Pteropine orthoreovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Orthoreovirus; unclassified Orthoreovirus

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A1CEU6|A0A0A1CEU6_9REOV SNS (Fragment) OS=Pteropine orthoreovirus OX=1459003 PE=4 SV=1
MM1 pKa = 7.32MGNATSIVQNFNIKK15 pKa = 10.61GDD17 pKa = 3.73GNTFSPSAEE26 pKa = 4.24TASSAVPSLSLNPGLLNPGGKK47 pKa = 9.45AWTLIDD53 pKa = 3.53PTVPATAPGSLRR65 pKa = 11.84LMTTEE70 pKa = 4.89DD71 pKa = 3.38VPAALGTSILGSNGAAPSSGMYY93 pKa = 9.91LVPVKK98 pKa = 9.69EE99 pKa = 4.27TLNVVTDD106 pKa = 3.4HH107 pKa = 7.62AIAQFEE113 pKa = 4.42KK114 pKa = 10.71LQSAYY119 pKa = 10.22EE120 pKa = 4.0LDD122 pKa = 3.94RR123 pKa = 11.84DD124 pKa = 3.96YY125 pKa = 11.81LDD127 pKa = 3.99EE128 pKa = 6.08KK129 pKa = 11.05GVAPEE134 pKa = 4.02SVSFKK139 pKa = 11.22NYY141 pKa = 8.17LTYY144 pKa = 10.37VDD146 pKa = 4.96CYY148 pKa = 10.04VGVSARR154 pKa = 11.84QAATNFQKK162 pKa = 10.59HH163 pKa = 5.05VPVVTKK169 pKa = 10.61SKK171 pKa = 9.88MMSFMTSAQNILSLLGPWEE190 pKa = 3.87KK191 pKa = 10.6SIRR194 pKa = 11.84EE195 pKa = 4.29VLTMLPSSTPYY206 pKa = 9.59GTLKK210 pKa = 10.68CDD212 pKa = 3.28MKK214 pKa = 11.18AIVAMLEE221 pKa = 4.01EE222 pKa = 4.3QLPEE226 pKa = 4.29GNLCRR231 pKa = 11.84LYY233 pKa = 10.07PQAAACSIARR243 pKa = 11.84RR244 pKa = 11.84NGGVRR249 pKa = 11.84WKK251 pKa = 10.49EE252 pKa = 4.0PNSDD256 pKa = 3.92EE257 pKa = 4.79APSLATNDD265 pKa = 3.28VAASTMGALANTTPLADD282 pKa = 3.49KK283 pKa = 11.24SNTTEE288 pKa = 3.28EE289 pKa = 4.19SMRR292 pKa = 11.84LVSEE296 pKa = 4.25SSVDD300 pKa = 4.33LICSRR305 pKa = 11.84RR306 pKa = 11.84PISASVWARR315 pKa = 11.84TVEE318 pKa = 3.96PKK320 pKa = 10.41SYY322 pKa = 10.8NIRR325 pKa = 11.84TLKK328 pKa = 10.53VSEE331 pKa = 4.31ALWLRR336 pKa = 11.84QSQVSAGFDD345 pKa = 3.12VAYY348 pKa = 10.49NLNDD352 pKa = 3.08STQRR356 pKa = 11.84HH357 pKa = 4.9FWITTGTTVINLEE370 pKa = 4.19QTGSMMFEE378 pKa = 4.1VNIEE382 pKa = 3.87GKK384 pKa = 9.85DD385 pKa = 3.54YY386 pKa = 11.26KK387 pKa = 10.76KK388 pKa = 11.21GSFQPNGATLVLLVMQSRR406 pKa = 11.84LPFEE410 pKa = 4.04TWTVSSQIEE419 pKa = 4.7GIAQVASIVVTAGSGSTVNTSIIGSTSLSYY449 pKa = 10.94LFEE452 pKa = 6.11RR453 pKa = 11.84EE454 pKa = 4.28TITTANTEE462 pKa = 4.05VNTYY466 pKa = 9.95LLCTWQSSSVCTDD479 pKa = 3.0ADD481 pKa = 3.72SWPDD485 pKa = 2.79AWDD488 pKa = 3.34AVTTLTPLTTGTVTVKK504 pKa = 10.51GSTVEE509 pKa = 3.93KK510 pKa = 9.36VTPVDD515 pKa = 4.81LIGSYY520 pKa = 9.24TPEE523 pKa = 4.28SLTAALPNDD532 pKa = 4.02AGNILALRR540 pKa = 11.84AEE542 pKa = 4.42TLAKK546 pKa = 10.1AVKK549 pKa = 10.6NEE551 pKa = 4.09DD552 pKa = 3.7DD553 pKa = 3.9SVTDD557 pKa = 3.61EE558 pKa = 4.72SSPFSAPIQGILALQQKK575 pKa = 6.91EE576 pKa = 4.66TEE578 pKa = 4.47GTAGTPDD585 pKa = 4.04PKK587 pKa = 10.88PPGTLQKK594 pKa = 9.56IASRR598 pKa = 11.84AMHH601 pKa = 6.47MFLGDD606 pKa = 3.61PKK608 pKa = 10.98SVLKK612 pKa = 9.21QTTPVLKK619 pKa = 10.44DD620 pKa = 3.36PQVWTSFVQGVRR632 pKa = 11.84DD633 pKa = 4.76GIRR636 pKa = 2.98

Molecular weight: 68.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A1CFP6|A0A0A1CFP6_9REOV 1A (Fragment) OS=Pteropine orthoreovirus OX=1459003 PE=4 SV=1
MM1 pKa = 7.66SGDD4 pKa = 3.58CAKK7 pKa = 10.14IVSVFGSVHH16 pKa = 5.7CQSAKK21 pKa = 10.56NSAGGDD27 pKa = 3.61LQATSVFTTYY37 pKa = 9.34WPHH40 pKa = 5.75FAIGGGIIVIILLLGLFYY58 pKa = 10.93CCYY61 pKa = 10.28LKK63 pKa = 10.55WKK65 pKa = 8.3TSQVKK70 pKa = 8.08RR71 pKa = 11.84TYY73 pKa = 10.13RR74 pKa = 11.84RR75 pKa = 11.84EE76 pKa = 4.04LIALTRR82 pKa = 11.84SHH84 pKa = 6.04VHH86 pKa = 6.23PASSGISHH94 pKa = 6.41VV95 pKa = 3.59

Molecular weight: 10.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

6882

95

1277

625.6

69.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.123 ± 0.508

1.584 ± 0.124

5.812 ± 0.259

3.502 ± 0.452

4.054 ± 0.294

4.693 ± 0.285

2.005 ± 0.303

4.577 ± 0.258

3.124 ± 0.213

10.273 ± 0.38

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.746 ± 0.318

3.909 ± 0.471

6.452 ± 0.312

3.851 ± 0.278

5.696 ± 0.451

10.506 ± 0.729

6.728 ± 0.436

7.774 ± 0.32

1.744 ± 0.121

2.848 ± 0.338
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski