Ectothiorhodospiraceae bacterium BW-2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; unclassified Ectothiorhodospiraceae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3445 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A5C2HU83|A0A5C2HU83_9GAMM Methyl-accepting chemotaxis protein OS=Ectothiorhodospiraceae bacterium BW-2 OX=947515 GN=D5085_15465 PE=4 SV=1
MM1 pKa = 6.61TQVTATTDD9 pKa = 3.15SSSVSFDD16 pKa = 3.03EE17 pKa = 4.26TAPALTAVSIASDD30 pKa = 3.51NSDD33 pKa = 3.06TTLAKK38 pKa = 10.13TGDD41 pKa = 3.74TVTLSFTTDD50 pKa = 3.17GSHH53 pKa = 6.86SGTPTVTLGGNGVTATNTSGNTYY76 pKa = 7.73TASYY80 pKa = 8.85TLQAGDD86 pKa = 3.5TEE88 pKa = 4.82GAVSFTIDD96 pKa = 3.36AVDD99 pKa = 3.24AAGNAMTQVTATTDD113 pKa = 3.15SSSVSFDD120 pKa = 3.03EE121 pKa = 4.26TAPALTAVSIASDD134 pKa = 3.5NSDD137 pKa = 3.11TALAKK142 pKa = 10.19TGDD145 pKa = 3.77TVTLSFTTDD154 pKa = 3.17GSHH157 pKa = 6.86SGTPTVTLGGNSVTATNTSGNTYY180 pKa = 7.73TASYY184 pKa = 8.85TLQAGDD190 pKa = 3.5TEE192 pKa = 4.82GAVSFTIDD200 pKa = 3.36AVDD203 pKa = 3.24AAGNAMTQVTATTDD217 pKa = 3.15SSSVSFDD224 pKa = 3.03EE225 pKa = 4.26TAPALTAVSIASDD238 pKa = 3.51NSDD241 pKa = 3.08TTLAKK246 pKa = 10.86VGDD249 pKa = 4.21TVTLSFTTDD258 pKa = 3.17GSHH261 pKa = 6.86SGTPTVTLGGNGVTATNTSGNTYY284 pKa = 7.73TASYY288 pKa = 8.85TLQAGDD294 pKa = 3.5TEE296 pKa = 4.82GAVSFTIDD304 pKa = 3.36AVDD307 pKa = 3.24AAGNAMTQVTATTDD321 pKa = 3.15SSSVSFDD328 pKa = 3.03EE329 pKa = 4.26TAPALTAVSIASDD342 pKa = 3.51NSDD345 pKa = 3.08TTLAKK350 pKa = 10.86VGDD353 pKa = 4.21TVTLSFTTDD362 pKa = 3.17GSHH365 pKa = 6.86SGTPTVTLGGNSVTATNTSGNTYY388 pKa = 7.73TASYY392 pKa = 8.74TLQAGDD398 pKa = 3.61IEE400 pKa = 4.76GTLSFTIDD408 pKa = 3.19AVDD411 pKa = 3.24AAGNAMTQVTATTDD425 pKa = 3.15SSSVSFDD432 pKa = 3.03EE433 pKa = 4.26TAPALTAVSIASDD446 pKa = 3.51NSDD449 pKa = 3.08TTLAKK454 pKa = 10.86VGDD457 pKa = 4.21TVTLSFTTDD466 pKa = 3.17GSHH469 pKa = 6.86SGTPTVTLGGNSVTATNTSGNTYY492 pKa = 7.73TASYY496 pKa = 8.85TLQAGDD502 pKa = 3.5TEE504 pKa = 4.82GAVSFTIDD512 pKa = 3.36AVDD515 pKa = 3.24AAGNAMTQVTATTDD529 pKa = 3.15SSSVSFDD536 pKa = 3.03TTAPALTAVSIASDD550 pKa = 3.5NSDD553 pKa = 3.11TALAKK558 pKa = 10.19TGDD561 pKa = 3.77TVTLSFTTDD570 pKa = 3.17GSHH573 pKa = 6.86SGTPTVTLGGNGVTATNTSGNTYY596 pKa = 7.73TASYY600 pKa = 8.85TLQAGDD606 pKa = 3.5TEE608 pKa = 4.86GAVSFSIDD616 pKa = 3.24AVDD619 pKa = 3.4AAGNAMTQVTATTDD633 pKa = 3.15SSSVSFDD640 pKa = 3.03EE641 pKa = 4.26TAPALTAVSIASDD654 pKa = 3.51NSDD657 pKa = 3.08TTLAKK662 pKa = 10.86VGDD665 pKa = 4.21TVTLSFTTDD674 pKa = 3.17GSHH677 pKa = 6.86SGTPTVTLGGNSVTATNTSGNTYY700 pKa = 7.73TASYY704 pKa = 8.85TLQAGDD710 pKa = 3.5TEE712 pKa = 4.82GAVSFTIDD720 pKa = 3.36AVDD723 pKa = 3.24AAGNAMTQVTATTDD737 pKa = 3.15SSSVSFDD744 pKa = 3.03EE745 pKa = 4.17TAPTINSVSVADD757 pKa = 3.88GTYY760 pKa = 10.38KK761 pKa = 10.36IGDD764 pKa = 4.63AITITVTAGSNEE776 pKa = 4.2TGLTLAGTFNGQTLTGITDD795 pKa = 3.84NNDD798 pKa = 2.67GTYY801 pKa = 10.42SATYY805 pKa = 8.46TAVEE809 pKa = 4.29GDD811 pKa = 3.83SDD813 pKa = 4.02VADD816 pKa = 4.31AGSVTTNITLTDD828 pKa = 3.48AAGNASSATTSVTLAGEE845 pKa = 4.56SIDD848 pKa = 4.09GNSPTGSVSDD858 pKa = 4.19LVTFPTIQLEE868 pKa = 4.32ATGYY872 pKa = 5.74TTGEE876 pKa = 3.85DD877 pKa = 3.05RR878 pKa = 11.84YY879 pKa = 10.9VALEE883 pKa = 4.09SVGSDD888 pKa = 2.61GGYY891 pKa = 8.51VVAWEE896 pKa = 4.71GEE898 pKa = 4.28YY899 pKa = 11.1GSYY902 pKa = 9.47PLKK905 pKa = 10.6SIYY908 pKa = 9.67VQQFNADD915 pKa = 3.57GTTTGYY921 pKa = 10.95SPVQLDD927 pKa = 4.51GIGVTDD933 pKa = 3.76SYY935 pKa = 11.78DD936 pKa = 3.51YY937 pKa = 10.71RR938 pKa = 11.84PKK940 pKa = 9.49ITALEE945 pKa = 4.1SGGYY949 pKa = 8.53VVTWSGLDD957 pKa = 3.45DD958 pKa = 4.97SGDD961 pKa = 3.41NDD963 pKa = 3.52TSVFVQQFNADD974 pKa = 3.72GTTTGYY980 pKa = 11.28DD981 pKa = 3.6PVQLEE986 pKa = 4.28ATGYY990 pKa = 7.46TAGEE994 pKa = 4.17DD995 pKa = 3.97DD996 pKa = 4.35EE997 pKa = 4.75PQITSLGSSGEE1008 pKa = 4.04YY1009 pKa = 9.08VVIWRR1014 pKa = 11.84GQNSSTSSDD1023 pKa = 2.65RR1024 pKa = 11.84SIFVQQFNANGTVKK1038 pKa = 10.7NDD1040 pKa = 3.35TPVILEE1046 pKa = 4.08AHH1048 pKa = 6.66GSWDD1052 pKa = 3.3QYY1054 pKa = 9.09QQITTLNSGGYY1065 pKa = 8.41VVTWTGLDD1073 pKa = 3.3GTIYY1077 pKa = 10.4PDD1079 pKa = 3.4TDD1081 pKa = 3.29YY1082 pKa = 11.37SIYY1085 pKa = 10.3VQQFNADD1092 pKa = 3.59GTRR1095 pKa = 11.84TGDD1098 pKa = 3.51TPVKK1102 pKa = 10.83LEE1104 pKa = 4.01GTGNTTGGDD1113 pKa = 3.2EE1114 pKa = 4.3HH1115 pKa = 8.04SQVTALDD1122 pKa = 3.78FNGGYY1127 pKa = 8.93VVAWKK1132 pKa = 9.61GQNSDD1137 pKa = 3.14NRR1139 pKa = 11.84DD1140 pKa = 3.7SIFVQQFDD1148 pKa = 4.1ANGTITGYY1156 pKa = 9.07TPVQLNSHH1164 pKa = 6.08SNDD1167 pKa = 2.97RR1168 pKa = 11.84DD1169 pKa = 3.64VQIAAVGSSGQYY1181 pKa = 7.72VATWVGMDD1189 pKa = 3.59SNYY1192 pKa = 10.72SYY1194 pKa = 11.18DD1195 pKa = 3.48IFVQQFNASGTTDD1208 pKa = 2.73GFTTVKK1214 pKa = 10.5LEE1216 pKa = 4.54SINNPDD1222 pKa = 3.55SGNGAKK1228 pKa = 10.12PKK1230 pKa = 10.06IAALGDD1236 pKa = 3.34SGRR1239 pKa = 11.84YY1240 pKa = 8.63LVTWYY1245 pKa = 10.11DD1246 pKa = 3.4YY1247 pKa = 11.46DD1248 pKa = 3.92EE1249 pKa = 5.44EE1250 pKa = 6.28GDD1252 pKa = 3.61EE1253 pKa = 5.6SIFLQAFNADD1263 pKa = 3.45GTTAGPTQQFEE1274 pKa = 4.52ATGNTTGSDD1283 pKa = 3.45QYY1285 pKa = 11.55PEE1287 pKa = 4.03VLALGDD1293 pKa = 3.32SGAFVLAWHH1302 pKa = 6.61GRR1304 pKa = 11.84DD1305 pKa = 3.3SDD1307 pKa = 4.13DD1308 pKa = 4.51DD1309 pKa = 4.1VSIFVKK1315 pKa = 10.54QFFIDD1320 pKa = 3.65GMVRR1324 pKa = 11.84AQSSEE1329 pKa = 3.64AATAYY1334 pKa = 10.07LVKK1337 pKa = 10.27DD1338 pKa = 4.23TITVNSVTDD1347 pKa = 3.43LTGAPDD1353 pKa = 3.47SQMNTVAITSANTDD1367 pKa = 2.67TDD1369 pKa = 4.03LPLTGLEE1376 pKa = 4.03EE1377 pKa = 4.54GNYY1380 pKa = 9.07TLYY1383 pKa = 11.16LVDD1386 pKa = 3.34AAGNIGTVGTSVTVDD1401 pKa = 3.32HH1402 pKa = 6.97TPPNAPTISAVTDD1415 pKa = 3.65DD1416 pKa = 3.93QGSVTGTVADD1426 pKa = 5.16DD1427 pKa = 5.12GISDD1431 pKa = 3.93DD1432 pKa = 4.14TILTVRR1438 pKa = 11.84VSLDD1442 pKa = 3.17STNALSGDD1450 pKa = 3.82SVQLYY1455 pKa = 10.77DD1456 pKa = 4.16GFSALGTAVTLTSTDD1471 pKa = 2.73ISNGYY1476 pKa = 9.6VDD1478 pKa = 5.05IDD1480 pKa = 4.28TPTLTAGTSYY1490 pKa = 11.25NFRR1493 pKa = 11.84ANITDD1498 pKa = 3.19AFGNIGEE1505 pKa = 4.54FSTDD1509 pKa = 2.66GAADD1513 pKa = 3.56VTIDD1517 pKa = 3.27TTAPDD1522 pKa = 3.97GNVVSTTAFVTLEE1535 pKa = 3.96ASGVTNMDD1543 pKa = 3.57DD1544 pKa = 4.1RR1545 pKa = 11.84NPQISAIGSNGEE1557 pKa = 4.38YY1558 pKa = 10.25VVTWTGGDD1566 pKa = 3.7GPDD1569 pKa = 5.09DD1570 pKa = 4.79YY1571 pKa = 11.74DD1572 pKa = 4.47DD1573 pKa = 4.77SIYY1576 pKa = 10.67VQHH1579 pKa = 6.9FNTDD1583 pKa = 2.96GTITGKK1589 pKa = 10.48AQVKK1593 pKa = 10.24LEE1595 pKa = 4.26STGVTDD1601 pKa = 5.17GGDD1604 pKa = 3.38SAPQVTSVGISGEE1617 pKa = 4.49YY1618 pKa = 8.94VVTWVGNDD1626 pKa = 3.55GPDD1629 pKa = 4.22DD1630 pKa = 4.74YY1631 pKa = 11.75DD1632 pKa = 4.4DD1633 pKa = 4.77SIYY1636 pKa = 10.63VQHH1639 pKa = 6.62FNADD1643 pKa = 3.27GTITGKK1649 pKa = 10.51AQVKK1653 pKa = 10.24LEE1655 pKa = 4.24STGVTDD1661 pKa = 5.59RR1662 pKa = 11.84GDD1664 pKa = 3.37EE1665 pKa = 4.25APQVTSVGISGEE1677 pKa = 4.49YY1678 pKa = 8.94VVTWVGNDD1686 pKa = 3.46GPGDD1690 pKa = 4.06YY1691 pKa = 10.52DD1692 pKa = 4.01DD1693 pKa = 5.56SIYY1696 pKa = 10.67VQHH1699 pKa = 6.62FNADD1703 pKa = 3.27GTITGKK1709 pKa = 10.47AQVKK1713 pKa = 9.97LEE1715 pKa = 4.14PTGVTDD1721 pKa = 6.01RR1722 pKa = 11.84GDD1724 pKa = 3.57KK1725 pKa = 10.71DD1726 pKa = 3.66PQVTSVGISGEE1737 pKa = 4.49YY1738 pKa = 8.94VVTWVGNDD1746 pKa = 3.43GSDD1749 pKa = 3.04EE1750 pKa = 4.42SIYY1753 pKa = 10.16VQHH1756 pKa = 6.6FNADD1760 pKa = 3.34GTITGKK1766 pKa = 10.27DD1767 pKa = 3.47QVKK1770 pKa = 10.64LEE1772 pKa = 4.19PTGQLSGDD1780 pKa = 3.82DD1781 pKa = 4.34LRR1783 pKa = 11.84PQVSAVGSNGEE1794 pKa = 4.28YY1795 pKa = 9.75VVVWDD1800 pKa = 5.26GVDD1803 pKa = 3.24ADD1805 pKa = 3.91GDD1807 pKa = 3.54RR1808 pKa = 11.84SVFVQRR1814 pKa = 11.84FNADD1818 pKa = 2.9GTKK1821 pKa = 10.64NGTEE1825 pKa = 4.08VLLEE1829 pKa = 4.01ATGVTDD1835 pKa = 4.78QSDD1838 pKa = 3.92MQAKK1842 pKa = 9.24IAAIGSSGEE1851 pKa = 4.67FVVTWPGKK1859 pKa = 10.41DD1860 pKa = 3.11SDD1862 pKa = 4.17GDD1864 pKa = 3.62DD1865 pKa = 4.04SIFVQHH1871 pKa = 6.36FNADD1875 pKa = 3.41GSTTNHH1881 pKa = 6.79SIVQLEE1887 pKa = 4.6AIGVTDD1893 pKa = 5.72DD1894 pKa = 4.46RR1895 pKa = 11.84DD1896 pKa = 3.71EE1897 pKa = 4.56NPQITALANSGYY1909 pKa = 10.06VVTWLGDD1916 pKa = 3.46DD1917 pKa = 4.54ANNDD1921 pKa = 3.53DD1922 pKa = 5.3QVYY1925 pKa = 9.39LQQFNADD1932 pKa = 3.54GSKK1935 pKa = 10.27SGSVTLVEE1943 pKa = 4.27VGNNEE1948 pKa = 4.09IASKK1952 pKa = 10.5GYY1954 pKa = 9.81PEE1956 pKa = 4.03IAAVGIGSQFAITSRR1971 pKa = 11.84GDD1973 pKa = 3.27DD1974 pKa = 3.25SGGDD1978 pKa = 3.21DD1979 pKa = 3.23SVYY1982 pKa = 10.55VRR1984 pKa = 11.84QFDD1987 pKa = 3.52ASTVFNNSKK1996 pKa = 8.37TVQVQSDD2003 pKa = 4.75GPGMAYY2009 pKa = 10.6LIKK2012 pKa = 10.75DD2013 pKa = 3.57SVTINSAADD2022 pKa = 3.55FSGVTDD2028 pKa = 3.58SQINSVSITSANTFTDD2044 pKa = 3.58LTLSGLEE2051 pKa = 3.9AGDD2054 pKa = 3.58YY2055 pKa = 10.82KK2056 pKa = 11.2LYY2058 pKa = 11.01LVDD2061 pKa = 3.53AAGNLGITTDD2071 pKa = 4.47GLTIDD2076 pKa = 4.0NTAPTASVTAATIGSSGNAVVQSTEE2101 pKa = 3.47AGTAFLVKK2109 pKa = 10.57DD2110 pKa = 4.18SITVNSLSDD2119 pKa = 3.13ITGASDD2125 pKa = 5.1NNWNWVTISTASSDD2139 pKa = 3.95TNLAAAGLVDD2149 pKa = 4.58GDD2151 pKa = 4.11YY2152 pKa = 11.05KK2153 pKa = 11.51VYY2155 pKa = 8.47TTDD2158 pKa = 3.17SAGNLSTVSTNSVTIDD2174 pKa = 3.35TTPPTTTYY2182 pKa = 10.91SGVSYY2187 pKa = 11.51DD2188 pKa = 3.6MASNTLTLTGTGINSMLEE2206 pKa = 4.07SGEE2209 pKa = 4.91DD2210 pKa = 3.15ATTDD2214 pKa = 3.08IKK2216 pKa = 11.45DD2217 pKa = 3.48RR2218 pKa = 11.84LDD2220 pKa = 3.07WSKK2223 pKa = 11.19LVWTFNNNTSYY2234 pKa = 11.61DD2235 pKa = 3.78FGSGDD2240 pKa = 3.78LGNNTGDD2247 pKa = 5.15DD2248 pKa = 3.74ILSVKK2253 pKa = 10.3AVSDD2257 pKa = 3.49TSLEE2261 pKa = 3.93ITFTDD2266 pKa = 4.53DD2267 pKa = 3.33AANACGSGLEE2277 pKa = 4.31SFFNGFDD2284 pKa = 3.46TSNPNYY2290 pKa = 10.74APFSDD2295 pKa = 3.65GHH2297 pKa = 6.42KK2298 pKa = 9.49LTVTAGFSRR2307 pKa = 11.84DD2308 pKa = 3.64SNGNAATGDD2317 pKa = 4.03GYY2319 pKa = 11.74VNPDD2323 pKa = 3.0TSIVVFDD2330 pKa = 5.32LIQGVSSSHH2339 pKa = 6.25SSRR2342 pKa = 11.84TFDD2345 pKa = 3.97ANTSYY2350 pKa = 10.76TIYY2353 pKa = 10.62IRR2355 pKa = 11.84MDD2357 pKa = 3.5SDD2359 pKa = 3.71SSTLSTAGSGPGTWGTWSSANNLGTDD2385 pKa = 3.62DD2386 pKa = 3.89KK2387 pKa = 11.64VIFVGSGSVIKK2398 pKa = 10.9NNSNPAQEE2406 pKa = 3.85VTFYY2410 pKa = 10.84YY2411 pKa = 10.55KK2412 pKa = 10.44SAGLLEE2418 pKa = 4.91LNYY2421 pKa = 10.39SLNSRR2426 pKa = 11.84TVQLYY2431 pKa = 9.05IYY2433 pKa = 9.7LGVGSIARR2441 pKa = 11.84NNYY2444 pKa = 9.63ASSEE2448 pKa = 4.17KK2449 pKa = 10.51LWDD2452 pKa = 3.77GNISVGDD2459 pKa = 4.03LRR2461 pKa = 11.84PTTYY2465 pKa = 9.72STGKK2469 pKa = 10.47FNDD2472 pKa = 3.93FVLTTMPNGILTSQGLVV2489 pKa = 3.12

Molecular weight:
255.77 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A5C2HI14|A0A5C2HI14_9GAMM GTP diphosphokinase OS=Ectothiorhodospiraceae bacterium BW-2 OX=947515 GN=D5085_05745 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNIKK11 pKa = 10.05RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 6.03GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 10.06SVGGRR28 pKa = 11.84KK29 pKa = 8.25VLKK32 pKa = 10.25ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.31GRR39 pKa = 11.84ARR41 pKa = 11.84LALL44 pKa = 3.93

Molecular weight:
5.06 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3445

0

3445

1182593

33

6638

343.3

38.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.061 ± 0.052

0.958 ± 0.016

5.127 ± 0.037

6.697 ± 0.045

3.474 ± 0.028

6.752 ± 0.052

2.495 ± 0.032

5.848 ± 0.033

3.549 ± 0.045

11.693 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.28 ± 0.024

3.408 ± 0.04

4.472 ± 0.03

5.81 ± 0.06

6.274 ± 0.062

6.321 ± 0.052

5.335 ± 0.082

6.107 ± 0.039

1.349 ± 0.018

2.993 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski