Clostridiales bacterium CHKCI001

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; unclassified Eubacteriales

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3078 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A143XE68|A0A143XE68_9FIRM DNA topoisomerase OS=Clostridiales bacterium CHKCI001 OX=1780378 GN=topB_1 PE=3 SV=1
MM1 pKa = 7.28TPDD4 pKa = 2.89TGMTSDD10 pKa = 3.98MGADD14 pKa = 3.86FDD16 pKa = 4.39NEE18 pKa = 4.15TVARR22 pKa = 11.84ALAATIDD29 pKa = 3.65EE30 pKa = 4.76VYY32 pKa = 11.14NRR34 pKa = 11.84TGKK37 pKa = 10.2NSILVTHH44 pKa = 6.1SQGGGPGWTAAQYY57 pKa = 8.93TDD59 pKa = 4.56HH60 pKa = 7.1IAAIVAIEE68 pKa = 4.24PGGAPAADD76 pKa = 3.5SADD79 pKa = 3.65YY80 pKa = 10.88KK81 pKa = 11.31AVTQKK86 pKa = 10.88NIPVTFYY93 pKa = 10.83FGDD96 pKa = 4.15YY97 pKa = 9.85IDD99 pKa = 5.31NGDD102 pKa = 4.19PSIQATGMWQAMRR115 pKa = 11.84EE116 pKa = 4.11TCYY119 pKa = 11.16DD120 pKa = 3.09FAEE123 pKa = 5.16AYY125 pKa = 9.04TEE127 pKa = 4.03QEE129 pKa = 4.55GNCTVIDD136 pKa = 4.61LPAEE140 pKa = 4.82GITGNDD146 pKa = 2.55HH147 pKa = 7.69FMFQDD152 pKa = 3.81LNNDD156 pKa = 3.99VIADD160 pKa = 4.2HH161 pKa = 6.74IEE163 pKa = 3.42NWIQNNVKK171 pKa = 10.44

Molecular weight: 18.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A143XP74|A0A143XP74_9FIRM ABC-2 family transporter protein OS=Clostridiales bacterium CHKCI001 OX=1780378 GN=NDGK_01110 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.05VHH16 pKa = 5.81GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 4.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3078

0

3078

979425

29

3353

318.2

35.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.667 ± 0.046

1.505 ± 0.02

5.11 ± 0.034

7.627 ± 0.052

4.187 ± 0.035

6.698 ± 0.042

1.782 ± 0.021

8.315 ± 0.037

7.002 ± 0.041

8.998 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.107 ± 0.027

4.696 ± 0.03

3.179 ± 0.023

3.836 ± 0.032

4.072 ± 0.028

5.843 ± 0.038

5.433 ± 0.041

6.641 ± 0.036

1.049 ± 0.016

4.253 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski