Phyllobacterium sp. YR620

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Phyllobacterium; unclassified Phyllobacterium

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4392 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H0VLL3|A0A1H0VLL3_9RHIZ Prephenate dehydratase OS=Phyllobacterium sp. YR620 OX=1881066 GN=SAMN05428967_3496 PE=4 SV=1
MM1 pKa = 7.52SNSKK5 pKa = 10.48NNTYY9 pKa = 10.62NFHH12 pKa = 7.25PGGDD16 pKa = 3.73PSATADD22 pKa = 3.43ADD24 pKa = 3.72ASAVAAALALQLQGQAQGQLEE45 pKa = 4.3AQGQGQGQGQSQSQDD60 pKa = 3.1QHH62 pKa = 7.56SSSDD66 pKa = 3.34NSNWNGNGNLNGNGNLNGNGNLNANGNLNANLNEE100 pKa = 4.28SDD102 pKa = 3.41NHH104 pKa = 6.13NANTNTNTSDD114 pKa = 3.4TAVHH118 pKa = 6.07VDD120 pKa = 3.13VGVNLDD126 pKa = 4.01GYY128 pKa = 11.18LPTDD132 pKa = 3.52NDD134 pKa = 3.7FADD137 pKa = 4.85LDD139 pKa = 4.1LSHH142 pKa = 7.2TNFDD146 pKa = 3.5NMFITDD152 pKa = 3.58SGSINFDD159 pKa = 3.38PGNDD163 pKa = 3.35VHH165 pKa = 8.06FSDD168 pKa = 4.95IFNGAFNGAGTNTGFAINQVADD190 pKa = 4.2LVDD193 pKa = 3.9NDD195 pKa = 3.91QLSHH199 pKa = 6.52VSQDD203 pKa = 3.04NSGSVTLDD211 pKa = 3.13ATGGAATAGDD221 pKa = 4.68GIGSGADD228 pKa = 3.42GWDD231 pKa = 3.13SWFGNHH237 pKa = 6.6GGGAHH242 pKa = 7.02AGDD245 pKa = 4.56DD246 pKa = 4.02VAGSSAASAAANLTSTAFTQDD267 pKa = 2.4IVLGANLQQNAFDD280 pKa = 3.52ATVINGTYY288 pKa = 8.35THH290 pKa = 6.0TTISGDD296 pKa = 3.64SEE298 pKa = 4.27

Molecular weight: 30.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H0VUD7|A0A1H0VUD7_9RHIZ TRAP-type C4-dicarboxylate transport system small permease component OS=Phyllobacterium sp. YR620 OX=1881066 GN=SAMN05428967_3679 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.43LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAGGRR28 pKa = 11.84KK29 pKa = 9.15VIAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4392

0

4392

1371109

25

2877

312.2

34.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.561 ± 0.043

0.757 ± 0.012

5.752 ± 0.036

5.68 ± 0.034

3.961 ± 0.027

8.303 ± 0.039

2.021 ± 0.018

5.943 ± 0.025

4.017 ± 0.027

9.832 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.512 ± 0.016

3.09 ± 0.024

4.921 ± 0.025

3.099 ± 0.018

6.396 ± 0.037

5.74 ± 0.026

5.432 ± 0.025

7.285 ± 0.033

1.285 ± 0.015

2.415 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski