Lactococcus phage 4268

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8LTK9|Q8LTK9_9CAUD Major structural protein OS=Lactococcus phage 4268 OX=201847 GN=orf35 PE=4 SV=1
MM1 pKa = 7.36SVTVDD6 pKa = 4.12DD7 pKa = 6.04LLDD10 pKa = 3.62QLSEE14 pKa = 4.54DD15 pKa = 5.09DD16 pKa = 4.43DD17 pKa = 4.84RR18 pKa = 11.84KK19 pKa = 10.14PQLQIYY25 pKa = 9.45FDD27 pKa = 3.58TATAYY32 pKa = 10.31VKK34 pKa = 10.74NAVSSDD40 pKa = 3.45TVDD43 pKa = 3.35APFFNVEE50 pKa = 4.24NVSPIYY56 pKa = 10.5DD57 pKa = 3.71VAVLSYY63 pKa = 11.85SMDD66 pKa = 2.86LWINRR71 pKa = 11.84STTMPPTTAVDD82 pKa = 3.39HH83 pKa = 6.19MVGQLRR89 pKa = 11.84GLYY92 pKa = 10.16SSWKK96 pKa = 9.74EE97 pKa = 3.82EE98 pKa = 3.68QGGQNLQTEE107 pKa = 4.62

Molecular weight: 12.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8LTL5|Q8LTL5_9CAUD Uncharacterized protein orf28 OS=Lactococcus phage 4268 OX=201847 GN=orf28 PE=4 SV=1
MM1 pKa = 7.78PMTGRR6 pKa = 11.84CRR8 pKa = 11.84EE9 pKa = 4.3PNCHH13 pKa = 5.93AVVIRR18 pKa = 11.84PLHH21 pKa = 5.91YY22 pKa = 9.15CTKK25 pKa = 10.35HH26 pKa = 5.78ADD28 pKa = 3.25KK29 pKa = 10.53EE30 pKa = 4.12AAYY33 pKa = 9.83QASRR37 pKa = 11.84EE38 pKa = 3.85RR39 pKa = 11.84WTNRR43 pKa = 11.84NDD45 pKa = 3.1NTKK48 pKa = 10.29RR49 pKa = 11.84YY50 pKa = 9.92KK51 pKa = 10.61DD52 pKa = 3.39YY53 pKa = 11.28NKK55 pKa = 10.4RR56 pKa = 11.84KK57 pKa = 9.81RR58 pKa = 11.84EE59 pKa = 3.76YY60 pKa = 10.79SDD62 pKa = 3.68IKK64 pKa = 10.58VEE66 pKa = 4.11QNKK69 pKa = 9.42FYY71 pKa = 10.67QSKK74 pKa = 7.19QWKK77 pKa = 9.1SIRR80 pKa = 11.84DD81 pKa = 3.5VVRR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84DD87 pKa = 3.45NFLCQYY93 pKa = 9.72CKK95 pKa = 10.03AHH97 pKa = 5.68NRR99 pKa = 11.84VRR101 pKa = 11.84TGKK104 pKa = 9.76IVDD107 pKa = 4.34HH108 pKa = 6.43IVPVEE113 pKa = 3.65FDD115 pKa = 4.11LNGKK119 pKa = 6.92TVMDD123 pKa = 4.61NLAFCCSKK131 pKa = 10.58CHH133 pKa = 5.55TRR135 pKa = 11.84KK136 pKa = 8.24TKK138 pKa = 9.8WEE140 pKa = 3.82QIYY143 pKa = 11.02YY144 pKa = 8.08GTGYY148 pKa = 10.91GNKK151 pKa = 8.24TKK153 pKa = 10.92NVIPIKK159 pKa = 10.2NVKK162 pKa = 9.75DD163 pKa = 3.58VPDD166 pKa = 3.84FQKK169 pKa = 11.1NEE171 pKa = 3.83RR172 pKa = 3.91

Molecular weight: 20.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11348

54

1711

231.6

25.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.023 ± 0.72

0.529 ± 0.18

6.45 ± 0.275

6.345 ± 0.516

4.001 ± 0.319

6.935 ± 0.579

1.251 ± 0.183

7.006 ± 0.315

8.407 ± 0.597

7.481 ± 0.43

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.749 ± 0.252

6.309 ± 0.299

2.644 ± 0.275

4.6 ± 0.279

3.313 ± 0.367

7.385 ± 0.427

6.706 ± 0.798

5.754 ± 0.248

1.463 ± 0.211

3.648 ± 0.319
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski