Giant house spider associated circular virus 4

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346BPE6|A0A346BPE6_9VIRU Coat protein OS=Giant house spider associated circular virus 4 OX=2293291 PE=4 SV=1
MM1 pKa = 7.61SFRR4 pKa = 11.84FNARR8 pKa = 11.84AVFLTYY14 pKa = 9.04PQCPLLKK21 pKa = 10.05QHH23 pKa = 6.15VLTMLRR29 pKa = 11.84TKK31 pKa = 10.65LGEE34 pKa = 4.35DD35 pKa = 3.4EE36 pKa = 4.28GTHH39 pKa = 5.17YY40 pKa = 11.19LIGQEE45 pKa = 4.09THH47 pKa = 7.17KK48 pKa = 11.11DD49 pKa = 3.74GTPHH53 pKa = 6.46LHH55 pKa = 6.05IFVEE59 pKa = 4.61RR60 pKa = 11.84GHH62 pKa = 7.38LINTRR67 pKa = 11.84NARR70 pKa = 11.84YY71 pKa = 9.21FDD73 pKa = 4.74LEE75 pKa = 4.32DD76 pKa = 3.71ASGIYY81 pKa = 9.34HH82 pKa = 6.95PNVTAPRR89 pKa = 11.84DD90 pKa = 3.42RR91 pKa = 11.84ADD93 pKa = 3.46VIRR96 pKa = 11.84YY97 pKa = 5.85ITKK100 pKa = 10.29EE101 pKa = 3.6DD102 pKa = 3.75TEE104 pKa = 4.29PVKK107 pKa = 10.55WPEE110 pKa = 3.35TWKK113 pKa = 10.6FDD115 pKa = 3.73EE116 pKa = 4.83PPKK119 pKa = 10.61KK120 pKa = 9.71RR121 pKa = 11.84SKK123 pKa = 10.04WEE125 pKa = 3.51QATPLLLEE133 pKa = 4.66GQDD136 pKa = 3.3AATLLKK142 pKa = 10.74SMPVFVLGNLKK153 pKa = 9.96KK154 pKa = 10.53VQEE157 pKa = 4.28ATAFLANIKK166 pKa = 9.27QQASLPPLEE175 pKa = 5.07TFLTWVLPAEE185 pKa = 4.38PEE187 pKa = 4.14YY188 pKa = 10.97EE189 pKa = 3.86RR190 pKa = 11.84NIAFKK195 pKa = 9.51TVWNEE200 pKa = 3.5LRR202 pKa = 11.84DD203 pKa = 3.91NLASKK208 pKa = 10.95DD209 pKa = 3.47FGRR212 pKa = 11.84RR213 pKa = 11.84QLYY216 pKa = 9.78LHH218 pKa = 6.95GPTGIGKK225 pKa = 8.25STFLRR230 pKa = 11.84HH231 pKa = 6.51LLMCIRR237 pKa = 11.84TYY239 pKa = 10.77ILPNEE244 pKa = 4.28DD245 pKa = 5.16WYY247 pKa = 10.75DD248 pKa = 3.17HH249 pKa = 6.59WDD251 pKa = 3.51NNLFDD256 pKa = 4.81LSVMEE261 pKa = 4.32EE262 pKa = 4.02FKK264 pKa = 10.89GQKK267 pKa = 9.61SIQWLNQWLDD277 pKa = 3.35EE278 pKa = 3.81AHH280 pKa = 6.79FYY282 pKa = 10.96VKK284 pKa = 10.42RR285 pKa = 11.84KK286 pKa = 9.61GVAGLLKK293 pKa = 9.78TNPIPTILISNFDD306 pKa = 3.77INSSDD311 pKa = 3.75VYY313 pKa = 11.23PNMQEE318 pKa = 4.06SVSIQTLRR326 pKa = 11.84RR327 pKa = 11.84RR328 pKa = 11.84LRR330 pKa = 11.84SLPVDD335 pKa = 3.81STCMHH340 pKa = 7.64LLTKK344 pKa = 10.49ALRR347 pKa = 11.84QFLSSIGASLPHH359 pKa = 6.36IQGDD363 pKa = 4.27VPQTPPLNAAQEE375 pKa = 4.38IVNPLWRR382 pKa = 11.84GPEE385 pKa = 3.92VTTVLPAPPVGGQTEE400 pKa = 4.48HH401 pKa = 5.95TCYY404 pKa = 10.08GQNGAPFRR412 pKa = 11.84DD413 pKa = 3.39EE414 pKa = 5.16CGPSTCNAWHH424 pKa = 6.65

Molecular weight: 48.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346BPE6|A0A346BPE6_9VIRU Coat protein OS=Giant house spider associated circular virus 4 OX=2293291 PE=4 SV=1
MM1 pKa = 7.81PKK3 pKa = 9.93RR4 pKa = 11.84RR5 pKa = 11.84LTYY8 pKa = 10.66DD9 pKa = 3.19SVKK12 pKa = 10.96DD13 pKa = 3.63MPLSKK18 pKa = 10.16LKK20 pKa = 10.74KK21 pKa = 8.96VMRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84NRR28 pKa = 11.84APRR31 pKa = 11.84SYY33 pKa = 10.9RR34 pKa = 11.84SGARR38 pKa = 11.84VKK40 pKa = 10.97GSIARR45 pKa = 11.84YY46 pKa = 9.43GGRR49 pKa = 11.84QEE51 pKa = 4.95LKK53 pKa = 10.95YY54 pKa = 10.95VDD56 pKa = 3.64TDD58 pKa = 3.81LTGGTALYY66 pKa = 7.58DD67 pKa = 3.75TTGLATPVNLLAIGDD82 pKa = 4.54DD83 pKa = 3.45NTSRR87 pKa = 11.84DD88 pKa = 3.41GRR90 pKa = 11.84QVTVKK95 pKa = 10.24SIQVQGSVFPVDD107 pKa = 3.46QGAGPTLCRR116 pKa = 11.84SMIVWDD122 pKa = 4.14AMNNSASTTSAQLIAALLQASTSNAFPLIDD152 pKa = 3.44NQQRR156 pKa = 11.84FTVLWDD162 pKa = 3.23SHH164 pKa = 7.71KK165 pKa = 10.72MLGQISNTATQALSPNPGAHH185 pKa = 5.79ILKK188 pKa = 9.65YY189 pKa = 9.69YY190 pKa = 10.59RR191 pKa = 11.84KK192 pKa = 8.97ISQVTQYY199 pKa = 11.08SGTTAAIGSIQSGALWFVTLGDD221 pKa = 3.47NAAGVGGQFIGRR233 pKa = 11.84VRR235 pKa = 11.84VRR237 pKa = 11.84FTDD240 pKa = 3.23NN241 pKa = 2.94

Molecular weight: 26.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

665

241

424

332.5

37.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.068 ± 1.261

1.053 ± 0.39

5.263 ± 0.334

4.06 ± 2.23

3.609 ± 0.685

6.767 ± 1.445

2.556 ± 1.056

4.812 ± 0.102

4.962 ± 0.244

10.226 ± 1.179

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.955 ± 0.327

4.812 ± 0.152

6.165 ± 1.487

5.263 ± 0.588

6.466 ± 0.867

6.165 ± 1.559

7.82 ± 0.547

5.865 ± 0.728

2.105 ± 0.526

3.008 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski