Lloviu cuevavirus (isolate Bat/Spain/Asturias-Bat86/2003)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Filoviridae; Cuevavirus; Lloviu cuevavirus

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8EFI2|G8EFI2_LLOVA Polymerase cofactor VP35 OS=Lloviu cuevavirus (isolate Bat/Spain/Asturias-Bat86/2003) OX=1559366 PE=3 SV=1
MM1 pKa = 7.21NRR3 pKa = 11.84YY4 pKa = 8.91LGHH7 pKa = 5.53GTRR10 pKa = 11.84TSRR13 pKa = 11.84EE14 pKa = 3.84NTNLSEE20 pKa = 3.96LHH22 pKa = 6.72GILSLGLNVDD32 pKa = 3.34HH33 pKa = 7.02TIVRR37 pKa = 11.84KK38 pKa = 10.07KK39 pKa = 10.54SIPLFEE45 pKa = 6.29IGNSDD50 pKa = 4.38QVCNWIIQIIEE61 pKa = 4.34AGVDD65 pKa = 3.71LQDD68 pKa = 3.68VADD71 pKa = 4.29SFLTMLCVNHH81 pKa = 7.52AYY83 pKa = 10.37QGDD86 pKa = 3.94PNLFLEE92 pKa = 5.02SPAAHH97 pKa = 6.21YY98 pKa = 10.69LKK100 pKa = 10.76GHH102 pKa = 7.16GIHH105 pKa = 6.76FEE107 pKa = 3.74IQHH110 pKa = 6.64RR111 pKa = 11.84DD112 pKa = 3.64NVDD115 pKa = 3.73HH116 pKa = 6.74ITDD119 pKa = 4.08LLGVGSRR126 pKa = 11.84DD127 pKa = 2.95KK128 pKa = 11.13SLRR131 pKa = 11.84KK132 pKa = 7.86TLSALEE138 pKa = 4.21FEE140 pKa = 5.27PDD142 pKa = 3.2GSTTAGMFLSFASLFLPKK160 pKa = 10.36LVVGEE165 pKa = 4.29RR166 pKa = 11.84ACLEE170 pKa = 3.85KK171 pKa = 10.79VQRR174 pKa = 11.84QIQIHH179 pKa = 6.28AEE181 pKa = 3.79QGLIQYY187 pKa = 7.39PTQWQSVGHH196 pKa = 5.02MMVVFRR202 pKa = 11.84LIRR205 pKa = 11.84VNFVLKK211 pKa = 10.4FLLVHH216 pKa = 6.24QGMHH220 pKa = 5.31MMAGHH225 pKa = 7.48DD226 pKa = 3.95ANDD229 pKa = 4.4AIIANSISQTRR240 pKa = 11.84FSGLLIVKK248 pKa = 8.18TVLEE252 pKa = 5.03HH253 pKa = 7.17ILQKK257 pKa = 10.01TEE259 pKa = 4.38AGVQLHH265 pKa = 6.13PLARR269 pKa = 11.84TSKK272 pKa = 10.79VKK274 pKa = 11.11GEE276 pKa = 3.92LLAFKK281 pKa = 10.58SALEE285 pKa = 3.83ALASHH290 pKa = 7.08RR291 pKa = 11.84EE292 pKa = 3.95YY293 pKa = 11.47APFARR298 pKa = 11.84LLNLSGVNNLEE309 pKa = 4.28HH310 pKa = 6.95GLYY313 pKa = 9.39PQLSAIALGVATAHH327 pKa = 6.58GSTLAGVNVSEE338 pKa = 5.05QYY340 pKa = 9.67QQLRR344 pKa = 11.84EE345 pKa = 4.06AATEE349 pKa = 4.1AEE351 pKa = 4.48KK352 pKa = 10.71QLQQHH357 pKa = 5.3SEE359 pKa = 3.85MRR361 pKa = 11.84EE362 pKa = 4.03LEE364 pKa = 4.17TLGLDD369 pKa = 3.34EE370 pKa = 4.89QEE372 pKa = 4.29RR373 pKa = 11.84KK374 pKa = 9.7ILATFHH380 pKa = 6.2SRR382 pKa = 11.84KK383 pKa = 9.96NEE385 pKa = 3.52INIQQTSSILAIRR398 pKa = 11.84KK399 pKa = 7.82EE400 pKa = 3.98RR401 pKa = 11.84LRR403 pKa = 11.84KK404 pKa = 8.52LTEE407 pKa = 3.95ALNEE411 pKa = 4.21EE412 pKa = 4.49KK413 pKa = 10.82NKK415 pKa = 10.42NALDD419 pKa = 4.45DD420 pKa = 4.21EE421 pKa = 5.08DD422 pKa = 5.18EE423 pKa = 4.68SEE425 pKa = 4.24EE426 pKa = 5.15DD427 pKa = 3.42DD428 pKa = 3.44WSPEE432 pKa = 3.61NRR434 pKa = 11.84GIRR437 pKa = 11.84SNKK440 pKa = 9.56GSTKK444 pKa = 10.16EE445 pKa = 4.03SSSYY449 pKa = 8.5TASRR453 pKa = 11.84TEE455 pKa = 3.8EE456 pKa = 3.72DD457 pKa = 3.46RR458 pKa = 11.84NNYY461 pKa = 8.45NSKK464 pKa = 10.57DD465 pKa = 3.42DD466 pKa = 3.96HH467 pKa = 7.59LSGKK471 pKa = 8.05EE472 pKa = 3.87QMSTQQEE479 pKa = 4.33SGADD483 pKa = 3.7DD484 pKa = 5.59LDD486 pKa = 5.68LFDD489 pKa = 6.82LDD491 pKa = 6.14DD492 pKa = 6.19DD493 pKa = 5.49GDD495 pKa = 4.32TNSQDD500 pKa = 3.2PNNRR504 pKa = 11.84QKK506 pKa = 10.81QSDD509 pKa = 3.86TQQTQEE515 pKa = 3.72SSDD518 pKa = 3.36RR519 pKa = 11.84SDD521 pKa = 3.4YY522 pKa = 11.07SRR524 pKa = 11.84RR525 pKa = 11.84PAYY528 pKa = 9.7DD529 pKa = 3.28WPPGDD534 pKa = 4.84RR535 pKa = 11.84PHH537 pKa = 6.25TTQATDD543 pKa = 2.78EE544 pKa = 4.44HH545 pKa = 6.69TDD547 pKa = 3.96LLNKK551 pKa = 9.32DD552 pKa = 3.24HH553 pKa = 7.36RR554 pKa = 11.84RR555 pKa = 11.84NQVKK559 pKa = 9.86PGRR562 pKa = 11.84RR563 pKa = 11.84GNDD566 pKa = 3.0PRR568 pKa = 11.84TLPLISFDD576 pKa = 4.27DD577 pKa = 4.1NEE579 pKa = 5.12GEE581 pKa = 4.22ILDD584 pKa = 4.99DD585 pKa = 5.54KK586 pKa = 11.54SDD588 pKa = 3.8LPAPDD593 pKa = 3.54THH595 pKa = 7.21SDD597 pKa = 3.43PDD599 pKa = 3.84TEE601 pKa = 4.33EE602 pKa = 4.64SEE604 pKa = 4.71EE605 pKa = 3.99EE606 pKa = 4.28HH607 pKa = 6.93PDD609 pKa = 3.86EE610 pKa = 4.85EE611 pKa = 4.91LLPPAPKK618 pKa = 10.77YY619 pKa = 7.79NTKK622 pKa = 9.98TSEE625 pKa = 3.99QEE627 pKa = 3.73PGDD630 pKa = 3.69WKK632 pKa = 11.1QPTSPLSTIPEE643 pKa = 4.09EE644 pKa = 4.46EE645 pKa = 4.69GGHH648 pKa = 5.56EE649 pKa = 4.06ANNDD653 pKa = 3.25NSEE656 pKa = 4.1SDD658 pKa = 5.62LIDD661 pKa = 3.55QMYY664 pKa = 9.59QHH666 pKa = 6.88IFEE669 pKa = 4.7TEE671 pKa = 3.46GAYY674 pKa = 10.18AAINYY679 pKa = 7.99YY680 pKa = 10.61YY681 pKa = 9.06KK682 pKa = 8.5TTGRR686 pKa = 11.84PVTFTSNNNHH696 pKa = 7.38DD697 pKa = 3.72YY698 pKa = 10.63TFPQDD703 pKa = 3.33IEE705 pKa = 4.75GLFPPWEE712 pKa = 4.21GKK714 pKa = 9.85EE715 pKa = 3.98NQKK718 pKa = 10.1VAEE721 pKa = 4.13ILTNSLHH728 pKa = 5.62EE729 pKa = 4.7TGQEE733 pKa = 3.82WADD736 pKa = 3.43MSAKK740 pKa = 9.54EE741 pKa = 4.54RR742 pKa = 11.84YY743 pKa = 9.52LFLINNN749 pKa = 4.44

Molecular weight: 84.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8EFI9|G8EFI9_LLOVA RNA-directed RNA polymerase L OS=Lloviu cuevavirus (isolate Bat/Spain/Asturias-Bat86/2003) OX=1559366 PE=3 SV=1
MM1 pKa = 7.51ARR3 pKa = 11.84PSGRR7 pKa = 11.84YY8 pKa = 9.24NILQTDD14 pKa = 4.17TFHH17 pKa = 6.89EE18 pKa = 4.38NTVVKK23 pKa = 10.56HH24 pKa = 5.83DD25 pKa = 3.91LCNFLVTTTITGWDD39 pKa = 4.09VYY41 pKa = 9.68WAGHH45 pKa = 6.71LFHH48 pKa = 6.95VPNKK52 pKa = 10.29GIALLTRR59 pKa = 11.84LKK61 pKa = 10.22TSDD64 pKa = 4.29FAPAWTLTKK73 pKa = 10.82NLFPHH78 pKa = 7.2LFHH81 pKa = 7.27NPTSTLKK88 pKa = 11.08DD89 pKa = 3.74PVWGLRR95 pKa = 11.84VILAAALYY103 pKa = 9.02DD104 pKa = 3.62QVMEE108 pKa = 4.07QSLIKK113 pKa = 10.0PLSEE117 pKa = 3.86TLTLLGDD124 pKa = 3.64WLLTTEE130 pKa = 4.22TQKK133 pKa = 10.93FNLRR137 pKa = 11.84TPRR140 pKa = 11.84AQEE143 pKa = 3.9QISLRR148 pKa = 11.84MLSLVKK154 pKa = 10.45KK155 pKa = 10.01HH156 pKa = 5.81VDD158 pKa = 3.19NFILKK163 pKa = 10.18LLEE166 pKa = 3.69LHH168 pKa = 6.84VINNRR173 pKa = 11.84GFTSSIEE180 pKa = 3.94IGNKK184 pKa = 9.43CNTIIISRR192 pKa = 11.84TNMGYY197 pKa = 10.27LVEE200 pKa = 4.08QQEE203 pKa = 4.31PDD205 pKa = 3.03KK206 pKa = 11.42SALRR210 pKa = 11.84KK211 pKa = 7.52TKK213 pKa = 9.94PGPVKK218 pKa = 9.85FTLVHH223 pKa = 6.47EE224 pKa = 4.45DD225 pKa = 2.91AFRR228 pKa = 11.84DD229 pKa = 4.68FKK231 pKa = 10.99PEE233 pKa = 3.64RR234 pKa = 11.84SSINLLIMEE243 pKa = 5.2FNSSLAII250 pKa = 4.01

Molecular weight: 28.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

5096

189

2196

566.2

63.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.221 ± 0.353

1.982 ± 0.429

4.749 ± 0.502

5.475 ± 0.646

3.65 ± 0.548

5.926 ± 0.832

3.159 ± 0.293

5.73 ± 0.547

4.847 ± 0.471

11.676 ± 0.783

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.805 ± 0.096

4.553 ± 0.469

5.946 ± 0.713

4.611 ± 0.473

5.514 ± 0.35

7.673 ± 0.464

6.888 ± 0.804

4.827 ± 0.442

1.648 ± 0.349

3.12 ± 0.559
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski