Bosea sp. Root483D1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Boseaceae; Bosea; unclassified Bosea

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6046 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q9I3U7|A0A0Q9I3U7_9BRAD Chromosome partitioning protein ParA OS=Bosea sp. Root483D1 OX=1736544 GN=ASE66_25110 PE=4 SV=1
MM1 pKa = 7.63LEE3 pKa = 3.97GKK5 pKa = 10.26AILVVEE11 pKa = 4.82DD12 pKa = 3.44EE13 pKa = 4.53TMIAMMLDD21 pKa = 3.81DD22 pKa = 5.75LLTDD26 pKa = 4.23LGCSVVGPAQNLAQAQGLVGTTTCDD51 pKa = 3.17AAIVDD56 pKa = 4.56LNLNGTMSHH65 pKa = 6.89PLIEE69 pKa = 4.41ALVEE73 pKa = 4.03KK74 pKa = 10.09GVPVIVATGYY84 pKa = 11.11GGDD87 pKa = 4.05ASDD90 pKa = 4.67LPSGCRR96 pKa = 11.84VLAKK100 pKa = 10.0PYY102 pKa = 9.25TIQHH106 pKa = 5.4VEE108 pKa = 3.89QVLRR112 pKa = 11.84ACLLDD117 pKa = 3.51

Molecular weight: 12.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q9J570|A0A0Q9J570_9BRAD Uncharacterized protein OS=Bosea sp. Root483D1 OX=1736544 GN=ASE66_04675 PE=4 SV=1
MM1 pKa = 7.43GFSLLKK7 pKa = 9.99TKK9 pKa = 10.25FAQEE13 pKa = 3.74VLGRR17 pKa = 11.84SLAFYY22 pKa = 10.42LRR24 pKa = 11.84LVRR27 pKa = 11.84RR28 pKa = 11.84TNRR31 pKa = 11.84FVVEE35 pKa = 3.8PADD38 pKa = 3.48VYY40 pKa = 9.67EE41 pKa = 4.38HH42 pKa = 6.41VRR44 pKa = 11.84GEE46 pKa = 4.22LPLIIAMWHH55 pKa = 5.87GQHH58 pKa = 6.93IMIPFARR65 pKa = 11.84PEE67 pKa = 3.96WMPSCSLVSRR77 pKa = 11.84HH78 pKa = 6.0GDD80 pKa = 3.04GGFNAVALRR89 pKa = 11.84EE90 pKa = 4.09LGIGAIRR97 pKa = 11.84GSGASGKK104 pKa = 9.72KK105 pKa = 9.33VRR107 pKa = 11.84EE108 pKa = 4.11KK109 pKa = 11.01GGAPAFLAMVRR120 pKa = 11.84RR121 pKa = 11.84LAAGDD126 pKa = 3.97TMVLTADD133 pKa = 3.03IPKK136 pKa = 9.82RR137 pKa = 11.84ARR139 pKa = 11.84VCGPGIVQLARR150 pKa = 11.84ASGRR154 pKa = 11.84PIHH157 pKa = 6.79PIAVVTSRR165 pKa = 11.84RR166 pKa = 11.84IDD168 pKa = 3.49FNSWDD173 pKa = 3.8RR174 pKa = 11.84ASIGLPFGRR183 pKa = 11.84GAIVVGDD190 pKa = 4.02PVRR193 pKa = 11.84VARR196 pKa = 11.84DD197 pKa = 3.17ADD199 pKa = 3.77EE200 pKa = 4.63ATCEE204 pKa = 3.88AARR207 pKa = 11.84LAVQAGLDD215 pKa = 3.72AVHH218 pKa = 6.32EE219 pKa = 4.09RR220 pKa = 11.84AYY222 pKa = 10.94ALIGARR228 pKa = 11.84DD229 pKa = 3.63PGAGLRR235 pKa = 11.84EE236 pKa = 3.94AQAAKK241 pKa = 10.12IAAKK245 pKa = 10.46ASAAA249 pKa = 3.59

Molecular weight: 26.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6046

0

6046

1907448

41

5845

315.5

34.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.446 ± 0.041

0.801 ± 0.01

5.235 ± 0.028

5.584 ± 0.033

3.724 ± 0.02

8.912 ± 0.036

1.884 ± 0.017

5.111 ± 0.02

3.188 ± 0.033

10.646 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.309 ± 0.016

2.343 ± 0.02

5.341 ± 0.024

3.105 ± 0.019

7.225 ± 0.028

5.276 ± 0.026

5.119 ± 0.031

7.361 ± 0.027

1.315 ± 0.012

2.073 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski