Escherichia phage Minorna

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Drulisvirus; Escherichia virus Minorna

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482IFG4|A0A482IFG4_9CAUD Uncharacterized protein OS=Escherichia phage Minorna OX=2547246 GN=CPT_Minorna_021 PE=4 SV=1
MM1 pKa = 7.66AYY3 pKa = 9.82SWQEE7 pKa = 3.71QIKK10 pKa = 9.53PAGTQDD16 pKa = 3.22IQCDD20 pKa = 3.23IEE22 pKa = 4.59YY23 pKa = 10.49LDD25 pKa = 3.51KK26 pKa = 11.27SYY28 pKa = 10.95IHH30 pKa = 6.76VYY32 pKa = 10.56LDD34 pKa = 3.57GVEE37 pKa = 4.31TTGYY41 pKa = 8.08TWTSSTNIRR50 pKa = 11.84LNTALTADD58 pKa = 3.76TTVLLIRR65 pKa = 11.84KK66 pKa = 6.77TEE68 pKa = 3.78RR69 pKa = 11.84EE70 pKa = 3.71YY71 pKa = 11.31LYY73 pKa = 10.39IEE75 pKa = 4.49FASGSPFIEE84 pKa = 4.25VNVDD88 pKa = 3.39SQNTQFLHH96 pKa = 6.59LAQEE100 pKa = 4.35LVEE103 pKa = 4.47GRR105 pKa = 11.84AIPGFYY111 pKa = 10.72GDD113 pKa = 3.83ISMNGYY119 pKa = 10.46RR120 pKa = 11.84ITNLGDD126 pKa = 3.46GVNPGDD132 pKa = 4.55AVNKK136 pKa = 9.6GQLDD140 pKa = 3.86VVDD143 pKa = 4.48ARR145 pKa = 11.84VTALEE150 pKa = 3.92QGINTDD156 pKa = 3.65DD157 pKa = 4.12VTIPWCTVTTVPTNIISPPYY177 pKa = 9.07RR178 pKa = 11.84FTRR181 pKa = 11.84ATLYY185 pKa = 10.92LDD187 pKa = 5.29GVHH190 pKa = 6.35QDD192 pKa = 2.48IGYY195 pKa = 9.91GFSIVNNQIILAEE208 pKa = 4.43TIPAGTVVSVRR219 pKa = 11.84IGQDD223 pKa = 3.03STDD226 pKa = 3.56LDD228 pKa = 3.61AVSRR232 pKa = 11.84SEE234 pKa = 4.49FDD236 pKa = 3.42DD237 pKa = 4.0LKK239 pKa = 10.92EE240 pKa = 3.88YY241 pKa = 10.1VYY243 pKa = 11.65NMDD246 pKa = 4.59LGEE249 pKa = 4.06LL250 pKa = 3.82

Molecular weight: 27.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482IIX1|A0A482IIX1_9CAUD Terminase small subunit OS=Escherichia phage Minorna OX=2547246 GN=CPT_Minorna_008 PE=4 SV=1
MM1 pKa = 7.1YY2 pKa = 10.08GKK4 pKa = 9.94NPEE7 pKa = 3.98VLLMRR12 pKa = 11.84KK13 pKa = 7.4QQPTIDD19 pKa = 3.64GLARR23 pKa = 11.84EE24 pKa = 4.46YY25 pKa = 9.54TAKK28 pKa = 10.66AALRR32 pKa = 11.84QHH34 pKa = 6.15YY35 pKa = 7.59EE36 pKa = 3.85KK37 pKa = 10.33QAKK40 pKa = 8.74RR41 pKa = 11.84LGMTLRR47 pKa = 11.84GYY49 pKa = 8.15CQRR52 pKa = 11.84FNVRR56 pKa = 11.84GVII59 pKa = 3.65

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

13665

38

1232

239.7

26.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.355 ± 0.502

1.259 ± 0.211

6.301 ± 0.262

5.62 ± 0.298

3.015 ± 0.139

7.501 ± 0.242

1.939 ± 0.145

4.705 ± 0.183

4.903 ± 0.274

8.957 ± 0.237

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.839 ± 0.18

4.105 ± 0.283

3.835 ± 0.237

4.654 ± 0.365

5.664 ± 0.282

6.22 ± 0.284

5.796 ± 0.278

6.981 ± 0.206

1.464 ± 0.111

3.886 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski