Pectinobacterium phage PEAT2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peatvirus; Pectinobacterium virus PEAT2

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4N7B0|A0A2H4N7B0_9CAUD Tail fiber protein OS=Pectinobacterium phage PEAT2 OX=2053078 PE=4 SV=1
MM1 pKa = 7.92AEE3 pKa = 4.19LTSTGYY9 pKa = 9.8SVKK12 pKa = 10.61SQNDD16 pKa = 3.18WFDD19 pKa = 3.78EE20 pKa = 4.22EE21 pKa = 4.11QQLYY25 pKa = 10.95RR26 pKa = 11.84NIDD29 pKa = 3.89SNWNLDD35 pKa = 3.15PSTPDD40 pKa = 3.47GLKK43 pKa = 9.09MAHH46 pKa = 6.79DD47 pKa = 4.33AEE49 pKa = 4.65IFSALDD55 pKa = 3.24EE56 pKa = 4.48VLQQAYY62 pKa = 10.17NSKK65 pKa = 10.83DD66 pKa = 3.36PNKK69 pKa = 10.71ASGYY73 pKa = 10.2DD74 pKa = 3.51LDD76 pKa = 4.94VICALTGTVRR86 pKa = 11.84SEE88 pKa = 4.03GTASTVTGFVLTGTPGTQVPAGTRR112 pKa = 11.84FEE114 pKa = 4.52SSVTGYY120 pKa = 10.68RR121 pKa = 11.84FALDD125 pKa = 3.44QTWTLDD131 pKa = 3.37SSGTATVDD139 pKa = 3.23ITCTTVGEE147 pKa = 4.34IEE149 pKa = 4.58ADD151 pKa = 3.23ANTITTIVDD160 pKa = 3.73TVAGLVSVNNPTPATPGTAAEE181 pKa = 4.37SDD183 pKa = 3.2GSLRR187 pKa = 11.84LKK189 pKa = 10.49RR190 pKa = 11.84ATAVGLPGSNQVDD203 pKa = 3.73SMLGQLFNVDD213 pKa = 2.55GVRR216 pKa = 11.84RR217 pKa = 11.84VRR219 pKa = 11.84VYY221 pKa = 11.13EE222 pKa = 4.0NDD224 pKa = 3.23EE225 pKa = 4.11AATDD229 pKa = 3.92SNGQPGHH236 pKa = 6.57SIAPIIDD243 pKa = 3.7GGTDD247 pKa = 3.56DD248 pKa = 4.92DD249 pKa = 4.12VAMAIYY255 pKa = 10.12LKK257 pKa = 10.46KK258 pKa = 10.73NPGVTLYY265 pKa = 10.68QAGTDD270 pKa = 3.41VTVTVTSPTYY280 pKa = 8.95PTMTKK285 pKa = 9.97DD286 pKa = 3.14IKK288 pKa = 10.47FSRR291 pKa = 11.84PVYY294 pKa = 10.01VDD296 pKa = 2.79MVVAIEE302 pKa = 4.23IKK304 pKa = 10.56DD305 pKa = 3.92DD306 pKa = 3.79GSLPSQVTLEE316 pKa = 4.45PLIQDD321 pKa = 4.96AIMEE325 pKa = 4.3YY326 pKa = 10.66AAGGLIPTEE335 pKa = 4.23YY336 pKa = 10.73GFKK339 pKa = 10.12PDD341 pKa = 3.92GFDD344 pKa = 2.96IGEE347 pKa = 4.3TVPYY351 pKa = 10.09SSLYY355 pKa = 9.26TPINKK360 pKa = 9.51VIGSYY365 pKa = 10.9GNSYY369 pKa = 9.48VNSMTLNSGTANVTIDD385 pKa = 3.64FNEE388 pKa = 4.07LSRR391 pKa = 11.84WTASNITVTIVV402 pKa = 2.6

Molecular weight: 42.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4N7D0|A0A2H4N7D0_9CAUD Uncharacterized protein OS=Pectinobacterium phage PEAT2 OX=2053078 PE=4 SV=1
MM1 pKa = 7.56SFGLRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84HH10 pKa = 4.89VPSAFSALLVEE21 pKa = 5.54WIRR24 pKa = 11.84SSHH27 pKa = 5.05GVPVEE32 pKa = 4.25STGSAISSTVAMYY45 pKa = 9.55PCEE48 pKa = 4.23KK49 pKa = 10.32LPSPTGSGSPTNFCSVIMCVAFGTTSASTCCAVVSISGSLRR90 pKa = 11.84LPCNSSPSKK99 pKa = 10.28RR100 pKa = 11.84ASCIMRR106 pKa = 11.84GAAFINSPVAVFKK119 pKa = 10.89RR120 pKa = 11.84SNAAVASVVSMFNLYY135 pKa = 10.37DD136 pKa = 3.48SSPAVVLRR144 pKa = 11.84GSAPCASAISSSALPNARR162 pKa = 11.84SAMWSSFGMVKK173 pKa = 10.21VIIIWTPVVRR183 pKa = 11.84ILHH186 pKa = 5.68ACKK189 pKa = 9.99KK190 pKa = 10.1LRR192 pKa = 11.84YY193 pKa = 8.98LRR195 pKa = 11.84CC196 pKa = 3.75

Molecular weight: 20.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

14633

107

820

266.1

29.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.939 ± 0.38

1.633 ± 0.199

6.109 ± 0.249

5.078 ± 0.281

3.724 ± 0.202

7.456 ± 0.37

1.989 ± 0.217

5.74 ± 0.191

4.77 ± 0.332

7.469 ± 0.247

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.857 ± 0.17

4.852 ± 0.279

4.353 ± 0.228

3.82 ± 0.258

5.597 ± 0.335

6.465 ± 0.309

6.745 ± 0.304

7.497 ± 0.262

1.667 ± 0.141

3.239 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski