Rhodococcus sp. WMMA185

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; unclassified Rhodococcus

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3688 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8T8N5|A0A1D8T8N5_9NOCA Uncharacterized protein OS=Rhodococcus sp. WMMA185 OX=679318 GN=BFN03_17305 PE=4 SV=1
MM1 pKa = 8.0AITPNGTRR9 pKa = 11.84AYY11 pKa = 8.37VTNIGDD17 pKa = 3.69NTVSVIDD24 pKa = 3.73TATNTVIGAPIPVGGAPIGVAITPNGTRR52 pKa = 11.84AYY54 pKa = 8.7VTNNGDD60 pKa = 3.4NTVSVIDD67 pKa = 3.73TATNTVIGAPIPVGATPQGVAITPNGTRR95 pKa = 11.84AYY97 pKa = 8.51VTNAGDD103 pKa = 3.6NTVSVIDD110 pKa = 3.9TATDD114 pKa = 3.91TIVDD118 pKa = 3.45ATIAVGNFPNAVAITPDD135 pKa = 3.06GTRR138 pKa = 11.84AYY140 pKa = 8.65VTNRR144 pKa = 11.84FNDD147 pKa = 3.95TVSVIAIDD155 pKa = 3.37QCLGSLCIDD164 pKa = 4.4FGSLTGSGSGAGSTFSS180 pKa = 3.51

Molecular weight: 17.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8T6H7|A0A1D8T6H7_9NOCA Uncharacterized protein OS=Rhodococcus sp. WMMA185 OX=679318 GN=BFN03_12795 PE=4 SV=1
MM1 pKa = 6.94VSVVAVTVRR10 pKa = 11.84GVKK13 pKa = 9.73QVVRR17 pKa = 11.84VKK19 pKa = 10.75LLADD23 pKa = 3.79SQQLDD28 pKa = 3.61AVVRR32 pKa = 11.84TLAVCNDD39 pKa = 3.32TATLVARR46 pKa = 11.84IAHH49 pKa = 5.56EE50 pKa = 3.43RR51 pKa = 11.84RR52 pKa = 11.84VFRR55 pKa = 11.84KK56 pKa = 9.37RR57 pKa = 11.84DD58 pKa = 3.39LRR60 pKa = 11.84AITYY64 pKa = 7.57ATARR68 pKa = 11.84EE69 pKa = 4.08HH70 pKa = 6.77GGLGAQVAQSVIRR83 pKa = 11.84KK84 pKa = 8.87VADD87 pKa = 3.21SYY89 pKa = 11.56TSLRR93 pKa = 11.84ANLRR97 pKa = 11.84NGRR100 pKa = 11.84YY101 pKa = 9.73GPTGSRR107 pKa = 11.84RR108 pKa = 11.84RR109 pKa = 11.84TRR111 pKa = 11.84VEE113 pKa = 3.33NTPIRR118 pKa = 11.84FRR120 pKa = 11.84PDD122 pKa = 2.62AAQPFDD128 pKa = 4.58DD129 pKa = 5.37RR130 pKa = 11.84CLSWGYY136 pKa = 10.91DD137 pKa = 3.54PNDD140 pKa = 3.57TGGGSVSIWTVDD152 pKa = 3.14GRR154 pKa = 11.84VKK156 pKa = 10.59NLRR159 pKa = 11.84FVGHH163 pKa = 7.36PDD165 pKa = 2.97QVAMLRR171 pKa = 11.84QYY173 pKa = 11.25RR174 pKa = 11.84KK175 pKa = 10.48GEE177 pKa = 3.87TDD179 pKa = 3.55LVVQRR184 pKa = 11.84DD185 pKa = 3.6RR186 pKa = 11.84RR187 pKa = 11.84GTPTAYY193 pKa = 10.82LIATCDD199 pKa = 3.59VPDD202 pKa = 4.48PPVASGDD209 pKa = 3.82HH210 pKa = 6.81LNTPDD215 pKa = 2.83GWIGVDD221 pKa = 3.47LGIVNMAVASDD232 pKa = 4.15RR233 pKa = 11.84EE234 pKa = 4.44LGDD237 pKa = 3.78HH238 pKa = 7.48LIDD241 pKa = 3.81TFGDD245 pKa = 4.17GAPDD249 pKa = 3.97GPAGRR254 pKa = 11.84GAVKK258 pKa = 10.36DD259 pKa = 3.21RR260 pKa = 11.84RR261 pKa = 11.84TRR263 pKa = 11.84NRR265 pKa = 11.84EE266 pKa = 3.66LRR268 pKa = 11.84HH269 pKa = 4.46TLQRR273 pKa = 11.84KK274 pKa = 4.74NTKK277 pKa = 7.99SAKK280 pKa = 8.89RR281 pKa = 11.84LLRR284 pKa = 11.84RR285 pKa = 11.84RR286 pKa = 11.84ARR288 pKa = 11.84KK289 pKa = 8.04EE290 pKa = 3.41ARR292 pKa = 11.84FAADD296 pKa = 3.1VNHH299 pKa = 6.34QISKK303 pKa = 10.35KK304 pKa = 8.64VVAVAEE310 pKa = 4.13RR311 pKa = 11.84TGRR314 pKa = 11.84GIALEE319 pKa = 4.4DD320 pKa = 3.17LTGIRR325 pKa = 11.84EE326 pKa = 3.94RR327 pKa = 11.84VRR329 pKa = 11.84FRR331 pKa = 11.84KK332 pKa = 8.65PQRR335 pKa = 11.84ATHH338 pKa = 5.88SSWAFAQLGAFISYY352 pKa = 9.24KK353 pKa = 9.43AARR356 pKa = 11.84VGVPVMSVDD365 pKa = 3.47PANTSRR371 pKa = 11.84RR372 pKa = 11.84CIRR375 pKa = 11.84CGHH378 pKa = 6.45IDD380 pKa = 3.56KK381 pKa = 10.72KK382 pKa = 10.9NRR384 pKa = 11.84PHH386 pKa = 7.32QDD388 pKa = 2.71RR389 pKa = 11.84FCCLDD394 pKa = 3.46CGFVEE399 pKa = 4.55HH400 pKa = 7.12ADD402 pKa = 3.06IVGSHH407 pKa = 6.79NIAHH411 pKa = 7.38RR412 pKa = 11.84AAEE415 pKa = 4.38DD416 pKa = 3.36YY417 pKa = 10.14RR418 pKa = 11.84AQSTVPGAAA427 pKa = 3.42

Molecular weight: 47.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3688

0

3688

1232631

31

8938

334.2

35.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.161 ± 0.046

0.758 ± 0.012

6.32 ± 0.035

5.779 ± 0.034

3.092 ± 0.024

9.052 ± 0.039

2.081 ± 0.016

4.447 ± 0.03

2.2 ± 0.033

9.791 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.963 ± 0.015

2.257 ± 0.021

5.491 ± 0.035

2.792 ± 0.021

7.001 ± 0.039

6.169 ± 0.026

6.171 ± 0.034

9.033 ± 0.046

1.386 ± 0.016

2.059 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski