Eragrostis curvula streak virus (isolate Eragrostis curvula/South Africa/g382/2008)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Eragrovirus; Eragrostis curvula streak virus

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C3UV59|C3UV59_ECSVG Capsid protein OS=Eragrostis curvula streak virus (isolate Eragrostis curvula/South Africa/g382/2008) OX=1559364 PE=3 SV=1
MM1 pKa = 7.54SGPPPCDD8 pKa = 2.59RR9 pKa = 11.84TEE11 pKa = 4.05PVQTIPLLLCHH22 pKa = 6.47GRR24 pKa = 11.84RR25 pKa = 11.84SLPLCHH31 pKa = 7.0AEE33 pKa = 4.03VLPPQDD39 pKa = 3.76LGQIRR44 pKa = 11.84YY45 pKa = 8.53LVKK48 pKa = 10.22RR49 pKa = 11.84FKK51 pKa = 11.05ARR53 pKa = 11.84VLIRR57 pKa = 11.84TIAEE61 pKa = 4.12QYY63 pKa = 10.31SGSSLADD70 pKa = 3.27KK71 pKa = 11.31LEE73 pKa = 4.92LIWTFCDD80 pKa = 3.62TLQHH84 pKa = 5.73EE85 pKa = 4.93AEE87 pKa = 4.39EE88 pKa = 4.43EE89 pKa = 4.04

Molecular weight: 10.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C3UV60|C3UV60_ECSVG Replication-associated protein OS=Eragrostis curvula streak virus (isolate Eragrostis curvula/South Africa/g382/2008) OX=1559364 PE=3 SV=1
MM1 pKa = 7.25KK2 pKa = 10.2RR3 pKa = 11.84KK4 pKa = 9.98RR5 pKa = 11.84NEE7 pKa = 3.49AVPAGRR13 pKa = 11.84RR14 pKa = 11.84YY15 pKa = 7.56PQRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84MYY22 pKa = 8.94YY23 pKa = 9.74RR24 pKa = 11.84PRR26 pKa = 11.84KK27 pKa = 9.07PFFPRR32 pKa = 11.84PVYY35 pKa = 9.8TRR37 pKa = 11.84SSSVKK42 pKa = 10.13RR43 pKa = 11.84PALQISGLVYY53 pKa = 10.72GNSSTGAVKK62 pKa = 10.48INTGALSLVTAFKK75 pKa = 10.9AGTAEE80 pKa = 4.15EE81 pKa = 4.72CRR83 pKa = 11.84HH84 pKa = 5.51SNQTIVKK91 pKa = 9.19SFDD94 pKa = 3.02ISGTLYY100 pKa = 10.21VQSPTSSNCGPVVVYY115 pKa = 8.44FWLIYY120 pKa = 10.41DD121 pKa = 3.97SEE123 pKa = 4.23PRR125 pKa = 11.84QAIPNITDD133 pKa = 3.44VFSMPWTSVPSSWRR147 pKa = 11.84ISRR150 pKa = 11.84SSSHH154 pKa = 6.24RR155 pKa = 11.84FVVKK159 pKa = 10.41RR160 pKa = 11.84KK161 pKa = 5.9WHH163 pKa = 5.94YY164 pKa = 10.77EE165 pKa = 3.86LMSDD169 pKa = 4.97GVLPQSNTKK178 pKa = 9.56VQTHH182 pKa = 5.82NPVSRR187 pKa = 11.84NMMDD191 pKa = 3.68FSKK194 pKa = 10.94YY195 pKa = 9.92INNLGVPTEE204 pKa = 4.1WMSTGDD210 pKa = 3.64GTIGDD215 pKa = 3.98IKK217 pKa = 10.82KK218 pKa = 10.37GALYY222 pKa = 10.24LAAACRR228 pKa = 11.84QGIVGDD234 pKa = 3.71ATKK237 pKa = 9.22ITIEE241 pKa = 4.15VEE243 pKa = 4.59FIGQSRR249 pKa = 11.84TYY251 pKa = 9.41FKK253 pKa = 11.05SIGYY257 pKa = 7.76QQ258 pKa = 3.02

Molecular weight: 29.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

714

89

367

238.0

27.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.742 ± 0.156

1.961 ± 0.569

5.322 ± 1.094

5.462 ± 1.098

3.922 ± 0.394

6.022 ± 0.454

3.081 ± 0.678

4.902 ± 0.669

5.742 ± 0.536

7.283 ± 1.765

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.401 ± 0.579

3.782 ± 0.82

5.742 ± 0.844

4.482 ± 0.307

7.283 ± 0.455

9.524 ± 0.964

5.462 ± 0.704

7.143 ± 0.62

2.241 ± 0.332

3.501 ± 0.658
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski