Candidatus Babela massiliensis

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Candidatus Dependentiae; Candidatus Babeliae; Candidatus Babeliales; Candidatus Babeliaceae; Candidatus Babela

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 981 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V6DJL4|V6DJL4_9BACT Tfp pilus assembly protein ATPase PilM OS=Candidatus Babela massiliensis OX=673862 GN=BABL1_gene_309 PE=4 SV=1
MM1 pKa = 7.24NKK3 pKa = 9.74KK4 pKa = 10.2FLFLLIALPTIPACFWRR21 pKa = 11.84RR22 pKa = 11.84SPVNNCIVSEE32 pKa = 4.01PAPVEE37 pKa = 3.82IVEE40 pKa = 4.08PCNDD44 pKa = 3.1YY45 pKa = 10.93KK46 pKa = 11.6YY47 pKa = 9.88NAEE50 pKa = 4.1VQSYY54 pKa = 9.28QPSCQDD60 pKa = 3.08TQYY63 pKa = 11.58VNLAPEE69 pKa = 4.41TQCFDD74 pKa = 3.76NLVEE78 pKa = 4.48ADD80 pKa = 3.66EE81 pKa = 4.52YY82 pKa = 11.16EE83 pKa = 4.35YY84 pKa = 11.13EE85 pKa = 4.11PLVAEE90 pKa = 4.3SHH92 pKa = 6.69DD93 pKa = 4.49LGYY96 pKa = 10.85LYY98 pKa = 10.26QDD100 pKa = 4.05EE101 pKa = 5.18DD102 pKa = 3.9DD103 pKa = 4.25CDD105 pKa = 3.73EE106 pKa = 4.48YY107 pKa = 11.13EE108 pKa = 4.39YY109 pKa = 10.85EE110 pKa = 4.44YY111 pKa = 11.36ADD113 pKa = 3.71EE114 pKa = 5.82DD115 pKa = 4.28DD116 pKa = 4.83YY117 pKa = 12.17EE118 pKa = 4.23NAPIEE123 pKa = 4.18NVEE126 pKa = 4.03YY127 pKa = 10.65KK128 pKa = 10.5ADD130 pKa = 3.76YY131 pKa = 10.71GDD133 pKa = 5.09DD134 pKa = 4.02YY135 pKa = 11.49EE136 pKa = 4.34PQYY139 pKa = 11.76ANQEE143 pKa = 4.17DD144 pKa = 4.5AQDD147 pKa = 3.57LDD149 pKa = 3.9YY150 pKa = 10.96TQTEE154 pKa = 4.36EE155 pKa = 4.3EE156 pKa = 4.75LEE158 pKa = 3.91NDD160 pKa = 3.84YY161 pKa = 11.45EE162 pKa = 4.4QDD164 pKa = 3.09QDD166 pKa = 4.17NNDD169 pKa = 3.52HH170 pKa = 6.67EE171 pKa = 5.02EE172 pKa = 3.93EE173 pKa = 4.28FEE175 pKa = 4.12NDD177 pKa = 3.23EE178 pKa = 4.98QEE180 pKa = 6.07DD181 pKa = 3.85SDD183 pKa = 4.86EE184 pKa = 5.95DD185 pKa = 4.39DD186 pKa = 4.93DD187 pKa = 6.76LEE189 pKa = 6.24LGDD192 pKa = 4.35LAEE195 pKa = 4.48LEE197 pKa = 4.24EE198 pKa = 4.95PGFKK202 pKa = 10.41EE203 pKa = 4.33IIFNFNEE210 pKa = 4.08KK211 pKa = 10.04EE212 pKa = 3.95IKK214 pKa = 10.13NDD216 pKa = 3.34QEE218 pKa = 4.4EE219 pKa = 4.29MLKK222 pKa = 11.07NNLGKK227 pKa = 10.11IKK229 pKa = 10.22QLALSGHH236 pKa = 6.15KK237 pKa = 10.01IVVEE241 pKa = 4.01GHH243 pKa = 6.76ANQTPDD249 pKa = 4.51SEE251 pKa = 5.23DD252 pKa = 3.4NNLTISRR259 pKa = 11.84QRR261 pKa = 11.84AEE263 pKa = 4.04SVANYY268 pKa = 9.58LVQNGIDD275 pKa = 3.51QEE277 pKa = 4.69DD278 pKa = 3.88IQILAHH284 pKa = 6.11GSKK287 pKa = 9.62MPLIEE292 pKa = 4.15EE293 pKa = 4.17NGIEE297 pKa = 4.22GQSLNSRR304 pKa = 11.84VEE306 pKa = 3.72IYY308 pKa = 10.28SYY310 pKa = 11.51KK311 pKa = 10.25EE312 pKa = 3.52AA313 pKa = 5.32

Molecular weight: 36.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V6DFB9|V6DFB9_9BACT Ankyrin repeats containing protein OS=Candidatus Babela massiliensis OX=673862 GN=ankX_10 PE=4 SV=1
MM1 pKa = 7.82SITFKK6 pKa = 10.94LKK8 pKa = 9.45RR9 pKa = 11.84KK10 pKa = 8.98KK11 pKa = 10.21RR12 pKa = 11.84NRR14 pKa = 11.84KK15 pKa = 8.84HH16 pKa = 6.31GFLKK20 pKa = 10.64RR21 pKa = 11.84MATHH25 pKa = 7.58DD26 pKa = 2.99GRR28 pKa = 11.84KK29 pKa = 7.91IVKK32 pKa = 9.34RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ASGRR39 pKa = 11.84KK40 pKa = 9.1RR41 pKa = 11.84IAVHH45 pKa = 6.34AA46 pKa = 4.58

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

981

0

981

326148

34

2683

332.5

37.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.251 ± 0.065

1.113 ± 0.034

5.297 ± 0.057

5.96 ± 0.101

4.887 ± 0.075

4.58 ± 0.1

1.545 ± 0.032

10.218 ± 0.113

8.942 ± 0.136

11.002 ± 0.107

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.764 ± 0.032

7.798 ± 0.111

2.827 ± 0.054

3.681 ± 0.054

3.169 ± 0.066

7.124 ± 0.127

4.883 ± 0.119

4.98 ± 0.081

0.764 ± 0.023

4.215 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski