Soybean mild mottle virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D6MTW0|D6MTW0_9GEMI Capsid protein OS=Soybean mild mottle virus OX=761701 GN=V1 PE=3 SV=1
MM1 pKa = 8.07WDD3 pKa = 3.42PLQNPFPHH11 pKa = 6.22TVHH14 pKa = 7.28GLRR17 pKa = 11.84CMLAIKK23 pKa = 9.79YY24 pKa = 8.08VQLVIDD30 pKa = 4.98TYY32 pKa = 9.52PQDD35 pKa = 4.54SIGEE39 pKa = 4.07DD40 pKa = 3.65LLRR43 pKa = 11.84QIIQILRR50 pKa = 11.84CRR52 pKa = 11.84NHH54 pKa = 6.45DD55 pKa = 3.43QAEE58 pKa = 4.06FRR60 pKa = 11.84YY61 pKa = 10.08SILFADD67 pKa = 4.33VEE69 pKa = 4.54RR70 pKa = 11.84TEE72 pKa = 4.23KK73 pKa = 9.42TQLRR77 pKa = 11.84NPYY80 pKa = 8.5GTTCTCRR87 pKa = 11.84FCPKK91 pKa = 9.93HH92 pKa = 5.43VQAKK96 pKa = 9.45SLEE99 pKa = 4.49EE100 pKa = 3.83PTHH103 pKa = 4.74VQEE106 pKa = 4.71AQNLQDD112 pKa = 3.91VSFEE116 pKa = 4.14RR117 pKa = 11.84CSKK120 pKa = 10.81GVV122 pKa = 3.05

Molecular weight: 14.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D6MTW4|D6MTW4_9GEMI C4 protein OS=Soybean mild mottle virus OX=761701 GN=C4 PE=3 SV=1
MM1 pKa = 7.57GNHH4 pKa = 6.58ISMCLSSLKK13 pKa = 10.53EE14 pKa = 4.16SVKK17 pKa = 10.63LRR19 pKa = 11.84TKK21 pKa = 9.86DD22 pKa = 3.09TSTSLPQEE30 pKa = 4.29GQHH33 pKa = 6.1ISIRR37 pKa = 11.84TFRR40 pKa = 11.84EE41 pKa = 3.7LNQVRR46 pKa = 11.84TSKK49 pKa = 8.94HH50 pKa = 4.84TSTKK54 pKa = 10.45TEE56 pKa = 3.77TPYY59 pKa = 11.24NGGLSRR65 pKa = 11.84LMVEE69 pKa = 4.46VPEE72 pKa = 4.73EE73 pKa = 4.02VAKK76 pKa = 10.45MLTTHH81 pKa = 5.59VRR83 pKa = 11.84RR84 pKa = 11.84HH85 pKa = 4.71

Molecular weight: 9.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1092

85

349

182.0

21.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.67 ± 1.085

2.839 ± 0.386

4.67 ± 0.442

5.403 ± 0.882

4.945 ± 0.765

5.22 ± 0.52

3.663 ± 0.487

5.128 ± 0.988

6.136 ± 0.691

7.326 ± 1.344

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.022 ± 0.651

5.128 ± 0.621

4.945 ± 0.64

4.762 ± 0.682

7.143 ± 1.067

8.15 ± 0.773

5.678 ± 0.918

5.952 ± 0.733

1.282 ± 0.201

3.938 ± 0.82
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski