Neodiprion lecontei nucleopolyhedrovirus (strain Canada) (NeleNPV)

Taxonomy: Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Gammabaculovirus; Neodiprion lecontei nucleopolyhedrovirus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6JP86|Q6JP86_NPVNC Uncharacterized protein OS=Neodiprion lecontei nucleopolyhedrovirus (strain Canada) OX=654906 PE=4 SV=1
MM1 pKa = 7.18FLIVFLFLCILLLFFTYY18 pKa = 10.95NFFVDD23 pKa = 3.24NTDD26 pKa = 3.03NVEE29 pKa = 4.11KK30 pKa = 10.74YY31 pKa = 10.2KK32 pKa = 10.65QIHH35 pKa = 5.14XLLMEE40 pKa = 5.84AYY42 pKa = 8.67TSDD45 pKa = 3.74IISFDD50 pKa = 3.28NVSFISRR57 pKa = 11.84VRR59 pKa = 11.84WPVYY63 pKa = 9.66EE64 pKa = 3.99VVTFEE69 pKa = 4.31TEE71 pKa = 4.31TYY73 pKa = 10.89TMTSNMWYY81 pKa = 10.15SGLEE85 pKa = 3.76KK86 pKa = 10.49RR87 pKa = 11.84FNFYY91 pKa = 11.01DD92 pKa = 3.38QTFEE96 pKa = 5.16DD97 pKa = 3.82VDD99 pKa = 4.44DD100 pKa = 3.83NTEE103 pKa = 3.88RR104 pKa = 11.84VTTIDD109 pKa = 3.09ATSFSLHH116 pKa = 6.7VDD118 pKa = 3.8DD119 pKa = 5.72GWVDD123 pKa = 4.3LKK125 pKa = 11.34CPANMIFDD133 pKa = 5.21GYY135 pKa = 10.73QCVRR139 pKa = 11.84QNICEE144 pKa = 4.04NQSAGTILPVNEE156 pKa = 4.31SLIDD160 pKa = 3.11IAFYY164 pKa = 10.69QKK166 pKa = 10.9YY167 pKa = 9.73NRR169 pKa = 11.84STTTDD174 pKa = 3.78TIYY177 pKa = 10.88HH178 pKa = 5.49PTLYY182 pKa = 9.83IVCNGDD188 pKa = 3.13DD189 pKa = 3.44SYY191 pKa = 11.72EE192 pKa = 4.15IKK194 pKa = 10.77EE195 pKa = 4.38CDD197 pKa = 3.6DD198 pKa = 3.63NQLFDD203 pKa = 5.89SDD205 pKa = 4.13MLQCVDD211 pKa = 4.76KK212 pKa = 10.96DD213 pKa = 3.45ACADD217 pKa = 3.49VADD220 pKa = 4.28YY221 pKa = 10.35FVLSDD226 pKa = 4.96RR227 pKa = 11.84PDD229 pKa = 3.29TLADD233 pKa = 4.4DD234 pKa = 4.76EE235 pKa = 5.75YY236 pKa = 11.89LMCLNNEE243 pKa = 4.37TTVQTCDD250 pKa = 2.75EE251 pKa = 4.16NMIFDD256 pKa = 4.36TTTLGCVEE264 pKa = 4.36VDD266 pKa = 3.11LCKK269 pKa = 10.5INGSGYY275 pKa = 9.34TYY277 pKa = 9.61ITDD280 pKa = 4.14DD281 pKa = 4.26LADD284 pKa = 3.56NQYY287 pKa = 9.73YY288 pKa = 9.68QCTSAFDD295 pKa = 3.77KK296 pKa = 11.14QIISCPIRR304 pKa = 11.84VEE306 pKa = 3.72TDD308 pKa = 3.01GQYY311 pKa = 11.11SCAGDD316 pKa = 3.85TRR318 pKa = 11.84CMSLEE323 pKa = 4.17NGSGSIMTMIEE334 pKa = 3.95NTHH337 pKa = 5.44FRR339 pKa = 11.84YY340 pKa = 10.48ANEE343 pKa = 4.01AVVCTDD349 pKa = 3.24YY350 pKa = 11.33EE351 pKa = 4.14ITDD354 pKa = 3.79SVVCDD359 pKa = 3.61SEE361 pKa = 5.23EE362 pKa = 3.94IEE364 pKa = 4.31LTVEE368 pKa = 4.47PFTLDD373 pKa = 3.19LXIPQIILDD382 pKa = 4.0RR383 pKa = 11.84TTNICKK389 pKa = 10.41SFDD392 pKa = 3.54INDD395 pKa = 3.33VEE397 pKa = 5.44IITDD401 pKa = 3.39HH402 pKa = 6.63AGIVSTEE409 pKa = 3.23NDD411 pKa = 3.55YY412 pKa = 11.7NVDD415 pKa = 3.41FTTAVRR421 pKa = 11.84VNVQNLLSKK430 pKa = 10.15NLPVSTSMLFSSLDD444 pKa = 3.32YY445 pKa = 10.88AKK447 pKa = 10.67NYY449 pKa = 10.01GFIGFSPLTGNEE461 pKa = 3.6IDD463 pKa = 4.18CYY465 pKa = 11.39GXVLYY470 pKa = 10.58DD471 pKa = 3.43IFAGDD476 pKa = 4.17KK477 pKa = 11.25YY478 pKa = 11.08NICSDD483 pKa = 3.96NEE485 pKa = 4.13ITSSNVIXDD494 pKa = 4.02NEE496 pKa = 4.12FFSTYY501 pKa = 9.26TNTVGTTSITSPCRR515 pKa = 11.84VKK517 pKa = 11.03SDD519 pKa = 2.99IVLDD523 pKa = 3.5NDD525 pKa = 3.97IYY527 pKa = 10.83IQEE530 pKa = 4.15SDD532 pKa = 3.74NVSVSCLYY540 pKa = 11.18AMPLQEE546 pKa = 6.1IIVTDD551 pKa = 4.09LAEE554 pKa = 4.25TAGVLVNDD562 pKa = 3.74EE563 pKa = 4.33CFEE566 pKa = 4.72NGIDD570 pKa = 3.86LNNVAGAIVILNDD583 pKa = 3.2EE584 pKa = 4.31LACRR588 pKa = 11.84SVYY591 pKa = 10.36VEE593 pKa = 4.53SYY595 pKa = 9.05NTIITASFTPVMKK608 pKa = 10.69FEE610 pKa = 3.99EE611 pKa = 4.54RR612 pKa = 11.84TWNVDD617 pKa = 3.12TQSYY621 pKa = 10.09DD622 pKa = 2.77GVMYY626 pKa = 10.6DD627 pKa = 3.81SVLHH631 pKa = 5.73KK632 pKa = 7.79TTNGWLACPPSLYY645 pKa = 10.73DD646 pKa = 3.97EE647 pKa = 4.42TTMDD651 pKa = 4.56CNIDD655 pKa = 3.33KK656 pKa = 10.24TKK658 pKa = 10.85LYY660 pKa = 9.73TIRR663 pKa = 11.84DD664 pKa = 3.44LHH666 pKa = 6.28FVQNYY671 pKa = 9.38PDD673 pKa = 3.74IDD675 pKa = 3.94LEE677 pKa = 4.4AEE679 pKa = 3.9NEE681 pKa = 4.11INIDD685 pKa = 3.45TDD687 pKa = 4.19DD688 pKa = 4.11VDD690 pKa = 3.75VSNPIVDD697 pKa = 4.58EE698 pKa = 4.24IEE700 pKa = 4.68AINPIVDD707 pKa = 3.61DD708 pKa = 3.93VVLRR712 pKa = 11.84RR713 pKa = 11.84INNATNNIHH722 pKa = 7.44PILEE726 pKa = 4.26NKK728 pKa = 9.07VVSDD732 pKa = 3.93STQMTLDD739 pKa = 3.47KK740 pKa = 10.57KK741 pKa = 10.7EE742 pKa = 4.1PSIDD746 pKa = 3.61VQDD749 pKa = 3.79IDD751 pKa = 4.06STSTSSNEE759 pKa = 3.83SAEE762 pKa = 4.21NEE764 pKa = 4.43STVLDD769 pKa = 3.92PSKK772 pKa = 10.59VLEE775 pKa = 4.4YY776 pKa = 10.34INRR779 pKa = 11.84YY780 pKa = 8.98HH781 pKa = 7.41EE782 pKa = 4.69SDD784 pKa = 3.08TSEE787 pKa = 4.08EE788 pKa = 4.04EE789 pKa = 4.4EE790 pKa = 4.52YY791 pKa = 10.55MDD793 pKa = 6.01DD794 pKa = 4.55SDD796 pKa = 4.37ILASLFEE803 pKa = 4.25

Molecular weight: 90.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6JPE2|Q6JPE2_NPVNC Uncharacterized protein OS=Neodiprion lecontei nucleopolyhedrovirus (strain Canada) OX=654906 PE=4 SV=1
MM1 pKa = 7.92VSEE4 pKa = 4.59SSDD7 pKa = 3.28SKK9 pKa = 11.53KK10 pKa = 10.0EE11 pKa = 3.91LKK13 pKa = 10.16PIEE16 pKa = 3.71IMAYY20 pKa = 8.29NRR22 pKa = 11.84RR23 pKa = 11.84PGRR26 pKa = 11.84PRR28 pKa = 11.84KK29 pKa = 9.48RR30 pKa = 11.84RR31 pKa = 11.84SYY33 pKa = 11.43GNNSTNRR40 pKa = 11.84TRR42 pKa = 11.84SRR44 pKa = 11.84SRR46 pKa = 11.84SRR48 pKa = 11.84SRR50 pKa = 11.84SRR52 pKa = 11.84SRR54 pKa = 11.84SRR56 pKa = 11.84SRR58 pKa = 11.84TGGRR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84SRR66 pKa = 11.84SSSSYY71 pKa = 8.19NRR73 pKa = 11.84NYY75 pKa = 10.37RR76 pKa = 11.84SSNSPSSNRR85 pKa = 11.84QYY87 pKa = 11.02GRR89 pKa = 11.84SRR91 pKa = 11.84SRR93 pKa = 11.84SRR95 pKa = 11.84SLSQRR100 pKa = 11.84RR101 pKa = 11.84YY102 pKa = 9.37

Molecular weight: 12.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

24124

52

1134

271.1

31.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.465 ± 0.206

2.636 ± 0.163

6.587 ± 0.208

5.186 ± 0.2

5.007 ± 0.204

2.922 ± 0.198

2.413 ± 0.152

8.983 ± 0.187

7.184 ± 0.299

8.966 ± 0.194

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.317 ± 0.093

8.079 ± 0.233

2.657 ± 0.133

3.457 ± 0.128

4.149 ± 0.217

6.997 ± 0.22

6.848 ± 0.23

6.334 ± 0.164

0.767 ± 0.071

4.825 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski