Mycobacterium phage Jsquared

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1N1Z8|A0A2P1N1Z8_9CAUD Uncharacterized protein OS=Mycobacterium phage Jsquared OX=2126814 GN=95 PE=4 SV=1
MM1 pKa = 7.76IEE3 pKa = 4.01TLGDD7 pKa = 3.43IDD9 pKa = 4.7QMARR13 pKa = 11.84SPKK16 pKa = 9.18TGRR19 pKa = 11.84EE20 pKa = 4.06PGDD23 pKa = 3.18NTYY26 pKa = 10.92NCTWAAVALSAYY38 pKa = 7.91TKK40 pKa = 10.87VVGGDD45 pKa = 3.57DD46 pKa = 4.11NEE48 pKa = 4.47VGTQISDD55 pKa = 3.68LLGDD59 pKa = 4.65LLHH62 pKa = 6.89LADD65 pKa = 3.94AAGVDD70 pKa = 4.61FYY72 pKa = 11.56EE73 pKa = 4.65ALATAEE79 pKa = 4.27TNYY82 pKa = 10.12KK83 pKa = 10.72AEE85 pKa = 4.43VNGEE89 pKa = 3.97

Molecular weight: 9.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1N1Z4|A0A2P1N1Z4_9CAUD Uncharacterized protein OS=Mycobacterium phage Jsquared OX=2126814 GN=82 PE=4 SV=1
MM1 pKa = 7.9PPRR4 pKa = 11.84ASIQQTADD12 pKa = 3.33YY13 pKa = 10.78LGVHH17 pKa = 7.09PNTVRR22 pKa = 11.84NYY24 pKa = 10.05IADD27 pKa = 4.05GKK29 pKa = 10.59LKK31 pKa = 10.44AVRR34 pKa = 11.84LGPRR38 pKa = 11.84LIRR41 pKa = 11.84VEE43 pKa = 4.05RR44 pKa = 11.84DD45 pKa = 3.15SVEE48 pKa = 3.78ALMRR52 pKa = 11.84PIGKK56 pKa = 9.51

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

101

0

101

16486

25

837

163.2

18.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.657 ± 0.29

0.91 ± 0.103

6.339 ± 0.242

7.091 ± 0.361

3.445 ± 0.171

8.225 ± 0.454

2.081 ± 0.163

4.913 ± 0.182

4.913 ± 0.277

8.08 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.566 ± 0.142

3.3 ± 0.159

5.271 ± 0.249

3.627 ± 0.225

6.606 ± 0.377

5.077 ± 0.192

5.829 ± 0.29

7.121 ± 0.205

1.959 ± 0.13

2.99 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski