Infectious spleen and kidney necrosis virus (isolate Mandarin fish/China/Nanhai/1998) (ISKNV)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Iridoviridae; Alphairidovirinae; Megalocytivirus; Infectious spleen and kidney necrosis virus

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 125 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8QUU5|Q8QUU5_ISKNN ORF015R OS=Infectious spleen and kidney necrosis virus (isolate Mandarin fish/China/Nanhai/1998) OX=654923 PE=4 SV=1
MM1 pKa = 7.9SSEE4 pKa = 3.94LTLWRR9 pKa = 11.84SVAAACGATYY19 pKa = 10.21PLVRR23 pKa = 11.84SYY25 pKa = 11.16VAALEE30 pKa = 4.43SGCDD34 pKa = 2.93SGTTFRR40 pKa = 11.84NYY42 pKa = 8.2WSMRR46 pKa = 11.84SAHH49 pKa = 6.23IMAGNPFEE57 pKa = 5.94DD58 pKa = 4.04DD59 pKa = 4.89DD60 pKa = 4.54YY61 pKa = 11.98FEE63 pKa = 4.45TTSGVVISMAMVAEE77 pKa = 4.81HH78 pKa = 7.2CDD80 pKa = 3.65SLPDD84 pKa = 4.36LLAAYY89 pKa = 8.99NAAAQTAPLFDD100 pKa = 4.44CPMLQGFLDD109 pKa = 3.89TVMQSQLLQDD119 pKa = 3.37IHH121 pKa = 7.11TDD123 pKa = 3.26VAASGNSANLLNIISQNIGKK143 pKa = 9.22HH144 pKa = 6.27DD145 pKa = 4.06DD146 pKa = 4.08FIKK149 pKa = 10.71QFSGDD154 pKa = 3.2IIGYY158 pKa = 9.46LEE160 pKa = 4.24TMPVDD165 pKa = 3.74QLAQMMEE172 pKa = 4.58VIKK175 pKa = 10.77EE176 pKa = 3.96MTGQGGDD183 pKa = 3.79EE184 pKa = 4.86LIQAIMAHH192 pKa = 5.53ATVV195 pKa = 3.7

Molecular weight: 21.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8QUU3|Q8QUU3_ISKNN ORF017L OS=Infectious spleen and kidney necrosis virus (isolate Mandarin fish/China/Nanhai/1998) OX=654923 PE=4 SV=1
MM1 pKa = 7.21PQPIIFKK8 pKa = 9.06CTASPSALRR17 pKa = 11.84RR18 pKa = 11.84YY19 pKa = 6.56TASPRR24 pKa = 11.84KK25 pKa = 9.39RR26 pKa = 11.84RR27 pKa = 11.84SPTKK31 pKa = 9.66RR32 pKa = 11.84RR33 pKa = 11.84SPAKK37 pKa = 9.54RR38 pKa = 11.84RR39 pKa = 11.84SPAKK43 pKa = 9.58RR44 pKa = 11.84RR45 pKa = 11.84SPAKK49 pKa = 9.19RR50 pKa = 11.84RR51 pKa = 11.84SPVRR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84SPARR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 3.46

Molecular weight: 7.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

125

0

125

34670

40

1208

277.4

30.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.89 ± 0.258

3.173 ± 0.201

5.913 ± 0.187

3.813 ± 0.188

2.982 ± 0.134

5.645 ± 0.19

3.761 ± 0.174

4.327 ± 0.117

3.277 ± 0.177

8.22 ± 0.218

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.029 ± 0.116

3.556 ± 0.135

5.379 ± 0.214

3.865 ± 0.15

6.507 ± 0.265

5.887 ± 0.194

7.318 ± 0.253

8.474 ± 0.25

1.177 ± 0.068

3.807 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski