Microbacterium phage Fireman

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Metamorphoovirus; Microbacterium virus Fireman

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481VVW6|A0A481VVW6_9CAUD Uncharacterized protein OS=Microbacterium phage Fireman OX=2530118 GN=41 PE=4 SV=1
MM1 pKa = 7.89AEE3 pKa = 3.94PTLTDD8 pKa = 4.0DD9 pKa = 5.11AAATPAAAFVVPTTPPPTQACGWPVVYY36 pKa = 10.43LDD38 pKa = 4.6CAEE41 pKa = 4.68GDD43 pKa = 3.78CDD45 pKa = 6.56AYY47 pKa = 10.89DD48 pKa = 3.46QWPEE52 pKa = 3.93DD53 pKa = 3.25QRR55 pKa = 11.84AAARR59 pKa = 11.84AWFEE63 pKa = 3.75AQAIDD68 pKa = 4.23ILWNASGGVFGVCDD82 pKa = 3.58VEE84 pKa = 5.72LRR86 pKa = 11.84PCRR89 pKa = 11.84QGCDD93 pKa = 3.87GNSDD97 pKa = 3.1WATTFWGRR105 pKa = 11.84GPGFSPGFPRR115 pKa = 11.84VGGGPAGSSFYY126 pKa = 10.22PVLVSGQWFNITCGCLGQCRR146 pKa = 11.84CSPSGPNVISLPDD159 pKa = 4.64PIVEE163 pKa = 4.25VTEE166 pKa = 3.99VTIDD170 pKa = 4.46GITVDD175 pKa = 3.59PATYY179 pKa = 10.16RR180 pKa = 11.84IDD182 pKa = 3.31RR183 pKa = 11.84GRR185 pKa = 11.84WLIRR189 pKa = 11.84DD190 pKa = 3.38GDD192 pKa = 4.08VWPGCQDD199 pKa = 3.2MNVAPDD205 pKa = 3.55AVGSFVVRR213 pKa = 11.84YY214 pKa = 9.41KK215 pKa = 10.79RR216 pKa = 11.84GVQVPAGGQIAAGRR230 pKa = 11.84LACEE234 pKa = 4.43LAMAACGDD242 pKa = 4.45DD243 pKa = 5.29DD244 pKa = 5.82CALPDD249 pKa = 3.18NWQTITRR256 pKa = 11.84QGLTVNADD264 pKa = 3.34PNMDD268 pKa = 3.55GTQVTGIWSIDD279 pKa = 3.07EE280 pKa = 4.27WIKK283 pKa = 10.36QVNRR287 pKa = 11.84PRR289 pKa = 11.84TFATVRR295 pKa = 11.84SVDD298 pKa = 3.88LPNLRR303 pKa = 4.34

Molecular weight: 32.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8VYU1|A0A4P8VYU1_9CAUD Uncharacterized protein OS=Microbacterium phage Fireman OX=2530118 GN=97 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84RR3 pKa = 11.84HH4 pKa = 6.08PGGRR8 pKa = 11.84PPLPARR14 pKa = 11.84MLWGTPLGNGKK25 pKa = 9.47AAGEE29 pKa = 4.12RR30 pKa = 11.84LGVAARR36 pKa = 11.84TGVSGPHH43 pKa = 4.52SHH45 pKa = 6.72HH46 pKa = 6.02RR47 pKa = 11.84TSNRR51 pKa = 11.84IAVRR55 pKa = 11.84TSS57 pKa = 2.58

Molecular weight: 6.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

17444

29

1202

181.7

19.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.125 ± 0.443

0.814 ± 0.135

6.57 ± 0.326

6.14 ± 0.389

2.499 ± 0.137

8.725 ± 0.255

2.012 ± 0.169

4.546 ± 0.216

2.614 ± 0.195

7.779 ± 0.4

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.454 ± 0.12

2.626 ± 0.126

5.847 ± 0.283

3.136 ± 0.182

7.086 ± 0.35

5.693 ± 0.285

7.166 ± 0.345

7.722 ± 0.2

2.018 ± 0.168

2.431 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski