Bacillus sp. B1-b2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4701 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I1EYX1|A0A6I1EYX1_9BACI Serine/threonine protein kinase OS=Bacillus sp. B1-b2 OX=2653201 GN=F9279_22295 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.23KK3 pKa = 10.04KK4 pKa = 10.51FSFISGLVAFTLLFAPITNHH24 pKa = 5.58TYY26 pKa = 10.06AAEE29 pKa = 4.13SSSVEE34 pKa = 3.9EE35 pKa = 4.47LEE37 pKa = 4.37EE38 pKa = 4.6ISYY41 pKa = 11.05EE42 pKa = 4.09DD43 pKa = 3.0AGVRR47 pKa = 11.84EE48 pKa = 4.28DD49 pKa = 3.94VYY51 pKa = 11.7NVTEE55 pKa = 4.24DD56 pKa = 3.82VYY58 pKa = 11.56GVTDD62 pKa = 3.72DD63 pKa = 4.08VYY65 pKa = 11.55GVTEE69 pKa = 4.02DD70 pKa = 3.97VYY72 pKa = 11.71NVTEE76 pKa = 4.21DD77 pKa = 3.83VYY79 pKa = 11.56GVTEE83 pKa = 4.3DD84 pKa = 3.89VYY86 pKa = 11.55GVTDD90 pKa = 3.72DD91 pKa = 4.08VYY93 pKa = 11.55GVTEE97 pKa = 4.33DD98 pKa = 3.89VYY100 pKa = 11.55GVTDD104 pKa = 3.82DD105 pKa = 4.14VYY107 pKa = 11.53GVTDD111 pKa = 3.83DD112 pKa = 4.14VYY114 pKa = 11.53GVTDD118 pKa = 3.83DD119 pKa = 4.14VYY121 pKa = 11.53GVTDD125 pKa = 3.7DD126 pKa = 4.08VYY128 pKa = 11.55GVTEE132 pKa = 4.33DD133 pKa = 3.89VYY135 pKa = 11.55GVTDD139 pKa = 3.82DD140 pKa = 4.14VYY142 pKa = 11.53GVTDD146 pKa = 3.7DD147 pKa = 4.08VYY149 pKa = 11.55GVTEE153 pKa = 4.68DD154 pKa = 4.47VYY156 pKa = 11.63LSDD159 pKa = 3.78IAVNEE164 pKa = 4.04AGMIDD169 pKa = 4.72LDD171 pKa = 3.87YY172 pKa = 10.91STYY175 pKa = 10.81SQVGLNIDD183 pKa = 3.54VEE185 pKa = 4.46EE186 pKa = 4.38VKK188 pKa = 10.93DD189 pKa = 3.68IVKK192 pKa = 10.87SNAEE196 pKa = 3.69AKK198 pKa = 10.79LNINKK203 pKa = 9.92GDD205 pKa = 3.71VTVEE209 pKa = 3.95LPLALLNIDD218 pKa = 3.45QNVTIKK224 pKa = 10.49ISPRR228 pKa = 11.84QYY230 pKa = 10.45EE231 pKa = 4.15GSLGQIYY238 pKa = 10.3DD239 pKa = 3.47FSIQKK244 pKa = 10.36EE245 pKa = 4.08DD246 pKa = 2.95GTYY249 pKa = 10.0ISKK252 pKa = 10.87FEE254 pKa = 4.14QNPITLKK261 pKa = 10.72FVVDD265 pKa = 3.75STKK268 pKa = 9.43VTNWDD273 pKa = 3.37SVAVYY278 pKa = 10.61YY279 pKa = 10.14IDD281 pKa = 3.71EE282 pKa = 4.57NGVIAEE288 pKa = 4.56KK289 pKa = 10.7LSPSKK294 pKa = 10.84INATTGEE301 pKa = 4.01VWVEE305 pKa = 3.97VTHH308 pKa = 6.23FSSYY312 pKa = 11.09GVFEE316 pKa = 4.11EE317 pKa = 5.27AKK319 pKa = 10.82DD320 pKa = 3.81NSGDD324 pKa = 3.86NNGVTPGNTTPGNNGTTGNTSNNATNGTTGNTNSNATSGSTNTTVTNATPVASNTNATTTTSGNQLPNTATNNMNAILFGLTIVALGGVLLFVRR418 pKa = 11.84NRR420 pKa = 11.84RR421 pKa = 11.84AIKK424 pKa = 10.24NN425 pKa = 3.56

Molecular weight: 45.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I1F9K2|A0A6I1F9K2_9BACI Sporulation protein YtfJ OS=Bacillus sp. B1-b2 OX=2653201 GN=ytfJ PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.59RR3 pKa = 11.84TFQPNKK9 pKa = 8.24RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSANGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4701

0

4701

1366001

26

3527

290.6

32.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.12 ± 0.039

0.708 ± 0.01

4.955 ± 0.027

7.669 ± 0.044

4.586 ± 0.031

6.419 ± 0.04

1.996 ± 0.018

8.574 ± 0.039

7.326 ± 0.032

9.94 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.701 ± 0.019

4.985 ± 0.032

3.274 ± 0.021

3.647 ± 0.023

3.629 ± 0.026

6.453 ± 0.029

5.505 ± 0.031

6.685 ± 0.035

1.014 ± 0.014

3.814 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski