Prevotella multisaccharivorax DSM 17128

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; Prevotella multisaccharivorax

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2694 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8NBJ6|F8NBJ6_9BACT Ribosomal silencing factor RsfS OS=Prevotella multisaccharivorax DSM 17128 OX=688246 GN=rsfS PE=3 SV=1
MM1 pKa = 7.62KK2 pKa = 10.58YY3 pKa = 10.46EE4 pKa = 4.38EE5 pKa = 4.55IEE7 pKa = 3.98KK8 pKa = 10.53KK9 pKa = 9.96PYY11 pKa = 10.35HH12 pKa = 5.62FTLEE16 pKa = 4.41RR17 pKa = 11.84IDD19 pKa = 4.76PYY21 pKa = 11.47DD22 pKa = 3.71EE23 pKa = 4.43NVPEE27 pKa = 4.26AVIPEE32 pKa = 4.18DD33 pKa = 3.12KK34 pKa = 10.62CLYY37 pKa = 8.31FQPEE41 pKa = 4.09EE42 pKa = 3.94LLEE45 pKa = 3.88LRR47 pKa = 11.84NFYY50 pKa = 10.45KK51 pKa = 10.55RR52 pKa = 11.84EE53 pKa = 3.77GRR55 pKa = 11.84DD56 pKa = 3.32APFEE60 pKa = 3.93FLQDD64 pKa = 3.95YY65 pKa = 11.08APDD68 pKa = 4.15SYY70 pKa = 12.07DD71 pKa = 3.68EE72 pKa = 4.9DD73 pKa = 3.14WWALLDD79 pKa = 3.82YY80 pKa = 10.49LYY82 pKa = 10.94SCTSIDD88 pKa = 3.69MIVAEE93 pKa = 4.61TAAAPDD99 pKa = 3.72PVINRR104 pKa = 11.84DD105 pKa = 3.39ALVARR110 pKa = 11.84LLNEE114 pKa = 4.41LPYY117 pKa = 11.19SLDD120 pKa = 3.83DD121 pKa = 3.93YY122 pKa = 11.65NIIAPQEE129 pKa = 4.48LIDD132 pKa = 6.33DD133 pKa = 4.09VLDD136 pKa = 4.82DD137 pKa = 5.72NFTDD141 pKa = 3.35WVGIRR146 pKa = 11.84IRR148 pKa = 11.84IEE150 pKa = 3.8LDD152 pKa = 2.99NALVDD157 pKa = 3.53ADD159 pKa = 5.36FYY161 pKa = 11.76DD162 pKa = 4.4SEE164 pKa = 4.71CKK166 pKa = 10.35

Molecular weight: 19.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8N5Q2|F8N5Q2_9BACT Transcriptional regulator AraC family OS=Prevotella multisaccharivorax DSM 17128 OX=688246 GN=Premu_0588 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.81RR3 pKa = 11.84GVLACLLVRR12 pKa = 11.84RR13 pKa = 11.84CRR15 pKa = 11.84LSHH18 pKa = 6.64HH19 pKa = 6.95GILVEE24 pKa = 4.33RR25 pKa = 11.84GSDD28 pKa = 3.2GTVFGKK34 pKa = 10.64EE35 pKa = 4.11FVHH38 pKa = 6.64HH39 pKa = 6.67GWHH42 pKa = 5.63FHH44 pKa = 6.78IIPGGGEE51 pKa = 3.83ANVAVSLANYY61 pKa = 8.1GHH63 pKa = 6.49EE64 pKa = 3.93AVFVSKK70 pKa = 10.68LPKK73 pKa = 10.08HH74 pKa = 6.82EE75 pKa = 3.99IRR77 pKa = 11.84QITVNALRR85 pKa = 11.84RR86 pKa = 11.84YY87 pKa = 9.8GVDD90 pKa = 2.16TRR92 pKa = 11.84YY93 pKa = 9.73IVRR96 pKa = 11.84GGATVSGRR104 pKa = 11.84SQCLRR109 pKa = 11.84VAEE112 pKa = 4.0AMRR115 pKa = 11.84LHH117 pKa = 6.92RR118 pKa = 5.48

Molecular weight: 13.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2694

0

2694

975933

20

2513

362.3

40.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.291 ± 0.045

1.283 ± 0.018

6.03 ± 0.036

5.638 ± 0.047

4.348 ± 0.028

6.926 ± 0.04

2.255 ± 0.022

6.389 ± 0.041

6.408 ± 0.04

8.847 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.823 ± 0.021

5.055 ± 0.039

3.701 ± 0.022

3.612 ± 0.022

5.106 ± 0.038

6.266 ± 0.036

5.81 ± 0.037

6.533 ± 0.032

1.317 ± 0.017

4.361 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski