Roseomonas sp. CQN31

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Roseomonas

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5423 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A317FD27|A0A317FD27_9PROT Uncharacterized protein OS=Roseomonas sp. CQN31 OX=2184016 GN=DFH01_20465 PE=3 SV=1
MM1 pKa = 7.16ATVSINFGDD10 pKa = 4.75DD11 pKa = 2.93NDD13 pKa = 3.87NFYY16 pKa = 10.65GQPYY20 pKa = 10.42DD21 pKa = 4.42DD22 pKa = 5.63DD23 pKa = 6.4LIIQMGKK30 pKa = 10.87GNDD33 pKa = 3.27TVFAGEE39 pKa = 4.25GTNLVYY45 pKa = 10.75LGAGNDD51 pKa = 3.57VFEE54 pKa = 5.51SEE56 pKa = 5.16DD57 pKa = 3.49MLGGPFDD64 pKa = 4.29EE65 pKa = 4.9STEE68 pKa = 4.35CGPSTVYY75 pKa = 11.22GDD77 pKa = 3.44IGNDD81 pKa = 2.99LFDD84 pKa = 5.37FYY86 pKa = 11.36DD87 pKa = 3.66VSGTAYY93 pKa = 10.18GGKK96 pKa = 10.34DD97 pKa = 2.94NDD99 pKa = 3.34IYY101 pKa = 10.88LIKK104 pKa = 10.91GMDD107 pKa = 3.25ASVSVVEE114 pKa = 4.43YY115 pKa = 10.23GGEE118 pKa = 4.32GIDD121 pKa = 4.5TIKK124 pKa = 10.45IDD126 pKa = 3.73LWSKK130 pKa = 9.02ATYY133 pKa = 7.96TIPVNVEE140 pKa = 3.6NLSVGNVYY148 pKa = 10.65YY149 pKa = 10.2WGFFSNTWEE158 pKa = 4.07TTNNSFEE165 pKa = 4.33EE166 pKa = 4.94GFVDD170 pKa = 4.83AGVTIIGNAEE180 pKa = 3.69NNAITGSDD188 pKa = 3.1MHH190 pKa = 6.77DD191 pKa = 3.58TLKK194 pKa = 11.13GGAGADD200 pKa = 3.68TVKK203 pKa = 10.91GGLANDD209 pKa = 3.7VLHH212 pKa = 7.03GEE214 pKa = 4.78DD215 pKa = 4.56GNDD218 pKa = 3.69VLWGEE223 pKa = 4.4HH224 pKa = 6.42GNDD227 pKa = 3.41KK228 pKa = 11.01AYY230 pKa = 10.76GGIGNDD236 pKa = 3.42KK237 pKa = 10.97LSGHH241 pKa = 6.72GGNDD245 pKa = 3.31TLDD248 pKa = 3.59GGEE251 pKa = 4.49NDD253 pKa = 4.68DD254 pKa = 5.23LVDD257 pKa = 4.01GGGGNDD263 pKa = 4.26LLYY266 pKa = 10.99GGNGKK271 pKa = 9.64DD272 pKa = 3.56VLFDD276 pKa = 3.47GAGADD281 pKa = 3.86TMNGGAGDD289 pKa = 4.92DD290 pKa = 3.72IYY292 pKa = 11.75VVTDD296 pKa = 3.3ATDD299 pKa = 3.5KK300 pKa = 11.07VIEE303 pKa = 4.21AAGGGNDD310 pKa = 4.08LVKK313 pKa = 10.19TKK315 pKa = 10.38LASYY319 pKa = 8.93TLGSEE324 pKa = 4.43VEE326 pKa = 4.34SLMFTGSGGFQGTGNALTNRR346 pKa = 11.84ITGGSGADD354 pKa = 3.74LLKK357 pKa = 11.29GMDD360 pKa = 4.09GNDD363 pKa = 3.07VLTGNDD369 pKa = 4.23GADD372 pKa = 3.26QLYY375 pKa = 9.48TGTGNDD381 pKa = 3.61YY382 pKa = 11.37ASGGNGIDD390 pKa = 3.61SLFGEE395 pKa = 4.96AGNDD399 pKa = 3.23SLYY402 pKa = 11.18GNNDD406 pKa = 2.97ADD408 pKa = 4.12YY409 pKa = 9.24LTGGGDD415 pKa = 3.61NDD417 pKa = 4.18YY418 pKa = 11.47LDD420 pKa = 4.89GGAGNDD426 pKa = 3.95NMHH429 pKa = 7.08GDD431 pKa = 4.24DD432 pKa = 5.12GHH434 pKa = 7.04DD435 pKa = 3.35TMQGGDD441 pKa = 4.36GNDD444 pKa = 3.48LMQGGAGNDD453 pKa = 3.76EE454 pKa = 3.91IRR456 pKa = 11.84GGNGDD461 pKa = 3.75DD462 pKa = 4.58KK463 pKa = 11.24IYY465 pKa = 11.22GGLGKK470 pKa = 10.34DD471 pKa = 3.71KK472 pKa = 10.93LYY474 pKa = 11.34GDD476 pKa = 4.53AGFDD480 pKa = 3.47SFSYY484 pKa = 10.72QSLNEE489 pKa = 3.97SNAAFGRR496 pKa = 11.84DD497 pKa = 3.98FIYY500 pKa = 10.42GFEE503 pKa = 3.97QGKK506 pKa = 9.96DD507 pKa = 3.38RR508 pKa = 11.84IDD510 pKa = 4.08LSWIDD515 pKa = 3.79ADD517 pKa = 3.86STVAGNQGFNFTGSGAFFKK536 pKa = 10.98SAGDD540 pKa = 3.41LWLTSSLLGATVNCDD555 pKa = 3.24VNGDD559 pKa = 3.82AVADD563 pKa = 3.74MQINVVGVWNLTASDD578 pKa = 4.18FVLL581 pKa = 4.61

Molecular weight: 60.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A317FHE3|A0A317FHE3_9PROT HTH marR-type domain-containing protein OS=Roseomonas sp. CQN31 OX=2184016 GN=DFH01_02640 PE=4 SV=1
MM1 pKa = 7.56PRR3 pKa = 11.84ACSAARR9 pKa = 11.84TSWKK13 pKa = 9.88PSPPPSRR20 pKa = 11.84RR21 pKa = 11.84SARR24 pKa = 11.84RR25 pKa = 11.84PPSRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84PSPPRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84WWPRR45 pKa = 11.84SRR47 pKa = 11.84KK48 pKa = 8.97SPRR51 pKa = 11.84AARR54 pKa = 11.84TPSSHH59 pKa = 3.92WRR61 pKa = 11.84KK62 pKa = 9.39RR63 pKa = 11.84ATPPPTRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84APRR75 pKa = 11.84WMARR79 pKa = 11.84RR80 pKa = 11.84SSCARR85 pKa = 11.84SASSGRR91 pKa = 11.84WRR93 pKa = 3.76

Molecular weight: 11.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5423

0

5423

1823058

26

9467

336.2

35.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.265 ± 0.068

0.817 ± 0.011

5.213 ± 0.055

5.431 ± 0.034

3.361 ± 0.021

9.676 ± 0.06

1.929 ± 0.02

4.026 ± 0.027

1.841 ± 0.026

10.823 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.297 ± 0.021

2.052 ± 0.024

6.162 ± 0.04

2.845 ± 0.019

8.348 ± 0.051

4.251 ± 0.027

4.99 ± 0.043

7.499 ± 0.029

1.507 ± 0.015

1.667 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski