Cnuibacter physcomitrellae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Cnuibacter

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4009 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9LP24|A0A1X9LP24_9MICO HTH deoR-type domain-containing protein OS=Cnuibacter physcomitrellae OX=1619308 GN=B5808_12960 PE=4 SV=1
MM1 pKa = 7.73SDD3 pKa = 3.86DD4 pKa = 4.16RR5 pKa = 11.84NNPGPQQSGEE15 pKa = 4.39DD16 pKa = 3.66GQQPSRR22 pKa = 11.84GADD25 pKa = 3.08RR26 pKa = 11.84TGDD29 pKa = 3.48LPAYY33 pKa = 8.11PVQGQGDD40 pKa = 4.27SGPAFPGYY48 pKa = 9.27PPQGQPGSGVPPQGPQSPNAAPPTGAPQPHH78 pKa = 7.43PGYY81 pKa = 10.03GQPQSPQPHH90 pKa = 7.27PGYY93 pKa = 10.53GPQPPTYY100 pKa = 9.5GQPQGQPPQSGPQPHH115 pKa = 7.39PGYY118 pKa = 10.17GQPQAQTPQWAQGAPNGSGRR138 pKa = 11.84PPKK141 pKa = 10.26KK142 pKa = 10.08GLSVGALIGIIGGGVFLLLVIGVIVAIVVIRR173 pKa = 11.84SVASPAGGGTSQSTSPSQVVTEE195 pKa = 4.22YY196 pKa = 10.02LTAIADD202 pKa = 3.75GDD204 pKa = 3.96AEE206 pKa = 4.28KK207 pKa = 11.04ALGYY211 pKa = 10.53LGTPPEE217 pKa = 4.63DD218 pKa = 3.54KK219 pKa = 10.79SLLSDD224 pKa = 3.63EE225 pKa = 4.57VLAASNEE232 pKa = 4.07LAPLTGVSVVTEE244 pKa = 4.12DD245 pKa = 3.54TSNGSSDD252 pKa = 3.51VTVTYY257 pKa = 10.67QLGAQPVTAEE267 pKa = 4.12YY268 pKa = 10.99SVLDD272 pKa = 3.94YY273 pKa = 11.45DD274 pKa = 6.1DD275 pKa = 5.75DD276 pKa = 4.71GVWEE280 pKa = 4.14ISGGTGYY287 pKa = 10.52ISTSKK292 pKa = 11.05FEE294 pKa = 4.35GLGLTINGTAVPDD307 pKa = 3.9GDD309 pKa = 3.84EE310 pKa = 4.44VEE312 pKa = 4.6VFPGSYY318 pKa = 10.2QLATTSANFTLSGDD332 pKa = 3.4ATVVVDD338 pKa = 4.76QPFGTADD345 pKa = 3.28TSDD348 pKa = 3.31ITPALTDD355 pKa = 3.2AALQQFRR362 pKa = 11.84GLVRR366 pKa = 11.84AAVEE370 pKa = 4.11GCIASTTLDD379 pKa = 3.96AGCGLAIPATLSDD392 pKa = 3.87GTQLTDD398 pKa = 3.26GTIQRR403 pKa = 11.84TLPADD408 pKa = 3.42TSTTIDD414 pKa = 3.6SLEE417 pKa = 4.03VTLSYY422 pKa = 11.34DD423 pKa = 3.93NPTLAQGEE431 pKa = 4.83SIGGIDD437 pKa = 4.56VSAQCTQGGQTGTCSVLFGPSLGRR461 pKa = 11.84PSVDD465 pKa = 2.8MASPNPTVLWDD476 pKa = 3.39

Molecular weight: 48.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9LIW4|A0A1X9LIW4_9MICO Aspartate aminotransferase family protein OS=Cnuibacter physcomitrellae OX=1619308 GN=B5808_07565 PE=3 SV=1
MM1 pKa = 6.76VRR3 pKa = 11.84VVRR6 pKa = 11.84GFRR9 pKa = 11.84LLGVVRR15 pKa = 11.84VVRR18 pKa = 11.84GFRR21 pKa = 11.84LLGVVRR27 pKa = 11.84VVRR30 pKa = 11.84GFGLLGVVRR39 pKa = 11.84VVRR42 pKa = 11.84GFGLLGVVRR51 pKa = 11.84VVRR54 pKa = 11.84GFGLLGVVRR63 pKa = 11.84VVRR66 pKa = 11.84GFGLLGVVMVVRR78 pKa = 11.84GFRR81 pKa = 11.84LLGVLMVVRR90 pKa = 11.84GGSARR95 pKa = 11.84SAWSWWSAGSACSAWW110 pKa = 3.34

Molecular weight: 11.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4009

0

4009

1285964

32

2550

320.8

34.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.394 ± 0.055

0.485 ± 0.007

6.328 ± 0.036

5.519 ± 0.039

3.008 ± 0.023

9.12 ± 0.037

1.872 ± 0.02

4.3 ± 0.031

1.696 ± 0.024

10.242 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.679 ± 0.013

1.756 ± 0.021

5.612 ± 0.03

2.695 ± 0.023

7.306 ± 0.047

6.195 ± 0.034

6.182 ± 0.043

9.171 ± 0.043

1.459 ± 0.016

1.981 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski