endosymbiont of Escarpia spicata

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; sulfur-oxidizing symbionts

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3499 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A370DQB1|A0A370DQB1_9GAMM TPR_REGION domain-containing protein OS=endosymbiont of Escarpia spicata OX=2200908 GN=DIZ78_05625 PE=4 SV=1
MM1 pKa = 7.34LVWQHH6 pKa = 6.57AEE8 pKa = 3.98SEE10 pKa = 4.33DD11 pKa = 4.11GISCVGISRR20 pKa = 11.84EE21 pKa = 4.12TGEE24 pKa = 5.37DD25 pKa = 3.22PMKK28 pKa = 10.94AIILALALVGVLFAPSTALAEE49 pKa = 4.13DD50 pKa = 4.02TPLDD54 pKa = 3.52KK55 pKa = 10.84TVWTTSEE62 pKa = 4.33STWWWSLWPEE72 pKa = 4.03PVSCDD77 pKa = 2.84GQAFHH82 pKa = 7.35LIDD85 pKa = 3.65GVYY88 pKa = 9.28TYY90 pKa = 10.66SYY92 pKa = 10.03PADD95 pKa = 3.51PCGRR99 pKa = 11.84GRR101 pKa = 11.84SFFSPANALDD111 pKa = 3.74NHH113 pKa = 5.84GQWFQIDD120 pKa = 3.97FGVSLTLTRR129 pKa = 11.84IRR131 pKa = 11.84FYY133 pKa = 11.18QFVEE137 pKa = 4.11NTLMSSLPGTDD148 pKa = 2.51KK149 pKa = 10.62YY150 pKa = 11.55SRR152 pKa = 11.84YY153 pKa = 8.45INEE156 pKa = 3.71MRR158 pKa = 11.84FTFSDD163 pKa = 3.53GTEE166 pKa = 3.96EE167 pKa = 4.99LVAFPPGTPFSTRR180 pKa = 11.84EE181 pKa = 3.87SVTYY185 pKa = 10.95ADD187 pKa = 4.64FAAPHH192 pKa = 4.77TTEE195 pKa = 4.72SIRR198 pKa = 11.84ATIVSFHH205 pKa = 6.71NDD207 pKa = 2.75YY208 pKa = 11.51SLAGLYY214 pKa = 10.48ALEE217 pKa = 4.76IDD219 pKa = 4.44FFGAAGDD226 pKa = 4.34PDD228 pKa = 5.29DD229 pKa = 5.96LDD231 pKa = 5.58GDD233 pKa = 4.69GIVNADD239 pKa = 4.1DD240 pKa = 4.14NCPLSANPDD249 pKa = 3.85QLDD252 pKa = 3.56MDD254 pKa = 4.67HH255 pKa = 7.4DD256 pKa = 5.51GIGNICDD263 pKa = 4.06DD264 pKa = 5.67DD265 pKa = 4.65IDD267 pKa = 4.75GDD269 pKa = 3.89EE270 pKa = 4.65FANVDD275 pKa = 4.15DD276 pKa = 4.93NCPEE280 pKa = 4.17AANSLQSDD288 pKa = 3.91GDD290 pKa = 3.78LDD292 pKa = 5.11GIGDD296 pKa = 4.06ACDD299 pKa = 3.87PEE301 pKa = 4.85CNPRR305 pKa = 11.84LTVKK309 pKa = 10.7DD310 pKa = 3.53GWIGNQSSWWLQPWRR325 pKa = 11.84NTCDD329 pKa = 3.24GKK331 pKa = 10.97AQNAIDD337 pKa = 3.5GNYY340 pKa = 9.49RR341 pKa = 11.84FTYY344 pKa = 9.38SNYY347 pKa = 8.11WACNGDD353 pKa = 3.84LSPRR357 pKa = 11.84SFFAPRR363 pKa = 11.84FTGTDD368 pKa = 2.81VGTWMQVEE376 pKa = 4.68FPEE379 pKa = 4.7VYY381 pKa = 9.51TINGITLAQIKK392 pKa = 9.67EE393 pKa = 3.94RR394 pKa = 11.84TYY396 pKa = 10.5VSGLVRR402 pKa = 11.84PHH404 pKa = 7.29KK405 pKa = 10.61YY406 pKa = 9.89GAYY409 pKa = 10.22LKK411 pKa = 10.61DD412 pKa = 3.41AMVEE416 pKa = 4.13FSDD419 pKa = 4.29GSSVEE424 pKa = 3.98VTFPQQLEE432 pKa = 4.03ASVTFPEE439 pKa = 4.44IATQSVHH446 pKa = 3.36ITARR450 pKa = 11.84SFHH453 pKa = 6.2PTVTTNPAWLVVEE466 pKa = 4.78ADD468 pKa = 3.66FAGAPGDD475 pKa = 3.3VDD477 pKa = 5.64LNLEE481 pKa = 4.37VCSEE485 pKa = 4.16PAPLPPDD492 pKa = 3.46TDD494 pKa = 3.8GDD496 pKa = 4.35GVPDD500 pKa = 4.48HH501 pKa = 7.32LDD503 pKa = 3.52AFPNDD508 pKa = 3.46PTEE511 pKa = 3.97WSDD514 pKa = 3.74VDD516 pKa = 3.8GDD518 pKa = 4.68GIGDD522 pKa = 3.79NADD525 pKa = 4.0PDD527 pKa = 4.52DD528 pKa = 5.83DD529 pKa = 5.09NDD531 pKa = 5.78GIPDD535 pKa = 4.72DD536 pKa = 5.0EE537 pKa = 4.66DD538 pKa = 4.22TNPTEE543 pKa = 4.8ADD545 pKa = 3.36ALEE548 pKa = 5.2DD549 pKa = 3.75SDD551 pKa = 5.98GDD553 pKa = 4.3GIPNQDD559 pKa = 5.65DD560 pKa = 4.07PDD562 pKa = 5.41DD563 pKa = 5.9DD564 pKa = 4.48NDD566 pKa = 4.04GVLDD570 pKa = 4.18EE571 pKa = 6.33DD572 pKa = 4.91DD573 pKa = 5.57AFSKK577 pKa = 11.1DD578 pKa = 3.3PTEE581 pKa = 4.14WADD584 pKa = 3.3TDD586 pKa = 3.88GDD588 pKa = 4.48GVGDD592 pKa = 4.13NSDD595 pKa = 3.53NCITGYY601 pKa = 10.95NPGQADD607 pKa = 3.94ADD609 pKa = 3.75NDD611 pKa = 5.09GIGDD615 pKa = 3.78PCDD618 pKa = 3.85RR619 pKa = 11.84PIANAGMDD627 pKa = 3.65QSAHH631 pKa = 6.86PGTAVTLDD639 pKa = 3.78GTGSTDD645 pKa = 3.34PDD647 pKa = 3.31EE648 pKa = 5.14EE649 pKa = 4.6YY650 pKa = 10.43PLLFAWQLISAPPGSLASVQNPTSATPSLTPDD682 pKa = 2.89MMGDD686 pKa = 3.63YY687 pKa = 10.66LLEE690 pKa = 4.33LSVTDD695 pKa = 3.79QQGHH699 pKa = 5.26VSDD702 pKa = 4.12SDD704 pKa = 3.34KK705 pKa = 11.73VMVSTVNTAPTADD718 pKa = 3.75AGEE721 pKa = 4.68DD722 pKa = 3.47LAITQTGQWVALSGLASYY740 pKa = 11.15DD741 pKa = 3.65LDD743 pKa = 5.86GDD745 pKa = 4.99DD746 pKa = 5.63LIAYY750 pKa = 9.0QWMLVSKK757 pKa = 10.14PDD759 pKa = 3.38GSLAEE764 pKa = 4.31PAPADD769 pKa = 3.61TVEE772 pKa = 4.15TLFLADD778 pKa = 4.82LYY780 pKa = 11.12GSYY783 pKa = 10.7VFEE786 pKa = 4.34LTVTDD791 pKa = 4.28AYY793 pKa = 11.38YY794 pKa = 10.78SVSEE798 pKa = 4.12PDD800 pKa = 3.42QITVSLANVRR810 pKa = 11.84PVADD814 pKa = 4.18AGDD817 pKa = 3.82NLSVYY822 pKa = 10.27VGEE825 pKa = 4.42SVSLGGTGSHH835 pKa = 7.52DD836 pKa = 4.04ANGDD840 pKa = 3.53PLTYY844 pKa = 9.9HH845 pKa = 7.02WSLVLKK851 pKa = 10.17PDD853 pKa = 3.32GSTAEE858 pKa = 4.36LAAPDD863 pKa = 3.55QAMTGFMADD872 pKa = 2.99LAGTYY877 pKa = 9.79LVSLVVNDD885 pKa = 4.44GLQDD889 pKa = 3.82SEE891 pKa = 4.49PSSVTVTALTRR902 pKa = 11.84QDD904 pKa = 3.49EE905 pKa = 4.76VVTLVQDD912 pKa = 3.37VMDD915 pKa = 5.99RR916 pKa = 11.84INGFDD921 pKa = 3.25SSVFKK926 pKa = 11.05NKK928 pKa = 9.79NLAKK932 pKa = 10.55PFTNKK937 pKa = 9.72LNAALSKK944 pKa = 10.73LDD946 pKa = 3.63NGDD949 pKa = 3.46YY950 pKa = 11.35ADD952 pKa = 4.64ALNKK956 pKa = 10.2LEE958 pKa = 5.28HH959 pKa = 7.47DD960 pKa = 4.29ILPKK964 pKa = 9.84IDD966 pKa = 3.42GCATSGQPDD975 pKa = 3.58KK976 pKa = 11.25QDD978 pKa = 2.74WLTTCEE984 pKa = 4.17AQAEE988 pKa = 4.66VYY990 pKa = 10.42PLVQQAIEE998 pKa = 4.21LLRR1001 pKa = 11.84SLII1004 pKa = 4.0

Molecular weight: 108.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A370DR43|A0A370DR43_9GAMM YceI family protein OS=endosymbiont of Escarpia spicata OX=2200908 GN=DIZ78_06040 PE=4 SV=1
MM1 pKa = 7.6AKK3 pKa = 10.21KK4 pKa = 8.94KK5 pKa = 7.63TAKK8 pKa = 10.23KK9 pKa = 8.3KK10 pKa = 7.97TAVKK14 pKa = 10.21RR15 pKa = 11.84KK16 pKa = 8.79VSTTKK21 pKa = 10.37RR22 pKa = 11.84VAAKK26 pKa = 10.13KK27 pKa = 9.91KK28 pKa = 9.71ASTRR32 pKa = 11.84KK33 pKa = 9.74KK34 pKa = 10.4SVAKK38 pKa = 10.45KK39 pKa = 9.92SASKK43 pKa = 10.51KK44 pKa = 9.65KK45 pKa = 10.54AVTKK49 pKa = 10.55KK50 pKa = 9.21KK51 pKa = 8.18TVKK54 pKa = 10.09KK55 pKa = 9.86RR56 pKa = 11.84AAKK59 pKa = 10.18KK60 pKa = 10.03KK61 pKa = 9.24VAAKK65 pKa = 10.13KK66 pKa = 8.99KK67 pKa = 7.06TVKK70 pKa = 10.12KK71 pKa = 9.95RR72 pKa = 11.84AAKK75 pKa = 10.18KK76 pKa = 10.03KK77 pKa = 9.24VAAKK81 pKa = 10.13KK82 pKa = 8.99KK83 pKa = 7.06TVKK86 pKa = 10.12KK87 pKa = 9.95RR88 pKa = 11.84AAKK91 pKa = 10.18KK92 pKa = 10.03KK93 pKa = 9.24VAAKK97 pKa = 10.13KK98 pKa = 8.99KK99 pKa = 7.06TVKK102 pKa = 10.12KK103 pKa = 9.95RR104 pKa = 11.84AAKK107 pKa = 10.18KK108 pKa = 10.03KK109 pKa = 9.24VAAKK113 pKa = 10.13KK114 pKa = 8.99KK115 pKa = 7.06TVKK118 pKa = 10.12KK119 pKa = 9.95RR120 pKa = 11.84AAKK123 pKa = 10.18KK124 pKa = 10.03KK125 pKa = 9.24VAAKK129 pKa = 10.13KK130 pKa = 8.99KK131 pKa = 7.06TVKK134 pKa = 10.12KK135 pKa = 9.95RR136 pKa = 11.84AAKK139 pKa = 10.18KK140 pKa = 10.03KK141 pKa = 9.24VAAKK145 pKa = 10.13KK146 pKa = 8.99KK147 pKa = 7.06TVKK150 pKa = 10.08KK151 pKa = 9.89RR152 pKa = 11.84AARR155 pKa = 11.84PRR157 pKa = 11.84KK158 pKa = 7.53VTPRR162 pKa = 11.84KK163 pKa = 9.84KK164 pKa = 9.64ATQKK168 pKa = 11.07

Molecular weight: 18.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3499

0

3499

1169978

17

4119

334.4

37.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.017 ± 0.04

0.979 ± 0.017

5.918 ± 0.035

6.687 ± 0.042

3.783 ± 0.028

7.694 ± 0.044

2.264 ± 0.022

5.746 ± 0.035

4.284 ± 0.041

10.816 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.522 ± 0.024

3.448 ± 0.029

4.537 ± 0.03

4.098 ± 0.034

6.164 ± 0.047

6.124 ± 0.032

5.21 ± 0.044

6.787 ± 0.04

1.285 ± 0.016

2.635 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski