Mycobacterium phage PG1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; Pegunavirus; Mycobacterium virus Pg1

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q716L2|Q716L2_9CAUD Uncharacterized protein OS=Mycobacterium phage PG1 OX=213236 GN=63 PE=4 SV=1
MM1 pKa = 6.88FTDD4 pKa = 3.48PRR6 pKa = 11.84IRR8 pKa = 11.84SAFLAGVAAGAALGVTVGAALVLTALPDD36 pKa = 3.92DD37 pKa = 4.34VSVPRR42 pKa = 11.84PGLSVTEE49 pKa = 4.16TAATKK54 pKa = 10.15QFAMWPADD62 pKa = 3.56PAPTPEE68 pKa = 5.48DD69 pKa = 4.68DD70 pKa = 3.44PTFDD74 pKa = 4.07CRR76 pKa = 11.84YY77 pKa = 9.18HH78 pKa = 6.77GNHH81 pKa = 5.92ICGPLNVDD89 pKa = 4.11GYY91 pKa = 11.9APGLYY96 pKa = 9.09IDD98 pKa = 4.99GVLVDD103 pKa = 4.79PWPVDD108 pKa = 3.43PASTPTIEE116 pKa = 4.27VPP118 pKa = 3.66

Molecular weight: 12.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q716K0|Q716K0_9CAUD Uncharacterized protein OS=Mycobacterium phage PG1 OX=213236 GN=75 PE=4 SV=1
MM1 pKa = 7.48ARR3 pKa = 11.84ATRR6 pKa = 11.84ARR8 pKa = 11.84GTRR11 pKa = 11.84SRR13 pKa = 11.84LGRR16 pKa = 11.84GSARR20 pKa = 11.84IARR23 pKa = 11.84SRR25 pKa = 11.84QRR27 pKa = 11.84GTISMSAATGGVGQFAKK44 pKa = 10.51RR45 pKa = 11.84GGRR48 pKa = 11.84RR49 pKa = 11.84GGGYY53 pKa = 10.09RR54 pKa = 11.84GFKK57 pKa = 10.23SKK59 pKa = 10.67KK60 pKa = 5.54QWRR63 pKa = 11.84WAWATKK69 pKa = 9.83QPWARR74 pKa = 11.84KK75 pKa = 8.71KK76 pKa = 10.09AHH78 pKa = 5.19EE79 pKa = 4.31TKK81 pKa = 9.92GGKK84 pKa = 7.0VVRR87 pKa = 11.84YY88 pKa = 9.47RR89 pKa = 11.84RR90 pKa = 11.84LPEE93 pKa = 4.35SKK95 pKa = 10.31HH96 pKa = 5.36SGHH99 pKa = 7.0RR100 pKa = 11.84GRR102 pKa = 11.84RR103 pKa = 11.84GPSRR107 pKa = 11.84KK108 pKa = 9.63GG109 pKa = 3.11

Molecular weight: 12.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

21647

45

1932

216.5

23.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.21 ± 0.381

1.127 ± 0.147

6.537 ± 0.228

5.871 ± 0.333

2.31 ± 0.119

9.618 ± 0.65

2.01 ± 0.193

4.62 ± 0.159

2.878 ± 0.14

7.923 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.07 ± 0.104

2.827 ± 0.212

6.126 ± 0.246

3.067 ± 0.126

6.929 ± 0.354

4.966 ± 0.203

7.054 ± 0.191

7.502 ± 0.192

1.862 ± 0.159

2.495 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski