Pseudomonas phage phiAH14a

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0VMC3|A0A1B0VMC3_9CAUD Uncharacterized protein OS=Pseudomonas phage phiAH14a OX=1805958 GN=AH14a_p24 PE=4 SV=1
MM1 pKa = 7.44ARR3 pKa = 11.84TYY5 pKa = 10.35EE6 pKa = 4.03YY7 pKa = 8.09WTVKK11 pKa = 10.38DD12 pKa = 4.07GEE14 pKa = 4.79DD15 pKa = 2.95IAVNLTVISFSASKK29 pKa = 11.28GNFSSQAADD38 pKa = 3.22PDD40 pKa = 4.47EE41 pKa = 4.26YY42 pKa = 10.8HH43 pKa = 6.13GHH45 pKa = 6.87CEE47 pKa = 4.45IEE49 pKa = 4.24WEE51 pKa = 4.52SKK53 pKa = 11.02DD54 pKa = 3.31DD55 pKa = 3.66TSFMTEE61 pKa = 3.82SEE63 pKa = 4.23IASMEE68 pKa = 3.74EE69 pKa = 3.82WLVNEE74 pKa = 3.99HH75 pKa = 6.43SEE77 pKa = 4.15YY78 pKa = 11.1LADD81 pKa = 4.05QDD83 pKa = 5.21YY84 pKa = 11.49YY85 pKa = 11.63DD86 pKa = 3.6

Molecular weight: 9.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0VMG1|A0A1B0VMG1_9CAUD Uncharacterized protein OS=Pseudomonas phage phiAH14a OX=1805958 GN=AH14a_p65 PE=4 SV=1
MM1 pKa = 7.38SAAEE5 pKa = 4.02QFWVVIFLALVTVLIAGHH23 pKa = 6.46FIDD26 pKa = 4.55RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 8.71RR31 pKa = 11.84SIEE34 pKa = 3.7EE35 pKa = 3.87FEE37 pKa = 4.09QKK39 pKa = 9.96RR40 pKa = 11.84RR41 pKa = 11.84EE42 pKa = 4.02RR43 pKa = 11.84KK44 pKa = 8.01TEE46 pKa = 3.62VEE48 pKa = 3.83RR49 pKa = 11.84AARR52 pKa = 11.84KK53 pKa = 9.52ALL55 pKa = 3.61

Molecular weight: 6.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

17024

37

1230

179.2

19.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.789 ± 0.479

1.422 ± 0.193

5.821 ± 0.184

6.55 ± 0.316

3.148 ± 0.152

7.883 ± 0.242

1.58 ± 0.182

5.069 ± 0.179

5.263 ± 0.248

8.042 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.496 ± 0.12

3.366 ± 0.206

4.664 ± 0.28

4.429 ± 0.228

6.174 ± 0.301

5.909 ± 0.309

5.663 ± 0.287

6.438 ± 0.196

1.674 ± 0.14

2.62 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski