQVFTDD345 pKa = 3.48SEE347 pKa = 4.49YY348 pKa = 10.74QLPYY352 pKa = 10.78VLGSAHH358 pKa = 6.25QGCLPPFPADD368 pKa = 2.94VFMVPQYY375 pKa = 11.56GYY377 pKa = 8.93LTLNNGSQAVGRR389 pKa = 11.84SSFYY393 pKa = 10.4CLEE396 pKa = 4.08YY397 pKa = 10.65FPSQMLRR404 pKa = 11.84TGNNFTFSYY413 pKa = 9.76TFEE416 pKa = 4.62DD417 pKa = 4.48VPFHH421 pKa = 6.78SSYY424 pKa = 11.56AHH426 pKa = 5.63SQSLDD431 pKa = 2.94RR432 pKa = 11.84LMNPLIDD439 pKa = 3.29QYY441 pKa = 11.66LYY443 pKa = 10.92YY444 pKa = 10.81LSRR447 pKa = 11.84TNTPSGTTTQSRR459 pKa = 11.84LQFSQAGASDD469 pKa = 3.3IRR471 pKa = 11.84DD472 pKa = 3.38QSRR475 pKa = 11.84NWLPGPCYY483 pKa = 9.9RR484 pKa = 11.84QQRR487 pKa = 11.84VSKK490 pKa = 9.9TSADD494 pKa = 3.88NNNSEE499 pKa = 4.28YY500 pKa = 10.88SWTGATKK507 pKa = 9.7YY508 pKa = 10.23HH509 pKa = 6.45LNGRR513 pKa = 11.84DD514 pKa = 3.41SLVNPGPAMASHH526 pKa = 7.23KK527 pKa = 10.69DD528 pKa = 3.53DD529 pKa = 3.69EE530 pKa = 5.0EE531 pKa = 4.99KK532 pKa = 10.71FFPQSGVLIFGKK544 pKa = 10.26QGSEE548 pKa = 3.75KK549 pKa = 10.33TNVDD553 pKa = 3.06IEE555 pKa = 4.42KK556 pKa = 10.88VMITDD561 pKa = 3.44EE562 pKa = 4.28EE563 pKa = 4.63EE564 pKa = 3.81IRR566 pKa = 11.84TTNPVATEE574 pKa = 4.08QYY576 pKa = 11.02GSVSTNLQRR585 pKa = 11.84GNRR588 pKa = 11.84QAATADD594 pKa = 3.69VNTQGVLPGMVWQDD608 pKa = 2.54RR609 pKa = 11.84DD610 pKa = 3.62VYY612 pKa = 10.93LQGPIWAKK620 pKa = 10.14IPHH623 pKa = 6.57TDD625 pKa = 3.17GHH627 pKa = 5.96FHH629 pKa = 7.43PSPLMGGFGLKK640 pKa = 10.12HH641 pKa = 6.61PPPQILIKK649 pKa = 9.58NTPVPANPSTTFSAAKK665 pKa = 8.7FASFITQYY673 pKa = 9.97STGQVSVEE681 pKa = 4.4IEE683 pKa = 3.74WEE685 pKa = 4.1LQKK688 pKa = 11.15EE689 pKa = 4.16NSKK692 pKa = 10.25RR693 pKa = 11.84WNPEE697 pKa = 2.74IQYY700 pKa = 9.56TSNYY704 pKa = 8.24NKK706 pKa = 10.29SVNVDD711 pKa = 3.7FTVDD715 pKa = 3.26TNGVYY720 pKa = 10.21SEE722 pKa = 4.47PRR724 pKa = 11.84PIGTRR729 pKa = 11.84YY730 pKa = 8.08LTRR733 pKa = 11.84NLL735 pKa = 3.55

Molecular weight: 81.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P03132|REP68_AAV2S Protein Rep68 OS=Adeno-associated virus 2 (isolate Srivastava/1982) OX=648242 GN=Rep68 PE=1 SV=1
MM1 pKa = 7.12EE2 pKa = 4.72TQTQYY7 pKa = 10.29LTPSLSDD14 pKa = 3.2SHH16 pKa = 5.79QQPPLVWEE24 pKa = 4.86LIRR27 pKa = 11.84WLQAVAHH34 pKa = 5.29QWQTITRR41 pKa = 11.84APTEE45 pKa = 3.82WVIPRR50 pKa = 11.84EE51 pKa = 3.73IGIAIPHH58 pKa = 6.11GWATEE63 pKa = 4.09SSPPAPEE70 pKa = 5.12PGPCPPTTTTSTNKK84 pKa = 9.57FPANQEE90 pKa = 3.62PRR92 pKa = 11.84TTITTLATAPLGGILTSTDD111 pKa = 2.83STATFHH117 pKa = 7.0HH118 pKa = 6.19VTGKK122 pKa = 10.44DD123 pKa = 3.15SSTTTGDD130 pKa = 3.14SDD132 pKa = 4.04PRR134 pKa = 11.84DD135 pKa = 3.79STSSSLTFKK144 pKa = 10.5SKK146 pKa = 10.04RR147 pKa = 11.84SRR149 pKa = 11.84RR150 pKa = 11.84MTVRR154 pKa = 11.84RR155 pKa = 11.84RR156 pKa = 11.84LPITLPARR164 pKa = 11.84FRR166 pKa = 11.84CLLTRR171 pKa = 11.84STSSRR176 pKa = 11.84TSSARR181 pKa = 11.84RR182 pKa = 11.84IKK184 pKa = 10.47DD185 pKa = 3.01ASRR188 pKa = 11.84RR189 pKa = 11.84SQQTSSWCHH198 pKa = 6.8SMDD201 pKa = 3.28TSPP204 pKa = 5.28

Molecular weight: 22.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

2

8

3936

204

735

492.0

55.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.479 ± 0.414

1.474 ± 0.353

5.589 ± 0.231

5.208 ± 0.542

4.395 ± 0.18

6.428 ± 0.585

2.363 ± 0.09

4.268 ± 0.431

5.716 ± 0.803

6.174 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.956 ± 0.126

5.996 ± 0.553

6.225 ± 0.562

5.488 ± 0.178

4.751 ± 0.345

8.054 ± 0.621

7.342 ± 0.871

6.352 ± 0.555

2.261 ± 0.098

3.481 ± 0.376
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski