Desulfosarcina widdelii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; Desulfosarcina

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6679 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5K7Z4E5|A0A5K7Z4E5_9DELT Uncharacterized protein OS=Desulfosarcina widdelii OX=947919 GN=DSCW_32750 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.27KK3 pKa = 10.43VSLLIALCSALMIAAPVMALEE24 pKa = 4.66VEE26 pKa = 4.6VSGHH30 pKa = 5.3YY31 pKa = 9.74FVEE34 pKa = 4.99TYY36 pKa = 11.23NNSNSNLSPNDD47 pKa = 3.29ATDD50 pKa = 4.79DD51 pKa = 3.78YY52 pKa = 12.05SKK54 pKa = 10.48MEE56 pKa = 4.23FMAKK60 pKa = 9.66PVFKK64 pKa = 10.01ITDD67 pKa = 4.21NITLTTQFTGLEE79 pKa = 4.02GHH81 pKa = 6.09VWGDD85 pKa = 3.52NPSDD89 pKa = 3.67AGSEE93 pKa = 4.27FDD95 pKa = 5.32DD96 pKa = 5.2DD97 pKa = 4.69YY98 pKa = 12.23NNIEE102 pKa = 3.89WKK104 pKa = 9.87AAYY107 pKa = 7.5MTIKK111 pKa = 9.79TPIGGFIVGRR121 pKa = 11.84YY122 pKa = 9.01IDD124 pKa = 4.44TPWGTDD130 pKa = 3.23LGDD133 pKa = 3.53STASHH138 pKa = 6.96GSNDD142 pKa = 3.51LAKK145 pKa = 10.68DD146 pKa = 3.61RR147 pKa = 11.84IMWVVPVGDD156 pKa = 5.0FISGLVYY163 pKa = 10.49QRR165 pKa = 11.84VGEE168 pKa = 4.08GDD170 pKa = 3.47KK171 pKa = 11.26GVSDD175 pKa = 3.81SDD177 pKa = 3.35KK178 pKa = 11.55DD179 pKa = 3.7NIRR182 pKa = 11.84AYY184 pKa = 10.67GFTAYY189 pKa = 9.53KK190 pKa = 9.26QEE192 pKa = 3.84NWSTGLLVSRR202 pKa = 11.84YY203 pKa = 7.27NLKK206 pKa = 10.89GFVDD210 pKa = 3.9MADD213 pKa = 3.51LRR215 pKa = 11.84ALQAGYY221 pKa = 11.24NNYY224 pKa = 9.25EE225 pKa = 4.22ALNDD229 pKa = 3.89AATLAQATYY238 pKa = 10.43DD239 pKa = 3.37GAYY242 pKa = 8.6AQIYY246 pKa = 10.1AGLAPLLGEE255 pKa = 4.38DD256 pKa = 3.53AAYY259 pKa = 10.21AAIQGEE265 pKa = 4.31LAATNDD271 pKa = 3.47FALGQAVDD279 pKa = 4.04LANLQRR285 pKa = 11.84DD286 pKa = 3.46ASLAAGQAAAAGSLISGGLTNSTAAFWVLDD316 pKa = 4.17PYY318 pKa = 11.35FKK320 pKa = 10.76GTFGPLGIEE329 pKa = 4.3AEE331 pKa = 4.05FLYY334 pKa = 11.01AFGTLEE340 pKa = 4.19LDD342 pKa = 3.54EE343 pKa = 4.75TRR345 pKa = 11.84TDD347 pKa = 3.23VRR349 pKa = 11.84NGSQFDD355 pKa = 3.97EE356 pKa = 4.73LDD358 pKa = 3.99GEE360 pKa = 4.5AMAATIDD367 pKa = 3.87LKK369 pKa = 10.4YY370 pKa = 9.13TIAGFTFNGGYY381 pKa = 8.96TYY383 pKa = 11.22VQGDD387 pKa = 4.12SDD389 pKa = 3.67WADD392 pKa = 3.7DD393 pKa = 3.87EE394 pKa = 4.89FGSIGYY400 pKa = 9.52LEE402 pKa = 3.91QSIDD406 pKa = 3.79LEE408 pKa = 4.57HH409 pKa = 6.89GFLLTSDD416 pKa = 3.67TADD419 pKa = 3.2MEE421 pKa = 4.8GTFGGTDD428 pKa = 3.16ANGIALGNLAGGRR441 pKa = 11.84QTLAGCAGYY450 pKa = 10.96QMYY453 pKa = 9.79WLGAEE458 pKa = 4.16YY459 pKa = 10.3QVLDD463 pKa = 3.78NLKK466 pKa = 9.96IGALFVSSKK475 pKa = 11.29ADD477 pKa = 3.52DD478 pKa = 4.16APYY481 pKa = 11.14LDD483 pKa = 4.41LTSTDD488 pKa = 3.32KK489 pKa = 11.24EE490 pKa = 4.27QWDD493 pKa = 3.98DD494 pKa = 3.92DD495 pKa = 4.0HH496 pKa = 7.85GQEE499 pKa = 4.72YY500 pKa = 10.96DD501 pKa = 4.88LTVEE505 pKa = 4.0WNIMDD510 pKa = 4.37NLTFNGVVAHH520 pKa = 6.76LAAGDD525 pKa = 3.57FWKK528 pKa = 10.95AGDD531 pKa = 4.1PDD533 pKa = 5.41AEE535 pKa = 4.51IEE537 pKa = 4.86DD538 pKa = 3.62NTTFYY543 pKa = 11.13GQLIVEE549 pKa = 4.79FF550 pKa = 4.61

Molecular weight: 59.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5K7ZFJ8|A0A5K7ZFJ8_9DELT 2-dehydropantoate 2-reductase OS=Desulfosarcina widdelii OX=947919 GN=DSCW_59100 PE=3 SV=1
MM1 pKa = 7.5LLAGALAIPFHH12 pKa = 6.68FEE14 pKa = 4.36SPSMWYY20 pKa = 10.28KK21 pKa = 10.75FGAAKK26 pKa = 10.21ISLRR30 pKa = 11.84AGKK33 pKa = 8.61MLGMAAGLMILLQLPLAGRR52 pKa = 11.84LKK54 pKa = 10.83LLDD57 pKa = 5.09RR58 pKa = 11.84IFSLPGLIRR67 pKa = 11.84QHH69 pKa = 6.35RR70 pKa = 11.84WHH72 pKa = 6.83AWAIVIMALIHH83 pKa = 5.75PLCVLLAEE91 pKa = 4.95GKK93 pKa = 10.45LLIPLEE99 pKa = 3.98MRR101 pKa = 11.84YY102 pKa = 8.33WPEE105 pKa = 3.38WVGVGLLVVLLAQFACTQWRR125 pKa = 11.84RR126 pKa = 11.84PLKK129 pKa = 10.31IAFHH133 pKa = 6.52HH134 pKa = 6.77WLTGHH139 pKa = 7.38RR140 pKa = 11.84IAGLITAALLIVHH153 pKa = 5.83VRR155 pKa = 11.84YY156 pKa = 10.31VSEE159 pKa = 4.36TFTDD163 pKa = 3.68NPPPRR168 pKa = 11.84LAVLSAAGVFTLFWLWIRR186 pKa = 11.84STWLRR191 pKa = 11.84TRR193 pKa = 11.84RR194 pKa = 11.84TPFSVTRR201 pKa = 11.84VEE203 pKa = 4.54NIGRR207 pKa = 11.84EE208 pKa = 4.4CTCVEE213 pKa = 3.97VAPEE217 pKa = 3.9KK218 pKa = 10.14GAAMAYY224 pKa = 10.3FPGQFAFVSFTSQILSRR241 pKa = 11.84EE242 pKa = 3.99PHH244 pKa = 6.73PFTLASTPSRR254 pKa = 11.84PGTIQFAIRR263 pKa = 11.84HH264 pKa = 5.73CGDD267 pKa = 2.63WTRR270 pKa = 11.84QVEE273 pKa = 4.27TLAIGDD279 pKa = 3.73RR280 pKa = 11.84AYY282 pKa = 11.13LQGPFGRR289 pKa = 11.84FGHH292 pKa = 7.29LLQASGQQEE301 pKa = 4.94LIMIAGGIGITPMLSMLRR319 pKa = 11.84FMADD323 pKa = 2.59HH324 pKa = 7.18GDD326 pKa = 3.65PRR328 pKa = 11.84PITLIWSNRR337 pKa = 11.84TRR339 pKa = 11.84TQLVFTEE346 pKa = 4.81EE347 pKa = 4.12FQHH350 pKa = 7.19LSAKK354 pKa = 9.2LTGLRR359 pKa = 11.84RR360 pKa = 11.84VPIFTEE366 pKa = 4.22NADD369 pKa = 3.37SGTPSGRR376 pKa = 11.84LNRR379 pKa = 11.84IMLQSLLSGCSRR391 pKa = 11.84GSAVFLCGPPSMMTALTTEE410 pKa = 4.68LKK412 pKa = 10.89ALGFSARR419 pKa = 11.84LIITEE424 pKa = 4.11TFGFF428 pKa = 4.04

Molecular weight: 47.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6679

0

6679

2063912

39

2590

309.0

34.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.09 ± 0.036

1.297 ± 0.014

5.831 ± 0.025

6.228 ± 0.026

4.189 ± 0.02

7.583 ± 0.026

2.07 ± 0.013

6.528 ± 0.028

5.258 ± 0.028

9.661 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.814 ± 0.015

3.436 ± 0.016

4.514 ± 0.02

3.359 ± 0.016

6.202 ± 0.026

5.572 ± 0.02

5.241 ± 0.017

7.108 ± 0.025

1.16 ± 0.014

2.858 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski