Mycoplasma amphoriforme A39

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma amphoriforme

Average proteome isoelectric point is 7.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 715 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A292IHZ5|A0A292IHZ5_9MOLU Uncharacterized protein OS=Mycoplasma amphoriforme A39 OX=572419 GN=MAMA39_01700 PE=3 SV=1
MM1 pKa = 7.33AHH3 pKa = 6.59SNNPAVKK10 pKa = 10.25NNNQGYY16 pKa = 10.41DD17 pKa = 3.51GADD20 pKa = 3.25QQNNEE25 pKa = 4.27AVFNDD30 pKa = 3.34LWGDD34 pKa = 3.54SDD36 pKa = 5.28QDD38 pKa = 4.26LEE40 pKa = 4.62LDD42 pKa = 3.82NSGEE46 pKa = 4.0QADD49 pKa = 4.51EE50 pKa = 4.09EE51 pKa = 4.7FEE53 pKa = 4.3LPEE56 pKa = 5.68DD57 pKa = 3.92GSIQIAYY64 pKa = 9.74DD65 pKa = 3.43ADD67 pKa = 3.71GNGYY71 pKa = 9.43YY72 pKa = 9.67ISYY75 pKa = 10.27DD76 pKa = 3.77YY77 pKa = 9.63EE78 pKa = 3.96TGEE81 pKa = 4.42YY82 pKa = 9.11QDD84 pKa = 3.79PWSGDD89 pKa = 3.42VYY91 pKa = 10.96DD92 pKa = 4.95ISALFDD98 pKa = 3.44EE99 pKa = 4.87QGNPFSFFSDD109 pKa = 3.63EE110 pKa = 4.19PNEE113 pKa = 4.08EE114 pKa = 4.2VVSEE118 pKa = 4.42EE119 pKa = 4.01EE120 pKa = 4.21QEE122 pKa = 4.24ASPEE126 pKa = 3.75QAYY129 pKa = 9.21EE130 pKa = 4.02VQAQQGAEE138 pKa = 3.87QVYY141 pKa = 10.49DD142 pKa = 4.38EE143 pKa = 5.03PAVIDD148 pKa = 3.83DD149 pKa = 4.11EE150 pKa = 5.0VYY152 pKa = 10.47EE153 pKa = 4.3RR154 pKa = 11.84QTHH157 pKa = 6.02DD158 pKa = 4.19LEE160 pKa = 5.13KK161 pKa = 10.51LQQEE165 pKa = 4.25QSVTNQGASYY175 pKa = 9.3QQQHH179 pKa = 6.36DD180 pKa = 4.17VPSEE184 pKa = 4.18SNASIEE190 pKa = 4.36QEE192 pKa = 4.35SEE194 pKa = 3.87QQLPPVQEE202 pKa = 4.37TTSLQADD209 pKa = 3.99EE210 pKa = 4.89TLVTDD215 pKa = 4.12PTLDD219 pKa = 4.84DD220 pKa = 4.47YY221 pKa = 11.02YY222 pKa = 11.25QHH224 pKa = 6.61YY225 pKa = 9.6NQVPYY230 pKa = 10.34RR231 pKa = 11.84YY232 pKa = 9.35LDD234 pKa = 3.24QYY236 pKa = 10.3EE237 pKa = 3.97QDD239 pKa = 3.5ATEE242 pKa = 3.8QALPVVDD249 pKa = 5.34HH250 pKa = 5.88VTSDD254 pKa = 3.55PSQFEE259 pKa = 4.44DD260 pKa = 4.43GEE262 pKa = 4.21QLITGLIGNNGYY274 pKa = 9.75QPDD277 pKa = 3.81NKK279 pKa = 9.69TFEE282 pKa = 4.43INAEE286 pKa = 3.81EE287 pKa = 4.24STAVTEE293 pKa = 5.01LEE295 pKa = 3.94PLVINGIEE303 pKa = 4.06NSQQEE308 pKa = 4.31AAVSQDD314 pKa = 3.23QIGLSRR320 pKa = 11.84TDD322 pKa = 3.59EE323 pKa = 4.24IKK325 pKa = 10.5PDD327 pKa = 3.9LVTIPQINEE336 pKa = 3.84STSAPSQPEE345 pKa = 4.35SNVSVQTEE353 pKa = 4.42RR354 pKa = 11.84YY355 pKa = 7.64STVEE359 pKa = 4.02PVSDD363 pKa = 3.72AMVKK367 pKa = 10.19AYY369 pKa = 9.02YY370 pKa = 9.15QQYY373 pKa = 10.65SEE375 pKa = 4.8IASQSHH381 pKa = 5.81QPTQPQEE388 pKa = 3.87QVVDD392 pKa = 3.86SKK394 pKa = 11.68PNVHH398 pKa = 6.87YY399 pKa = 10.7IPISAIDD406 pKa = 3.38SSYY409 pKa = 10.94QQDD412 pKa = 3.77FDD414 pKa = 4.2PQNDD418 pKa = 3.39FHH420 pKa = 7.41KK421 pKa = 10.95FCDD424 pKa = 3.84EE425 pKa = 3.79QVTAQLHH432 pKa = 4.59PTALVVDD439 pKa = 4.86EE440 pKa = 5.12SNSEE444 pKa = 4.09PEE446 pKa = 4.07DD447 pKa = 3.72EE448 pKa = 4.81KK449 pKa = 11.15PVAVSPVDD457 pKa = 3.11IDD459 pKa = 3.66LAKK462 pKa = 10.86SHH464 pKa = 7.44DD465 pKa = 3.94EE466 pKa = 4.11SSDD469 pKa = 3.53DD470 pKa = 3.5FVLVNEE476 pKa = 4.53PSDD479 pKa = 3.77TKK481 pKa = 11.19EE482 pKa = 3.85LFAKK486 pKa = 10.43KK487 pKa = 9.79SVPEE491 pKa = 3.9NYY493 pKa = 9.76VFEE496 pKa = 4.55NAHH499 pKa = 5.35TQHH502 pKa = 6.1ITPNLVYY509 pKa = 9.98QAEE512 pKa = 4.47SNEE515 pKa = 4.04PSRR518 pKa = 11.84ASQDD522 pKa = 3.43DD523 pKa = 4.59DD524 pKa = 3.91IVDD527 pKa = 4.11SKK529 pKa = 9.08TQPIAIVDD537 pKa = 3.45HH538 pKa = 6.5SEE540 pKa = 4.51NYY542 pKa = 8.83VQPDD546 pKa = 3.39SFLAPISHH554 pKa = 6.59EE555 pKa = 3.62PMLNNEE561 pKa = 4.1NFSYY565 pKa = 10.12TSEE568 pKa = 4.31VADD571 pKa = 3.5QLALPEE577 pKa = 4.36IVPEE581 pKa = 4.49IISATEE587 pKa = 4.02SVSATTAFEE596 pKa = 4.14FANDD600 pKa = 3.45SSKK603 pKa = 9.23QTSQEE608 pKa = 4.1EE609 pKa = 4.74VSVEE613 pKa = 3.95PPVSQVEE620 pKa = 4.34PLTVDD625 pKa = 4.82DD626 pKa = 4.9IFVPLKK632 pKa = 10.49PEE634 pKa = 3.89MEE636 pKa = 4.29FVPDD640 pKa = 4.32KK641 pKa = 11.14VIEE644 pKa = 4.68DD645 pKa = 3.59DD646 pKa = 4.95HH647 pKa = 8.55GCDD650 pKa = 3.8DD651 pKa = 4.1CHH653 pKa = 7.19DD654 pKa = 3.73AHH656 pKa = 6.19EE657 pKa = 4.82TIALFDD663 pKa = 3.85EE664 pKa = 4.95KK665 pKa = 10.59KK666 pKa = 10.86DD667 pKa = 3.5PVEE670 pKa = 5.14AVDD673 pKa = 3.88TMPKK677 pKa = 9.41TEE679 pKa = 4.16TNEE682 pKa = 4.0PEE684 pKa = 3.98EE685 pKa = 4.17QQVDD689 pKa = 3.74EE690 pKa = 5.64AFIPLNFDD698 pKa = 3.21HH699 pKa = 6.88QNLVEE704 pKa = 5.87DD705 pKa = 5.17DD706 pKa = 3.97NQLDD710 pKa = 3.89FTIHH714 pKa = 7.35AEE716 pKa = 4.07EE717 pKa = 4.63QPTVDD722 pKa = 2.71QFVIVEE728 pKa = 4.24EE729 pKa = 4.24NGALQASFATTFAKK743 pKa = 10.36KK744 pKa = 10.27DD745 pKa = 3.66VVCNEE750 pKa = 4.03PEE752 pKa = 4.26GMSFTVVDD760 pKa = 3.81EE761 pKa = 4.52LEE763 pKa = 4.06PVVFVKK769 pKa = 10.4PADD772 pKa = 4.63RR773 pKa = 11.84IVQPEE778 pKa = 4.09KK779 pKa = 10.89PKK781 pKa = 10.77DD782 pKa = 3.57LEE784 pKa = 4.1LAPVEE789 pKa = 4.26FSEE792 pKa = 5.5PSIDD796 pKa = 3.83DD797 pKa = 3.49VKK799 pKa = 11.42VEE801 pKa = 4.45PEE803 pKa = 3.81PVTWPEE809 pKa = 3.58ITPVITDD816 pKa = 3.32VPNSFEE822 pKa = 4.84SNQSLPWYY830 pKa = 10.0AAVGSNASEE839 pKa = 4.38CDD841 pKa = 3.41QKK843 pKa = 9.84THH845 pKa = 6.97ADD847 pKa = 3.49HH848 pKa = 7.02FNEE851 pKa = 3.86VLLNEE856 pKa = 4.52SLAEE860 pKa = 4.02VQKK863 pKa = 9.65TDD865 pKa = 3.76EE866 pKa = 4.21IMEE869 pKa = 4.5VPQSTPSANKK879 pKa = 8.84QDD881 pKa = 2.92ISIFEE886 pKa = 4.32NYY888 pKa = 8.64GKK890 pKa = 9.92PEE892 pKa = 3.53ITIVNDD898 pKa = 3.94EE899 pKa = 4.06ISATPSHH906 pKa = 6.35SLFGKK911 pKa = 9.65SEE913 pKa = 4.19QPVEE917 pKa = 4.12PYY919 pKa = 11.01LPFTITNDD927 pKa = 2.87SDD929 pKa = 5.07LVVQFNKK936 pKa = 10.2KK937 pKa = 9.68PSLSGFKK944 pKa = 10.06FDD946 pKa = 5.44SIQLEE951 pKa = 4.29EE952 pKa = 4.25QQVSPDD958 pKa = 2.96AHH960 pKa = 6.97LEE962 pKa = 3.85PDD964 pKa = 3.5EE965 pKa = 4.95AVAPVPSVVLEE976 pKa = 4.01PEE978 pKa = 3.84QTKK981 pKa = 10.45EE982 pKa = 3.66IEE984 pKa = 4.01PAPKK988 pKa = 9.27IKK990 pKa = 10.29KK991 pKa = 9.94AFWEE995 pKa = 4.53SYY997 pKa = 9.91IGNFDD1002 pKa = 3.65YY1003 pKa = 11.3GHH1005 pKa = 7.09FDD1007 pKa = 5.77DD1008 pKa = 5.49DD1009 pKa = 5.7NKK1011 pKa = 10.41WVWDD1015 pKa = 3.65GFFYY1019 pKa = 11.06EE1020 pKa = 4.47NGTFVSSRR1028 pKa = 11.84TAPPIAPDD1036 pKa = 4.31PKK1038 pKa = 10.94PMTALNQQEE1047 pKa = 4.67SNPTLVTDD1055 pKa = 3.74FLIKK1059 pKa = 10.21KK1060 pKa = 8.95RR1061 pKa = 11.84EE1062 pKa = 3.9EE1063 pKa = 3.81VMVPVRR1069 pKa = 11.84PVQTQAEE1076 pKa = 4.86LYY1078 pKa = 10.46GNPEE1082 pKa = 4.01YY1083 pKa = 10.47DD1084 pKa = 3.33TDD1086 pKa = 4.52VVMLDD1091 pKa = 5.17DD1092 pKa = 5.05EE1093 pKa = 4.89GTSQALLRR1101 pKa = 11.84ANKK1104 pKa = 9.63AHH1106 pKa = 6.45EE1107 pKa = 5.12AILRR1111 pKa = 11.84PKK1113 pKa = 9.91PAVEE1117 pKa = 4.16DD1118 pKa = 3.49KK1119 pKa = 11.22KK1120 pKa = 10.93PVIEE1124 pKa = 5.22IDD1126 pKa = 4.55DD1127 pKa = 3.69IQEE1130 pKa = 4.01VEE1132 pKa = 4.16NEE1134 pKa = 4.18EE1135 pKa = 4.1IVPKK1139 pKa = 9.01VTKK1142 pKa = 10.21TEE1144 pKa = 4.39TILEE1148 pKa = 4.25PKK1150 pKa = 8.55MQPKK1154 pKa = 10.0KK1155 pKa = 11.01FNFLTNRR1162 pKa = 11.84FTPPSEE1168 pKa = 4.09NLDD1171 pKa = 3.59ALTTEE1176 pKa = 4.49IVSGEE1181 pKa = 4.04PSCLSEE1187 pKa = 5.8DD1188 pKa = 4.06DD1189 pKa = 5.06LFASTTYY1196 pKa = 10.87DD1197 pKa = 3.27NKK1199 pKa = 10.6IKK1201 pKa = 9.52EE1202 pKa = 4.57TLLNMVGFLNKK1213 pKa = 9.98ASAEE1217 pKa = 3.97ATHH1220 pKa = 6.31VYY1222 pKa = 8.58KK1223 pKa = 10.75TIAKK1227 pKa = 8.64EE1228 pKa = 3.69LKK1230 pKa = 10.13IEE1232 pKa = 4.09IKK1234 pKa = 10.5NIKK1237 pKa = 8.83QDD1239 pKa = 3.61NEE1241 pKa = 3.88KK1242 pKa = 10.79LRR1244 pKa = 11.84LEE1246 pKa = 4.22FKK1248 pKa = 10.55EE1249 pKa = 3.76NEE1251 pKa = 3.77NRR1253 pKa = 11.84ISAKK1257 pKa = 10.45KK1258 pKa = 10.1SDD1260 pKa = 4.17LLKK1263 pKa = 10.32TITSDD1268 pKa = 4.11FKK1270 pKa = 11.16QGTNFNTGKK1279 pKa = 10.37DD1280 pKa = 3.75FDD1282 pKa = 4.59SLQHH1286 pKa = 5.43AHH1288 pKa = 7.72DD1289 pKa = 4.36YY1290 pKa = 10.81NKK1292 pKa = 10.58HH1293 pKa = 5.76LNEE1296 pKa = 3.81TMKK1299 pKa = 11.04ANEE1302 pKa = 4.11EE1303 pKa = 3.94RR1304 pKa = 11.84LQVLTNNQVQLKK1316 pKa = 9.23IVYY1319 pKa = 9.3DD1320 pKa = 3.55RR1321 pKa = 11.84RR1322 pKa = 11.84MSKK1325 pKa = 10.27LASDD1329 pKa = 4.39ANRR1332 pKa = 11.84LEE1334 pKa = 4.15NLLSGADD1341 pKa = 3.87YY1342 pKa = 10.58RR1343 pKa = 11.84DD1344 pKa = 3.67HH1345 pKa = 7.55SLDD1348 pKa = 3.63MIEE1351 pKa = 3.94EE1352 pKa = 4.26NYY1354 pKa = 10.72RR1355 pKa = 11.84NLLKK1359 pKa = 11.01AQDD1362 pKa = 3.94DD1363 pKa = 3.83YY1364 pKa = 12.02RR1365 pKa = 11.84NRR1367 pKa = 11.84LNHH1370 pKa = 6.2FVPTTFPNLKK1380 pKa = 9.77NQMLPSTGLFQNDD1393 pKa = 3.02FNEE1396 pKa = 4.28FSDD1399 pKa = 5.6LSALDD1404 pKa = 3.23ATFYY1408 pKa = 11.39SKK1410 pKa = 11.01LATPSFYY1417 pKa = 11.3NNNLPLPFEE1426 pKa = 4.2TQPRR1430 pKa = 11.84LLDD1433 pKa = 3.8NSHH1436 pKa = 6.54SLLGNLDD1443 pKa = 3.15NSYY1446 pKa = 11.65DD1447 pKa = 3.8LFSEE1451 pKa = 4.38KK1452 pKa = 11.03SNFTSDD1458 pKa = 3.27LLKK1461 pKa = 10.63PFNPQGQDD1469 pKa = 2.74SLKK1472 pKa = 11.32AMDD1475 pKa = 4.51RR1476 pKa = 11.84YY1477 pKa = 10.42FNRR1480 pKa = 11.84DD1481 pKa = 3.25FEE1483 pKa = 4.55LLKK1486 pKa = 10.36PDD1488 pKa = 3.54RR1489 pKa = 11.84TTSNLDD1495 pKa = 3.57DD1496 pKa = 5.14LFSDD1500 pKa = 4.69DD1501 pKa = 5.82DD1502 pKa = 4.29ALFSDD1507 pKa = 5.17KK1508 pKa = 11.03YY1509 pKa = 10.76DD1510 pKa = 4.0ANASLTDD1517 pKa = 3.56EE1518 pKa = 5.48LEE1520 pKa = 4.35DD1521 pKa = 3.99LLRR1524 pKa = 11.84RR1525 pKa = 4.0

Molecular weight:
172.07 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A292IJ84|A0A292IJ84_9MOLU Uncharacterized protein OS=Mycoplasma amphoriforme A39 OX=572419 GN=MAMA39_06280 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 9.71PHH4 pKa = 7.48LSTFSTVQWYY14 pKa = 9.58KK15 pKa = 10.91CRR17 pKa = 11.84GTRR20 pKa = 11.84KK21 pKa = 10.11SMYY24 pKa = 10.36LNLFVFRR31 pKa = 11.84SLSQSCNCDD40 pKa = 3.15NNRR43 pKa = 11.84LNLSASQVSSTKK55 pKa = 9.88IQANEE60 pKa = 3.66LSRR63 pKa = 11.84MKK65 pKa = 10.78NRR67 pKa = 11.84LLPSDD72 pKa = 4.24SRR74 pKa = 11.84CSDD77 pKa = 2.97GKK79 pKa = 10.78RR80 pKa = 11.84FLRR83 pKa = 11.84LISSIFLFRR92 pKa = 11.84PSIFRR97 pKa = 11.84SKK99 pKa = 10.49YY100 pKa = 8.18CSKK103 pKa = 10.7RR104 pKa = 11.84SNFSLTSAWSTWILSKK120 pKa = 11.09ASLIFFLNFASTSSIFLYY138 pKa = 10.42NKK140 pKa = 9.25SIRR143 pKa = 11.84WFKK146 pKa = 10.78RR147 pKa = 11.84FSTLVIILLISSILALVLFNSSRR170 pKa = 11.84TIFAYY175 pKa = 9.88SGKK178 pKa = 10.66GIIFSLSINTKK189 pKa = 6.97YY190 pKa = 10.25EE191 pKa = 3.71RR192 pKa = 11.84SGLIFFKK199 pKa = 11.13VLLFCIDD206 pKa = 3.49SYY208 pKa = 11.48LIIIFFGDD216 pKa = 3.53KK217 pKa = 10.37YY218 pKa = 11.47NKK220 pKa = 10.36LSIDD224 pKa = 3.68KK225 pKa = 10.57IKK227 pKa = 10.79LKK229 pKa = 10.65KK230 pKa = 10.22

Molecular weight:
26.68 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

715

0

715

274971

37

1971

384.6

43.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.768 ± 0.082

0.717 ± 0.023

5.307 ± 0.076

5.716 ± 0.127

5.105 ± 0.086

4.921 ± 0.106

1.959 ± 0.034

8.503 ± 0.098

9.221 ± 0.108

9.589 ± 0.096

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.773 ± 0.034

7.419 ± 0.088

3.452 ± 0.078

4.54 ± 0.092

3.331 ± 0.058

6.446 ± 0.064

5.557 ± 0.063

5.942 ± 0.074

0.985 ± 0.032

3.751 ± 0.057

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski