Microbacterium phage McGalleon

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ilzatvirus; Microbacterium virus McGalleon

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A516KQY9|A0A516KQY9_9CAUD Uncharacterized protein OS=Microbacterium phage McGalleon OX=2590936 GN=3 PE=4 SV=1
MM1 pKa = 7.66DD2 pKa = 4.43TNAHH6 pKa = 6.46GLPKK10 pKa = 9.29PTFGPLVPFMEE21 pKa = 4.76EE22 pKa = 3.65FTTPEE27 pKa = 3.84WIEE30 pKa = 3.52IEE32 pKa = 4.12GRR34 pKa = 11.84RR35 pKa = 11.84FPWDD39 pKa = 3.63PEE41 pKa = 3.74QEE43 pKa = 4.57TYY45 pKa = 10.77VGLYY49 pKa = 9.23VEE51 pKa = 5.7PYY53 pKa = 9.08LTDD56 pKa = 3.31GTLTEE61 pKa = 4.97DD62 pKa = 2.9IYY64 pKa = 11.83YY65 pKa = 10.22EE66 pKa = 4.05IEE68 pKa = 3.96EE69 pKa = 4.48GG70 pKa = 3.87

Molecular weight: 8.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A516KQZ3|A0A516KQZ3_9CAUD Uncharacterized protein OS=Microbacterium phage McGalleon OX=2590936 GN=61 PE=4 SV=1
MM1 pKa = 7.14FVLARR6 pKa = 11.84TGKK9 pKa = 10.01GRR11 pKa = 11.84PTLQHH16 pKa = 6.57GLAEE20 pKa = 4.95DD21 pKa = 4.13GQRR24 pKa = 11.84TVCGLDD30 pKa = 3.05VTVWSRR36 pKa = 11.84AYY38 pKa = 9.56QPKK41 pKa = 10.41AIPEE45 pKa = 3.98ILCRR49 pKa = 11.84KK50 pKa = 7.8CAKK53 pKa = 10.03RR54 pKa = 11.84LRR56 pKa = 11.84GEE58 pKa = 3.87AA59 pKa = 3.51

Molecular weight: 6.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13326

35

807

211.5

23.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.868 ± 0.444

0.615 ± 0.096

6.521 ± 0.273

6.476 ± 0.481

3.092 ± 0.19

7.842 ± 0.344

1.786 ± 0.175

5.23 ± 0.388

4.758 ± 0.365

8.54 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.214 ± 0.13

3.339 ± 0.157

4.87 ± 0.269

4.06 ± 0.214

5.786 ± 0.365

5.928 ± 0.287

6.446 ± 0.263

7.474 ± 0.257

2.109 ± 0.236

3.047 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski