Pseudomonas phage PspYZU05

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; unclassified Tevenvirinae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 224 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U7N2D5|A0A2U7N2D5_9CAUD Uncharacterized protein OS=Pseudomonas phage PspYZU05 OX=1983556 GN=PspYZU05_38 PE=4 SV=1
MM1 pKa = 7.59AYY3 pKa = 9.41EE4 pKa = 3.89VSEE7 pKa = 4.37NKK9 pKa = 9.84VEE11 pKa = 4.31VTQSLLLDD19 pKa = 4.31RR20 pKa = 11.84AQEE23 pKa = 3.9LCVMFKK29 pKa = 10.28EE30 pKa = 4.22IKK32 pKa = 10.27EE33 pKa = 4.06MAKK36 pKa = 10.13EE37 pKa = 4.11LEE39 pKa = 4.48LDD41 pKa = 3.74TEE43 pKa = 4.65FNQYY47 pKa = 9.79EE48 pKa = 4.37GSFEE52 pKa = 3.9FRR54 pKa = 11.84DD55 pKa = 3.93WNSSSCYY62 pKa = 10.57GEE64 pKa = 5.34DD65 pKa = 3.45EE66 pKa = 4.46ILRR69 pKa = 11.84ADD71 pKa = 4.43ADD73 pKa = 4.17SSWVQSSCC81 pKa = 4.48

Molecular weight: 9.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U7NBU5|A0A2U7NBU5_9CAUD Uncharacterized protein OS=Pseudomonas phage PspYZU05 OX=1983556 GN=PspYZU05_127 PE=4 SV=1
MM1 pKa = 7.21TLALFVSGVVCFLLILVYY19 pKa = 9.18MLPAIVAGIVRR30 pKa = 11.84HH31 pKa = 5.88NNFAAITVLNVLLGWSLLGWVVALVWAFTNNRR63 pKa = 11.84QPIQGKK69 pKa = 6.95MM70 pKa = 3.15

Molecular weight: 7.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

224

0

224

53388

37

1260

238.3

27.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.975 ± 0.167

1.135 ± 0.078

6.164 ± 0.122

6.955 ± 0.172

4.243 ± 0.11

5.632 ± 0.201

1.903 ± 0.082

7.451 ± 0.119

7.567 ± 0.208

8.461 ± 0.158

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.574 ± 0.084

5.752 ± 0.119

3.388 ± 0.089

3.218 ± 0.105

4.387 ± 0.113

7.245 ± 0.143

5.859 ± 0.196

6.571 ± 0.135

1.144 ± 0.067

4.376 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski