Drosophila melanogaster sigma virus (isolate Drosophila/USA/AP30/2005) (DMelSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sigmavirus; Drosophila melanogaster sigmavirus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A7WNB3|GLYCO_DMSVA Glycoprotein OS=Drosophila melanogaster sigma virus (isolate Drosophila/USA/AP30/2005) OX=666363 GN=G PE=3 SV=1
MM1 pKa = 7.54NLTQEE6 pKa = 4.33DD7 pKa = 4.31EE8 pKa = 4.08QRR10 pKa = 11.84LALLRR15 pKa = 11.84EE16 pKa = 4.12RR17 pKa = 11.84AAAWDD22 pKa = 3.63HH23 pKa = 6.46KK24 pKa = 10.8NALGEE29 pKa = 4.23FEE31 pKa = 5.81SNAEE35 pKa = 3.64LDD37 pKa = 3.88VYY39 pKa = 11.37NEE41 pKa = 4.62GIAYY45 pKa = 7.87PPSIDD50 pKa = 3.28EE51 pKa = 4.33MEE53 pKa = 4.33VGEE56 pKa = 5.36GRR58 pKa = 11.84PTLDD62 pKa = 4.73KK63 pKa = 10.76ILEE66 pKa = 4.1SSIGSDD72 pKa = 3.35SLPNLEE78 pKa = 5.66DD79 pKa = 4.12GPPDD83 pKa = 3.29QEE85 pKa = 4.05AAYY88 pKa = 10.18HH89 pKa = 5.52EE90 pKa = 4.71GEE92 pKa = 4.52MYY94 pKa = 9.47STMMTSYY101 pKa = 10.32MDD103 pKa = 3.51QDD105 pKa = 4.17NEE107 pKa = 4.53NMDD110 pKa = 4.49LSAGPTCSTPQPITGIHH127 pKa = 6.11HH128 pKa = 6.96KK129 pKa = 8.77EE130 pKa = 4.18TINGRR135 pKa = 11.84VTQMVYY141 pKa = 10.01IGEE144 pKa = 4.29TSDD147 pKa = 3.03MALINRR153 pKa = 11.84LQDD156 pKa = 3.57EE157 pKa = 4.43FDD159 pKa = 3.56VLYY162 pKa = 9.15NTYY165 pKa = 10.6PNRR168 pKa = 11.84NKK170 pKa = 10.18QGEE173 pKa = 4.4IVHH176 pKa = 6.68LIVMWSTPLKK186 pKa = 10.1PSTRR190 pKa = 11.84YY191 pKa = 9.89SLSPAQPPGKK201 pKa = 9.49KK202 pKa = 9.58RR203 pKa = 11.84KK204 pKa = 9.05IVQHH208 pKa = 5.96SSNPEE213 pKa = 3.65PEE215 pKa = 4.06IVFVPEE221 pKa = 4.2PTSSEE226 pKa = 3.6QSLPEE231 pKa = 4.01RR232 pKa = 11.84QIKK235 pKa = 10.25AYY237 pKa = 8.41LQRR240 pKa = 11.84GIEE243 pKa = 4.16LKK245 pKa = 10.67GKK247 pKa = 9.54SAEE250 pKa = 4.11VPNFKK255 pKa = 8.75ITNEE259 pKa = 4.01TLGFSDD265 pKa = 5.4ANLKK269 pKa = 10.15LLYY272 pKa = 9.26PDD274 pKa = 3.44TSLYY278 pKa = 9.8PDD280 pKa = 3.49NPVFILEE287 pKa = 4.57DD288 pKa = 3.48VFASTRR294 pKa = 11.84QLNKK298 pKa = 10.15IKK300 pKa = 10.79VNYY303 pKa = 9.29QLYY306 pKa = 10.64DD307 pKa = 3.47PVLPSII313 pKa = 4.68

Molecular weight: 35.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A7WNB2|PHOSP_DMSVA Phosphoprotein OS=Drosophila melanogaster sigma virus (isolate Drosophila/USA/AP30/2005) OX=666363 GN=P PE=3 SV=2
MM1 pKa = 7.43NKK3 pKa = 9.12MNQLVRR9 pKa = 11.84FVKK12 pKa = 10.03DD13 pKa = 3.36TVAVRR18 pKa = 11.84KK19 pKa = 8.24PQSEE23 pKa = 4.34DD24 pKa = 2.85KK25 pKa = 10.77SYY27 pKa = 11.55LPIPSTIGGHH37 pKa = 5.16EE38 pKa = 4.31VNSPFAEE45 pKa = 4.11PTAPSLGIIQPKK57 pKa = 9.46CKK59 pKa = 10.12RR60 pKa = 11.84ADD62 pKa = 3.41WLIKK66 pKa = 10.12SHH68 pKa = 6.41LTITTNYY75 pKa = 9.13EE76 pKa = 3.56IKK78 pKa = 10.27EE79 pKa = 4.06WEE81 pKa = 4.01TWDD84 pKa = 4.09RR85 pKa = 11.84AISDD89 pKa = 3.89ILDD92 pKa = 4.21LYY94 pKa = 11.07DD95 pKa = 4.0GNPVFKK101 pKa = 10.42PILLFVYY108 pKa = 10.2YY109 pKa = 10.35VLAYY113 pKa = 9.41NARR116 pKa = 11.84KK117 pKa = 9.66IPGPSNGVRR126 pKa = 11.84YY127 pKa = 8.85GAYY130 pKa = 9.82FDD132 pKa = 4.64EE133 pKa = 5.29LTTVWHH139 pKa = 7.39AIPEE143 pKa = 4.24LMNQEE148 pKa = 3.08IDD150 pKa = 3.12YY151 pKa = 10.44SYY153 pKa = 10.98NHH155 pKa = 6.79RR156 pKa = 11.84VLHH159 pKa = 6.44RR160 pKa = 11.84KK161 pKa = 7.27IQYY164 pKa = 9.5VISFKK169 pKa = 10.67IQMSSTKK176 pKa = 10.13RR177 pKa = 11.84RR178 pKa = 11.84TSPIEE183 pKa = 3.66SFIEE187 pKa = 4.23VTSEE191 pKa = 4.07GLKK194 pKa = 8.66HH195 pKa = 5.87TPQFTTILDD204 pKa = 3.58RR205 pKa = 11.84ARR207 pKa = 11.84FVYY210 pKa = 10.56SLTGGRR216 pKa = 11.84YY217 pKa = 9.15VIHH220 pKa = 7.08PFF222 pKa = 3.4

Molecular weight: 25.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3973

222

2129

662.2

75.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.531 ± 0.331

1.435 ± 0.246

5.462 ± 0.337

5.613 ± 0.57

4.027 ± 0.358

5.965 ± 0.446

2.794 ± 0.25

6.746 ± 0.3

4.757 ± 0.374

9.791 ± 0.584

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.794 ± 0.129

4.656 ± 0.228

5.412 ± 0.482

3.624 ± 0.142

5.437 ± 0.355

8.205 ± 0.456

6.771 ± 0.513

6.443 ± 0.481

1.611 ± 0.34

3.927 ± 0.295
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski