Dioscorea bacilliform RT virus 2

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Caulimoviridae; Badnavirus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G1E451|A0A3G1E451_9VIRU ORF1 protein OS=Dioscorea bacilliform RT virus 2 OX=2011125 PE=4 SV=1
MM1 pKa = 7.33NSEE4 pKa = 3.84SLEE7 pKa = 4.12YY8 pKa = 10.39KK9 pKa = 10.27RR10 pKa = 11.84ALQAVEE16 pKa = 4.43SLEE19 pKa = 4.19PPALGFIKK27 pKa = 10.41PADD30 pKa = 3.88FRR32 pKa = 11.84AGLATASAAVQKK44 pKa = 10.64QNNTIIQLLLQLSEE58 pKa = 4.7KK59 pKa = 10.6VNSLQAEE66 pKa = 3.89IRR68 pKa = 11.84TLRR71 pKa = 11.84KK72 pKa = 9.79EE73 pKa = 4.0PEE75 pKa = 4.35LPDD78 pKa = 4.69EE79 pKa = 4.79ILDD82 pKa = 3.94QITSRR87 pKa = 11.84FNKK90 pKa = 10.14LSIAEE95 pKa = 4.38APSEE99 pKa = 3.82PRR101 pKa = 11.84GRR103 pKa = 11.84ILVHH107 pKa = 6.07TDD109 pKa = 2.94PVKK112 pKa = 10.55IFEE115 pKa = 4.15EE116 pKa = 4.47EE117 pKa = 4.05KK118 pKa = 11.08LKK120 pKa = 11.17LL121 pKa = 3.66

Molecular weight: 13.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G1E455|A0A3G1E455_9VIRU Reverse transcriptase OS=Dioscorea bacilliform RT virus 2 OX=2011125 PE=4 SV=1
MM1 pKa = 6.89SVKK4 pKa = 9.55WEE6 pKa = 4.28KK7 pKa = 10.66SVQAYY12 pKa = 9.75FEE14 pKa = 4.28SHH16 pKa = 6.23KK17 pKa = 10.98PEE19 pKa = 4.84LEE21 pKa = 3.93YY22 pKa = 11.24LDD24 pKa = 5.29LSSLEE29 pKa = 4.12KK30 pKa = 10.61PPLKK34 pKa = 10.71SIAHH38 pKa = 5.79NLAYY42 pKa = 10.72LNYY45 pKa = 9.56QEE47 pKa = 5.24KK48 pKa = 10.16LQSKK52 pKa = 9.82VLLKK56 pKa = 10.44HH57 pKa = 6.7LYY59 pKa = 9.13TIQEE63 pKa = 4.47KK64 pKa = 10.31ISEE67 pKa = 4.3LEE69 pKa = 4.03AEE71 pKa = 4.57NKK73 pKa = 9.81RR74 pKa = 11.84LRR76 pKa = 11.84KK77 pKa = 10.01DD78 pKa = 3.4LVSLTKK84 pKa = 10.34EE85 pKa = 4.17VVEE88 pKa = 4.1NRR90 pKa = 11.84PLTEE94 pKa = 3.92RR95 pKa = 11.84KK96 pKa = 9.28VQEE99 pKa = 4.54LIVNITSQPKK109 pKa = 9.97EE110 pKa = 3.85IEE112 pKa = 4.01QQAVKK117 pKa = 10.47LSKK120 pKa = 10.54DD121 pKa = 3.4LQLKK125 pKa = 9.69LDD127 pKa = 3.69RR128 pKa = 11.84VEE130 pKa = 5.59DD131 pKa = 3.28ILRR134 pKa = 11.84RR135 pKa = 11.84LEE137 pKa = 4.13GAVTVV142 pKa = 4.14

Molecular weight: 16.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2156

121

1893

718.7

81.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.772 ± 0.856

1.577 ± 0.71

4.314 ± 0.414

9.369 ± 1.209

3.711 ± 0.763

5.798 ± 1.845

2.319 ± 0.464

6.401 ± 0.346

7.375 ± 1.083

9.416 ± 2.508

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.505 ± 0.778

3.896 ± 0.085

4.314 ± 0.62

4.777 ± 0.563

5.288 ± 0.133

6.772 ± 0.361

5.659 ± 0.807

5.705 ± 0.605

0.974 ± 0.32

3.061 ± 0.62
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski