Burkholderia virus phi1026b

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Stanholtvirus

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6JII7|Q6JII7_9CAUD Gp44 OS=Burkholderia virus phi1026b OX=255131 PE=4 SV=1
MM1 pKa = 7.2TNEE4 pKa = 4.31TGSSATTDD12 pKa = 2.83VRR14 pKa = 11.84RR15 pKa = 11.84TVRR18 pKa = 11.84SKK20 pKa = 11.19DD21 pKa = 3.41NEE23 pKa = 4.2FFSCDD28 pKa = 3.62ALDD31 pKa = 4.36EE32 pKa = 4.51LLDD35 pKa = 3.68MHH37 pKa = 7.54DD38 pKa = 4.03EE39 pKa = 4.13LAVGDD44 pKa = 3.95VVFYY48 pKa = 11.28GDD50 pKa = 4.11VEE52 pKa = 4.7PIPIVRR58 pKa = 11.84LCDD61 pKa = 3.66ANDD64 pKa = 3.82VIDD67 pKa = 4.49MIGDD71 pKa = 3.8RR72 pKa = 11.84AYY74 pKa = 11.02DD75 pKa = 3.89EE76 pKa = 4.42VGEE79 pKa = 4.41AADD82 pKa = 5.22GYY84 pKa = 10.37PDD86 pKa = 3.56IAPEE90 pKa = 3.99AKK92 pKa = 10.54AEE94 pKa = 4.1LEE96 pKa = 4.22TLLSGWIEE104 pKa = 3.93KK105 pKa = 9.34HH106 pKa = 6.08AKK108 pKa = 6.75PTFYY112 pKa = 10.98SVVNVSEE119 pKa = 4.17YY120 pKa = 11.05VITAADD126 pKa = 3.57VEE128 pKa = 4.46GRR130 pKa = 11.84EE131 pKa = 4.25

Molecular weight: 14.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6JIF7|Q6JIF7_9CAUD Gp74 OS=Burkholderia virus phi1026b OX=255131 PE=4 SV=1
MM1 pKa = 7.63PVRR4 pKa = 11.84MWVEE8 pKa = 3.96IPDD11 pKa = 3.72GSYY14 pKa = 10.47SVPRR18 pKa = 11.84HH19 pKa = 5.98RR20 pKa = 11.84GRR22 pKa = 11.84GGIIVCEE29 pKa = 4.29RR30 pKa = 11.84KK31 pKa = 9.61RR32 pKa = 11.84EE33 pKa = 3.67IDD35 pKa = 3.15ATVFRR40 pKa = 11.84IARR43 pKa = 11.84IATVKK48 pKa = 10.11RR49 pKa = 11.84QLIAAVEE56 pKa = 4.01VDD58 pKa = 3.42AFIPEE63 pKa = 4.08MHH65 pKa = 7.45RR66 pKa = 11.84SRR68 pKa = 11.84IPEE71 pKa = 3.95CDD73 pKa = 3.55GRR75 pKa = 11.84WVGPGVFRR83 pKa = 11.84TKK85 pKa = 10.71AYY87 pKa = 8.2VHH89 pKa = 6.86RR90 pKa = 11.84NRR92 pKa = 11.84HH93 pKa = 5.46SGVLGAFIEE102 pKa = 4.76SGDD105 pKa = 4.0SAWDD109 pKa = 3.49VRR111 pKa = 11.84GMSS114 pKa = 3.4

Molecular weight: 12.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

17246

35

1354

207.8

22.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.252 ± 0.662

1.403 ± 0.181

5.926 ± 0.221

6.28 ± 0.288

3.189 ± 0.205

8.089 ± 0.303

1.856 ± 0.17

4.807 ± 0.196

4.175 ± 0.233

8.06 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.096

3.114 ± 0.141

4.581 ± 0.326

3.775 ± 0.217

7.631 ± 0.3

5.845 ± 0.211

5.265 ± 0.259

7.289 ± 0.315

1.693 ± 0.149

2.499 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski