Circoviridae 11 LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; environmental samples

Average proteome isoelectric point is 8.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5TNE1|S5TNE1_9CIRC Uncharacterized protein OS=Circoviridae 11 LDMD-2013 OX=1379715 PE=4 SV=1
MM1 pKa = 6.89PTPRR5 pKa = 11.84GVYY8 pKa = 8.76WCWTLNNYY16 pKa = 7.76TPEE19 pKa = 4.19EE20 pKa = 4.01LSHH23 pKa = 6.9CRR25 pKa = 11.84TLCDD29 pKa = 3.04RR30 pKa = 11.84AEE32 pKa = 4.31DD33 pKa = 3.59VTYY36 pKa = 10.7VCFSRR41 pKa = 11.84EE42 pKa = 4.13VGDD45 pKa = 3.52SGTPHH50 pKa = 5.94LQGYY54 pKa = 9.69LEE56 pKa = 4.43LAAKK60 pKa = 10.08LRR62 pKa = 11.84LGGLRR67 pKa = 11.84KK68 pKa = 9.73LDD70 pKa = 3.64GLGRR74 pKa = 11.84AHH76 pKa = 7.52LEE78 pKa = 4.03LRR80 pKa = 11.84KK81 pKa = 8.78GTQAEE86 pKa = 4.33AIDD89 pKa = 3.97YY90 pKa = 9.52CRR92 pKa = 11.84KK93 pKa = 10.43DD94 pKa = 3.3GNEE97 pKa = 3.82TFEE100 pKa = 4.0EE101 pKa = 4.36HH102 pKa = 5.89GTRR105 pKa = 11.84KK106 pKa = 8.33TSSQGKK112 pKa = 9.89RR113 pKa = 11.84MDD115 pKa = 3.71LEE117 pKa = 4.2AVKK120 pKa = 10.8ALLDD124 pKa = 3.66GGAPLRR130 pKa = 11.84EE131 pKa = 4.2VADD134 pKa = 3.41QHH136 pKa = 6.6FGTFVRR142 pKa = 11.84YY143 pKa = 9.66GRR145 pKa = 11.84GIAQYY150 pKa = 11.18QLVTAKK156 pKa = 10.13KK157 pKa = 8.15RR158 pKa = 11.84ARR160 pKa = 11.84DD161 pKa = 3.67VSVHH165 pKa = 5.34VLWGAPGVGKK175 pKa = 8.12TRR177 pKa = 11.84WIFEE181 pKa = 3.98RR182 pKa = 11.84HH183 pKa = 6.56ADD185 pKa = 4.74DD186 pKa = 5.84LWICNDD192 pKa = 3.73PTLQWFDD199 pKa = 3.6GYY201 pKa = 10.9QGEE204 pKa = 4.69SVVLIDD210 pKa = 5.02DD211 pKa = 4.16FRR213 pKa = 11.84GEE215 pKa = 3.86ASPAFMLRR223 pKa = 11.84LLDD226 pKa = 4.49RR227 pKa = 11.84YY228 pKa = 9.47PLQVPIKK235 pKa = 10.58GGFTEE240 pKa = 4.41WRR242 pKa = 11.84ATTIYY247 pKa = 9.01ITSNVAPPFGMEE259 pKa = 4.66SIVAPLARR267 pKa = 11.84RR268 pKa = 11.84LSIVQEE274 pKa = 4.19LPKK277 pKa = 10.16QEE279 pKa = 3.99YY280 pKa = 10.34DD281 pKa = 3.06ITEE284 pKa = 4.01AALNEE289 pKa = 4.24IFNN292 pKa = 4.4

Molecular weight: 33.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5SYE3|S5SYE3_9CIRC Uncharacterized protein OS=Circoviridae 11 LDMD-2013 OX=1379715 PE=4 SV=1
MM1 pKa = 7.49RR2 pKa = 11.84VDD4 pKa = 3.56IPEE7 pKa = 3.83MGYY10 pKa = 10.35NLVSRR15 pKa = 11.84DD16 pKa = 2.93VDD18 pKa = 5.29LIILLYY24 pKa = 10.58ARR26 pKa = 11.84IWRR29 pKa = 11.84RR30 pKa = 11.84NMPALANTRR39 pKa = 11.84IDD41 pKa = 3.38VAGTINALYY50 pKa = 10.23HH51 pKa = 5.24STNISRR57 pKa = 11.84GPDD60 pKa = 3.06SRR62 pKa = 11.84RR63 pKa = 11.84NGPLNSVLSTLNSKK77 pKa = 10.02IPAFFLIRR85 pKa = 11.84TSTFHH90 pKa = 7.08LAADD94 pKa = 3.73HH95 pKa = 6.39FLHH98 pKa = 7.35FEE100 pKa = 4.17GQTATTATATPSPFASILFTISHH123 pKa = 7.07

Molecular weight: 13.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

837

100

292

167.4

19.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.766 ± 0.879

1.553 ± 0.619

5.018 ± 0.609

5.496 ± 1.21

3.226 ± 0.557

6.571 ± 1.045

2.987 ± 0.455

6.332 ± 1.126

6.93 ± 2.48

8.005 ± 1.636

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.389 ± 0.554

3.943 ± 0.674

3.704 ± 0.679

3.226 ± 0.575

8.363 ± 0.582

6.452 ± 1.414

6.332 ± 0.691

5.018 ± 0.584

1.912 ± 0.507

4.421 ± 0.547
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski