Mycobacterium phage ThetaBob

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Thetabobvirus; Mycobacterium virus ThetaBob

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 106 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EMP0|A0A4Y6EMP0_9CAUD Uncharacterized protein OS=Mycobacterium phage ThetaBob OX=2588513 GN=81 PE=4 SV=1
MM1 pKa = 7.44TCLLCDD7 pKa = 3.96HH8 pKa = 6.77SRR10 pKa = 11.84SSHH13 pKa = 6.16APQCRR18 pKa = 11.84VRR20 pKa = 11.84MGVDD24 pKa = 3.13RR25 pKa = 11.84DD26 pKa = 3.78DD27 pKa = 3.68MNTYY31 pKa = 8.02TICLCPGFEE40 pKa = 4.07GTEE43 pKa = 4.14DD44 pKa = 3.92GEE46 pKa = 4.23EE47 pKa = 4.1DD48 pKa = 3.26

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EVJ2|A0A4Y6EVJ2_9CAUD Uncharacterized protein OS=Mycobacterium phage ThetaBob OX=2588513 GN=72 PE=4 SV=1
MM1 pKa = 7.33TGRR4 pKa = 11.84VLTPIEE10 pKa = 4.13VEE12 pKa = 4.43KK13 pKa = 10.5VAWWTQLGWTAAQIADD29 pKa = 3.91KK30 pKa = 10.73LGCTTRR36 pKa = 11.84TVSRR40 pKa = 11.84ARR42 pKa = 11.84AKK44 pKa = 10.82SGVAKK49 pKa = 9.61PKK51 pKa = 10.37PPALSAEE58 pKa = 4.39VLAEE62 pKa = 4.13AARR65 pKa = 11.84MLADD69 pKa = 3.78GASQNEE75 pKa = 4.29VARR78 pKa = 11.84TLGVGQSTISAHH90 pKa = 6.15FRR92 pKa = 11.84GQGWTRR98 pKa = 11.84EE99 pKa = 3.97QSIEE103 pKa = 3.86WVSFLRR109 pKa = 11.84RR110 pKa = 11.84YY111 pKa = 10.26RR112 pKa = 11.84GVAA115 pKa = 2.99

Molecular weight: 12.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

106

0

106

18243

30

1174

172.1

18.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.53 ± 0.388

1.179 ± 0.13

6.123 ± 0.221

6.019 ± 0.294

2.949 ± 0.137

8.683 ± 0.683

2.187 ± 0.183

4.276 ± 0.166

3.41 ± 0.201

7.778 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.209 ± 0.133

3.41 ± 0.154

6.013 ± 0.184

3.744 ± 0.171

7.016 ± 0.4

5.673 ± 0.207

6.474 ± 0.249

7.565 ± 0.244

2.258 ± 0.146

2.505 ± 0.11
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski