Streptomyces phage SF3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M4S3G8|A0A0M4S3G8_9CAUD Phage_Mu_F domain-containing protein OS=Streptomyces phage SF3 OX=1690818 GN=SF3_840 PE=4 SV=1
MM1 pKa = 7.64SDD3 pKa = 4.14EE4 pKa = 4.39FAPDD8 pKa = 3.51CGAVTPEE15 pKa = 4.32EE16 pKa = 4.12EE17 pKa = 4.33CGEE20 pKa = 4.26CEE22 pKa = 4.38SCFEE26 pKa = 4.16ATAEE30 pKa = 4.75GIDD33 pKa = 3.45RR34 pKa = 11.84AVEE37 pKa = 4.24SGSMTASEE45 pKa = 3.93AAEE48 pKa = 3.78AHH50 pKa = 6.57DD51 pKa = 5.19LNGTYY56 pKa = 9.93RR57 pKa = 11.84RR58 pKa = 11.84EE59 pKa = 3.91

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M5M0T6|A0A0M5M0T6_9CAUD Uncharacterized protein OS=Streptomyces phage SF3 OX=1690818 GN=SF3_70 PE=4 SV=1
MM1 pKa = 7.58INFIADD7 pKa = 3.77LTSALYY13 pKa = 10.25AAFCAFVLAAQAMTPLFPYY32 pKa = 10.17HH33 pKa = 6.22VALTLSIFITLAGGLIGWSWRR54 pKa = 11.84RR55 pKa = 11.84SIWRR59 pKa = 11.84LFGRR63 pKa = 11.84KK64 pKa = 7.95

Molecular weight: 7.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

18079

20

1190

200.9

21.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.27 ± 0.332

1.034 ± 0.136

7.019 ± 0.257

5.824 ± 0.362

2.074 ± 0.146

8.817 ± 0.526

2.318 ± 0.236

3.8 ± 0.174

2.965 ± 0.27

8.203 ± 0.268

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.903 ± 0.101

2.295 ± 0.203

6.272 ± 0.292

3.075 ± 0.212

8.236 ± 0.371

4.762 ± 0.229

6.084 ± 0.249

7.65 ± 0.216

1.991 ± 0.127

2.406 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski